Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEN9H_RS25435 Genome accession   NZ_CP168562
Coordinates   5808662..5809798 (+) Length   378 a.a.
NCBI ID   WP_056122526.1    Uniprot ID   A0A7X3G1H3
Organism   Massilia cellulosiltytica strain CT11-92     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5803662..5814798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN9H_RS25405 (ACEN9H_25410) - 5803960..5804961 (+) 1002 WP_413672520.1 family 43 glycosylhydrolase -
  ACEN9H_RS25410 (ACEN9H_25415) - 5805031..5805408 (+) 378 WP_413672521.1 hypothetical protein -
  ACEN9H_RS25415 (ACEN9H_25420) - 5805405..5805896 (-) 492 WP_413672522.1 hypothetical protein -
  ACEN9H_RS25420 (ACEN9H_25425) proC 5805898..5806716 (-) 819 WP_160409671.1 pyrroline-5-carboxylate reductase -
  ACEN9H_RS25425 (ACEN9H_25430) - 5806724..5807425 (-) 702 WP_413672523.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEN9H_RS25430 (ACEN9H_25435) pilT 5807603..5808646 (+) 1044 WP_160409673.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEN9H_RS25435 (ACEN9H_25440) pilU 5808662..5809798 (+) 1137 WP_056122526.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEN9H_RS25440 (ACEN9H_25445) - 5809809..5810240 (-) 432 WP_413672524.1 hypothetical protein -
  ACEN9H_RS25445 (ACEN9H_25450) - 5810302..5810784 (+) 483 WP_371768748.1 glutathione peroxidase -
  ACEN9H_RS25450 (ACEN9H_25455) - 5810795..5811802 (-) 1008 WP_413672525.1 alpha/beta hydrolase family protein -
  ACEN9H_RS25455 (ACEN9H_25460) - 5811849..5813891 (-) 2043 WP_413672526.1 glycoside hydrolase family 97 protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41994.44 Da        Isoelectric Point: 6.4224

>NTDB_id=1044568 ACEN9H_RS25435 WP_056122526.1 5808662..5809798(+) (pilU) [Massilia cellulosiltytica strain CT11-92]
MERDQASKFMFDLLRLMVSKGGSDLFITSGFPPAMKIDGRMTPVSSQALTASHTADLARAIMNDKQAAGFELSKEANFAI
SPGDLGRFRVSAFMQMGAVGMVLRVITTTIPKFEDLDLPEVLKDVIMTKRGLVIMVGATGSGKSTTLATMVGYRNENSYG
HIITIEDPVEFVHPHKNCVITQREVGVDTDSFEAALKNSLRQAPDVIQIGEIRDRETMEHAIAFAETGHLCLATLHANSS
NQALDRIINFFPEERRQQLLMDLSLNMKGLISQRLIPKKEAKGRVVAMEIMLNSPLISDLIFKGEVHEIKEIMKKSRELG
MQTFDQALFDLYEADKITYEDALRNADSVNDLRLNIKLNSKHAKNRDFSAGTETLGLL

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1044568 ACEN9H_RS25435 WP_056122526.1 5808662..5809798(+) (pilU) [Massilia cellulosiltytica strain CT11-92]
ATGGAACGCGACCAGGCCTCCAAATTCATGTTCGACCTGCTGCGCCTGATGGTCAGCAAGGGCGGTTCCGACCTGTTCAT
CACGTCCGGCTTCCCGCCCGCGATGAAGATCGACGGCCGCATGACGCCCGTGTCCAGCCAGGCCCTCACGGCCTCGCACA
CGGCCGACCTGGCGCGCGCCATCATGAACGACAAGCAGGCCGCCGGCTTCGAGCTCTCGAAGGAAGCCAACTTCGCCATC
AGCCCCGGCGACCTGGGACGCTTCCGCGTCTCCGCCTTCATGCAGATGGGCGCCGTGGGCATGGTGCTGCGCGTGATCAC
GACGACGATCCCGAAGTTCGAGGACCTCGACCTGCCGGAGGTGCTGAAGGACGTGATCATGACCAAGCGCGGCCTCGTGA
TCATGGTCGGCGCCACGGGCTCCGGTAAATCGACGACGCTGGCGACGATGGTCGGCTACCGCAACGAGAACAGCTACGGC
CACATCATCACGATCGAGGATCCCGTCGAATTCGTCCACCCGCACAAGAACTGCGTGATCACGCAGCGCGAGGTCGGCGT
CGACACGGACAGCTTCGAGGCCGCGCTGAAGAACTCCCTGCGCCAGGCGCCGGACGTGATCCAGATCGGCGAAATTCGCG
ACCGCGAGACGATGGAACACGCGATCGCGTTCGCCGAGACGGGCCACCTGTGCCTGGCCACCCTGCACGCGAACAGCTCG
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCGGAGGAGCGCCGCCAGCAGCTGCTGATGGACCTGTCGCTGAACAT
GAAGGGACTGATCTCGCAGCGCCTGATTCCGAAGAAGGAAGCGAAGGGACGCGTGGTCGCGATGGAGATCATGCTGAACT
CGCCGCTGATCTCGGACCTGATCTTCAAGGGCGAGGTCCACGAGATCAAGGAGATCATGAAGAAGTCGCGCGAGCTGGGC
ATGCAGACGTTCGACCAGGCCCTGTTCGACCTGTACGAGGCGGACAAGATCACCTACGAGGATGCGCTGCGCAATGCGGA
CTCCGTGAACGACCTGCGCCTGAACATCAAGCTCAACAGCAAGCATGCGAAGAACCGCGATTTCTCCGCGGGCACCGAGA
CGCTCGGTCTCCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(101-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X3G1H3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

57.983

94.444

0.548
  pilU Vibrio cholerae strain A1552

56.18

94.18

0.529
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.776

88.624

0.397
  pilT Pseudomonas aeruginosa PAK

42.09

88.624

0.373
  pilT Acinetobacter nosocomialis M2

42.296

87.566

0.37
  pilT Pseudomonas stutzeri DSM 10701

41.493

88.624

0.368
  pilT Acinetobacter baumannii strain A118

43.218

83.862

0.362
  pilT Acinetobacter baumannii D1279779

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.492

83.333

0.362
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.492

83.333

0.362
  pilT Acinetobacter baylyi ADP1

42.812

84.656

0.362

Multiple sequence alignment