Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACEN9J_RS23300 Genome accession   NZ_CP168540
Coordinates   5037970..5039013 (-) Length   347 a.a.
NCBI ID   WP_057594012.1    Uniprot ID   A0A2E9BQD1
Organism   Variovorax sp. Varisp41 strain CT11-41     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5032970..5044013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN9J_RS23280 (ACEN9J_23280) - 5033618..5034526 (-) 909 WP_413762179.1 NAD(P)-dependent oxidoreductase -
  ACEN9J_RS23285 (ACEN9J_23285) - 5034600..5035115 (+) 516 WP_413762180.1 MerR family transcriptional regulator -
  ACEN9J_RS23290 (ACEN9J_23290) - 5035112..5036662 (+) 1551 WP_413762181.1 MFS transporter -
  ACEN9J_RS23295 (ACEN9J_23295) pilU 5036807..5037943 (-) 1137 WP_057594011.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEN9J_RS23300 (ACEN9J_23300) pilT 5037970..5039013 (-) 1044 WP_057594012.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEN9J_RS23305 (ACEN9J_23305) - 5039065..5039766 (+) 702 WP_057594013.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEN9J_RS23310 (ACEN9J_23310) - 5039777..5040244 (-) 468 WP_057594014.1 Lrp/AsnC ligand binding domain-containing protein -
  ACEN9J_RS23315 (ACEN9J_23315) - 5040356..5043331 (+) 2976 WP_413762182.1 L-glutamate gamma-semialdehyde dehydrogenase -
  ACEN9J_RS23320 (ACEN9J_23320) - 5043360..5043830 (-) 471 WP_093354401.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38250.02 Da        Isoelectric Point: 6.8885

>NTDB_id=1044524 ACEN9J_RS23300 WP_057594012.1 5037970..5039013(-) (pilT) [Variovorax sp. Varisp41 strain CT11-41]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKAVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMINYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMVRAMLSESLQAVISQTLCKTKDGMGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGQGMQTLDQN
LTDLVRRNTISAAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1044524 ACEN9J_RS23300 WP_057594012.1 5037970..5039013(-) (pilT) [Variovorax sp. Varisp41 strain CT11-41]
GTGGACATCACCCAACTGCTCGCGTTCAGCGTCAAGAACAAGGCCTCCGACCTGCACCTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTTCACGGCGATGTGCGCCGCATCAATGTCGATGCGCTGGACCACAAGGCGGTGCACGCCATGGTCTACGACA
TCATGAGCGACACCCACCGCAAGCACTACGAGGAGTTCCTGGAAGTCGACTTCTCGTTCGAGATCGACGGCCTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGGCGCGCGGGGCGGCCGCGGTGTTCCGGACCATCCCCTCGAAGATCCTCACGCTCGA
GCAGCTGAATGCGCCGAAGATTTTCGGCGAACTCGCGCTCAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCGACCACGCTGGCCGCGATGATCAACTACCTCAACGAGAACGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACGCTGTCGTTCTCGAA
CGCGCTGCGCTCGGCGCTGCGCGAGGACCCCGACGCCATCCTGGTCGGCGAAATGCGCGACCTCGAGACCATCCGCCTCG
CGATGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAGGAGAAGGAGATGGTCCGCGCGATGCTGTCGGAGTCGCTGCAGGCGGTGATCTCGCAGAC
CCTGTGCAAGACCAAGGACGGGATGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCCAGGGCATGCAGACGCTGGACCAGAAC
CTCACGGACCTGGTGCGGCGCAATACGATCTCCGCCGCGGAAGCCCGCAGCAAAGCGAAGATCCCCGAAAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2E9BQD1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria meningitidis 8013

68.406

99.424

0.68
  pilT Neisseria gonorrhoeae MS11

68.116

99.424

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.915

98.271

0.677
  pilT Vibrio cholerae strain A1552

68.915

98.271

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401

Multiple sequence alignment