Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEN9J_RS23295 Genome accession   NZ_CP168540
Coordinates   5036807..5037943 (-) Length   378 a.a.
NCBI ID   WP_057594011.1    Uniprot ID   -
Organism   Variovorax sp. Varisp41 strain CT11-41     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5031807..5042943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN9J_RS23270 (ACEN9J_23270) - 5031918..5032562 (+) 645 WP_057594006.1 SIS domain-containing protein -
  ACEN9J_RS23275 (ACEN9J_23275) - 5032790..5033545 (+) 756 WP_057594035.1 BON domain-containing protein -
  ACEN9J_RS23280 (ACEN9J_23280) - 5033618..5034526 (-) 909 WP_413762179.1 NAD(P)-dependent oxidoreductase -
  ACEN9J_RS23285 (ACEN9J_23285) - 5034600..5035115 (+) 516 WP_413762180.1 MerR family transcriptional regulator -
  ACEN9J_RS23290 (ACEN9J_23290) - 5035112..5036662 (+) 1551 WP_413762181.1 MFS transporter -
  ACEN9J_RS23295 (ACEN9J_23295) pilU 5036807..5037943 (-) 1137 WP_057594011.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEN9J_RS23300 (ACEN9J_23300) pilT 5037970..5039013 (-) 1044 WP_057594012.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEN9J_RS23305 (ACEN9J_23305) - 5039065..5039766 (+) 702 WP_057594013.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEN9J_RS23310 (ACEN9J_23310) - 5039777..5040244 (-) 468 WP_057594014.1 Lrp/AsnC ligand binding domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41809.93 Da        Isoelectric Point: 6.2427

>NTDB_id=1044523 ACEN9J_RS23295 WP_057594011.1 5036807..5037943(-) (pilU) [Variovorax sp. Varisp41 strain CT11-41]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSVMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDDLGMPQVLKDVSMTKRGLCILVGATGSGKSTTLAAMIDWRNENSFG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGLGRVAAVEILLNTPLISDLIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFEGHAISFEDALRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1044523 ACEN9J_RS23295 WP_057594011.1 5036807..5037943(-) (pilU) [Variovorax sp. Varisp41 strain CT11-41]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACGGCCGACTTCCCGCCCGCGATCAAGGTCGACGGCAAGGTCACCAAGGTCTCGCAGCAGGCGCTGGGCGCGCAGCACA
CGCTGGCGCTCACGCGCTCGGTGATGAACGACCGCCAGACGGCCGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCGACCGGCATCGGCCGCTTCCGCGTCAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATCCC
GGCCAAGCTGCCGACCATCGACGACCTGGGTATGCCGCAGGTGCTCAAGGACGTGTCGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCCGCGATGATCGACTGGCGCAACGAGAACTCCTTCGGC
CACATCGTCACGGTGGAGGACCCGGTGGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAGGTGGGCAT
CGACACCGACAGCTGGGAGGCGGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTCATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCACGCGGTGGCCTTCGCCGAGACCGGCCACCTCTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCACTCGACCGGATCATCAACTTCTTCCCCGAGGAGCGCCGCGCCCAGCTCCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGGTCTCGCAACGCCTGGTGCCGACCGAGGACGGCCTGGGCCGCGTGGCGGCGGTCGAGATCCTGCTGAACA
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTCGGCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGGGCCACGCGATCAGCTTCGAGGATGCGCTGCGCAACGCCGA
CTCGGCCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGACCTGGCCGCGGGCACGGAGC
ACTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

57.821

94.709

0.548
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Legionella pneumophila strain Lp02

44.073

87.037

0.384
  pilT Legionella pneumophila strain ERS1305867

44.073

87.037

0.384
  pilT Acinetobacter baylyi ADP1

42.342

88.095

0.373

Multiple sequence alignment