Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACEN9H_RS25430 Genome accession   NZ_CP168562
Coordinates   5807603..5808646 (+) Length   347 a.a.
NCBI ID   WP_160409673.1    Uniprot ID   -
Organism   Massilia cellulosiltytica strain CT11-92     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5802603..5813646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN9H_RS25405 (ACEN9H_25410) - 5803960..5804961 (+) 1002 WP_413672520.1 family 43 glycosylhydrolase -
  ACEN9H_RS25410 (ACEN9H_25415) - 5805031..5805408 (+) 378 WP_413672521.1 hypothetical protein -
  ACEN9H_RS25415 (ACEN9H_25420) - 5805405..5805896 (-) 492 WP_413672522.1 hypothetical protein -
  ACEN9H_RS25420 (ACEN9H_25425) proC 5805898..5806716 (-) 819 WP_160409671.1 pyrroline-5-carboxylate reductase -
  ACEN9H_RS25425 (ACEN9H_25430) - 5806724..5807425 (-) 702 WP_413672523.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEN9H_RS25430 (ACEN9H_25435) pilT 5807603..5808646 (+) 1044 WP_160409673.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEN9H_RS25435 (ACEN9H_25440) pilU 5808662..5809798 (+) 1137 WP_056122526.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEN9H_RS25440 (ACEN9H_25445) - 5809809..5810240 (-) 432 WP_413672524.1 hypothetical protein -
  ACEN9H_RS25445 (ACEN9H_25450) - 5810302..5810784 (+) 483 WP_371768748.1 glutathione peroxidase -
  ACEN9H_RS25450 (ACEN9H_25455) - 5810795..5811802 (-) 1008 WP_413672525.1 alpha/beta hydrolase family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38001.70 Da        Isoelectric Point: 6.7266

>NTDB_id=1044567 ACEN9H_RS25430 WP_160409673.1 5807603..5808646(+) (pilT) [Massilia cellulosiltytica strain CT11-92]
MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHGMIYDIMNDGQRKQYEEMLECDFSFAIPGLAR
FRVNAYNQERGASAVMRTIPSKILSLEELNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNENEYGHILTIED
PIEFVHDSKKCLINQREVGPHTLSFNNALRSALREDPDAILVGELRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRAMLSESLQAVISQTLLKTKDGAGRVAAHEIMIGTPAIRNLIREAKIAQMYSAIQTGSNVGMQTLDQN
LTDLVRRNVISSAAARAAAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1044567 ACEN9H_RS25430 WP_160409673.1 5807603..5808646(+) (pilT) [Massilia cellulosiltytica strain CT11-92]
ATGGACATCTCCGAACTGCTCGCTTTCTCGGTCAAGAACAAGGCCTCCGACCTGCACCTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGCATGGCGACGTACGCCGCATCAACCTGCCGCCGCTGGAGCACAAGGACGTGCACGGCATGATCTATGACA
TCATGAACGACGGCCAGCGCAAGCAGTACGAAGAGATGCTGGAGTGCGACTTCTCGTTCGCGATTCCCGGCCTCGCGCGC
TTCCGCGTCAACGCGTACAACCAGGAACGCGGTGCGTCCGCCGTGATGCGGACGATTCCGTCGAAGATCCTGTCGCTGGA
AGAACTGAACGCGCCGAAGATCTTCGCCGACCTCGCATTGAAGCCGCGCGGCCTCGTGCTCGTGACGGGTCCGACGGGCT
CCGGCAAGTCGACGACCCTCGCCGCGATGGTCAACCACCTGAACGAGAACGAGTACGGCCACATCCTCACCATCGAGGAT
CCGATCGAGTTCGTGCACGACTCGAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGTCCGCACACGCTGTCGTTCAACAA
CGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACGCGATCCTCGTCGGCGAGCTGCGCGACCTGGAGACGATCCGCCTCG
CGCTGTCCGCCGCCGAGACGGGCCACCTCGTGTTCGGCACCCTGCACACGTCGTCGGCCGCGAAGACCATCGACCGTATC
GTCGACGTGTTCCCGGCCGAGGAAAAGGAGATGGTGCGCGCGATGCTGTCGGAATCGCTGCAGGCCGTGATCTCGCAGAC
CCTGCTGAAAACGAAGGACGGCGCCGGCCGCGTGGCCGCGCACGAGATCATGATCGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAAGCGAAGATCGCGCAGATGTATTCCGCGATCCAGACCGGCTCCAACGTCGGCATGCAGACGCTGGACCAGAAC
CTGACGGACCTCGTGCGCCGCAACGTGATCTCGTCCGCGGCCGCGCGCGCCGCCGCCAAGATTCCCGAAAACTTCCCGGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.681

99.424

0.772
  pilT Acinetobacter baumannii D1279779

77.681

99.424

0.772
  pilT Acinetobacter baumannii strain A118

77.681

99.424

0.772
  pilT Pseudomonas aeruginosa PAK

77.101

99.424

0.767
  pilT Acinetobacter baylyi ADP1

75.072

99.424

0.746
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

74.926

97.695

0.732
  pilT Legionella pneumophila strain ERS1305867

74.926

97.695

0.732
  pilT Neisseria gonorrhoeae MS11

69.275

99.424

0.689
  pilT Neisseria meningitidis 8013

69.275

99.424

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.277

95.677

0.663
  pilT Vibrio cholerae strain A1552

69.277

95.677

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

99.424

0.516
  pilU Acinetobacter baylyi ADP1

41.908

99.712

0.418
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401
  pilU Vibrio cholerae strain A1552

41.003

97.695

0.401

Multiple sequence alignment