Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEN9T_RS11680 Genome accession   NZ_CP168610
Coordinates   640595..641734 (+) Length   379 a.a.
NCBI ID   WP_092972473.1    Uniprot ID   A0AAE3I1G5
Organism   Ralstonia sp. Ralssp110 strain CT11-110     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 635595..646734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN9T_RS11660 (ACEN9T_11665) ubiA 636860..637735 (+) 876 WP_413707779.1 4-hydroxybenzoate octaprenyltransferase -
  ACEN9T_RS11665 (ACEN9T_11670) proC 637790..638614 (-) 825 WP_413707780.1 pyrroline-5-carboxylate reductase -
  ACEN9T_RS11670 (ACEN9T_11675) - 638645..639361 (-) 717 WP_413707781.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEN9T_RS11675 (ACEN9T_11680) pilT 639484..640527 (+) 1044 WP_009241469.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEN9T_RS11680 (ACEN9T_11685) pilU 640595..641734 (+) 1140 WP_092972473.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEN9T_RS11685 (ACEN9T_11690) - 641932..642369 (+) 438 WP_413707782.1 type IV pilin protein -
  ACEN9T_RS11690 (ACEN9T_11695) - 642378..642851 (+) 474 WP_045202622.1 GspH/FimT family pseudopilin -
  ACEN9T_RS11695 (ACEN9T_11700) pilV 642848..643450 (+) 603 WP_413707783.1 type IV pilus modification protein PilV -
  ACEN9T_RS11700 (ACEN9T_11705) - 643447..644484 (+) 1038 WP_092972476.1 PilW family protein -
  ACEN9T_RS11705 (ACEN9T_11710) - 644487..645056 (+) 570 WP_092972477.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42299.60 Da        Isoelectric Point: 6.6639

>NTDB_id=1044607 ACEN9T_RS11680 WP_092972473.1 640595..641734(+) (pilU) [Ralstonia sp. Ralssp110 strain CT11-110]
MLDREAATKYIHELLQLMVNSRGSDLFITSEFPPAIKVDGKVTPISQQPLNAVQAVGLVKSIMNEKQLREYEDSSECNFA
ITAPGAGRFRVSAFMQQGRAGMVLRTINTKIPTLGELDLPPILNEIVMSKRGLVIVVGATGSGKSTSLAAMVGYRNANSY
GHIITIEDPVEYVHTHQNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
SNQAIDRIINFFPEEKRQQLLIDLSLNLRAMIAQRLLPRQGRKGRVPAVEIMLATPLVQDLIFKGEVHELKEVMKKSREQ
GMISFDQALFELYEENKITYEDALRNADSLNDLRLQIKLHSKRGGQTDLSAGTEHLNVV

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1044607 ACEN9T_RS11680 WP_092972473.1 640595..641734(+) (pilU) [Ralstonia sp. Ralssp110 strain CT11-110]
ATGCTTGACCGCGAAGCCGCCACCAAATACATCCACGAACTCTTGCAGTTGATGGTGAACAGCCGTGGCTCGGACTTGTT
CATCACGTCGGAATTTCCGCCCGCGATCAAGGTGGACGGCAAGGTCACGCCGATTTCGCAGCAGCCGCTGAATGCCGTGC
AGGCCGTCGGTCTGGTCAAGTCGATCATGAACGAGAAGCAGCTGCGCGAGTACGAAGACAGCTCGGAATGCAACTTCGCC
ATCACGGCGCCGGGTGCGGGGCGTTTCCGTGTGTCGGCGTTCATGCAGCAGGGCCGCGCCGGCATGGTGCTGCGGACCAT
CAACACGAAGATCCCGACGCTGGGCGAGCTGGATTTGCCGCCGATCCTGAACGAGATCGTGATGAGCAAGCGCGGGCTGG
TGATCGTGGTGGGTGCCACGGGCTCGGGCAAGTCGACCTCGCTGGCGGCGATGGTGGGCTATCGCAACGCGAATTCGTAT
GGCCACATCATCACCATCGAAGATCCGGTGGAATACGTGCACACCCACCAGAACTGCGTGGTGACGCAGCGTGAAGTGGG
CGTGGATACCGAGTCGTGGCACGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGATGTGATCCTGATCGGCGAAATCC
GGGATCGCGACACGATGGAATACGCCATCCAGTACGCGGAAACCGGCCACTTGTGCCTCGCCACGCTGCACGCCAACAGC
TCAAACCAAGCGATCGACCGGATCATCAACTTCTTCCCGGAAGAGAAGCGTCAGCAGTTGCTGATCGACCTGTCGCTGAA
CCTGCGCGCGATGATCGCGCAGCGCCTGCTGCCACGTCAGGGCAGAAAAGGGCGCGTGCCGGCCGTCGAAATCATGCTGG
CCACCCCGCTGGTGCAAGACCTCATCTTCAAGGGCGAGGTGCACGAGCTGAAGGAGGTCATGAAGAAATCCCGCGAGCAA
GGGATGATCTCGTTCGATCAGGCGTTGTTCGAGCTGTACGAGGAAAACAAGATCACTTACGAGGACGCGTTGCGCAATGC
GGACTCCCTCAACGATCTGCGCTTGCAGATCAAGCTGCACAGCAAGCGTGGCGGGCAGACGGATCTGTCTGCTGGCACCG
AGCATCTCAACGTCGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.05

95.515

0.583
  pilU Acinetobacter baylyi ADP1

55.143

92.348

0.509
  pilU Vibrio cholerae strain A1552

53.107

93.404

0.496
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.323

88.918

0.385
  pilT Acinetobacter baylyi ADP1

43.544

87.863

0.383
  pilT Vibrio cholerae strain A1552

45.426

83.641

0.38
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.426

83.641

0.38
  pilT Acinetobacter baumannii D1279779

43.243

87.863

0.38
  pilT Acinetobacter nosocomialis M2

43.243

87.863

0.38
  pilT Acinetobacter baumannii strain A118

43.243

87.863

0.38
  pilT Legionella pneumophila strain ERS1305867

42.943

87.863

0.377
  pilT Legionella pneumophila strain Lp02

42.943

87.863

0.377
  pilT Pseudomonas stutzeri DSM 10701

42.136

88.918

0.375
  pilT Pseudomonas aeruginosa PAK

42.136

88.918

0.375

Multiple sequence alignment