Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACEI37_RS24215 Genome accession   NZ_CP168303
Coordinates   2808072..2809115 (-) Length   347 a.a.
NCBI ID   WP_025583874.1    Uniprot ID   A0A375BRE3
Organism   Cupriavidus nantongensis strain HB4B5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2803072..2814115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEI37_RS24185 (ACEI37_24185) - 2804044..2804430 (+) 387 WP_062801674.1 CidA/LrgA family protein -
  ACEI37_RS24190 (ACEI37_24190) - 2804427..2805152 (+) 726 WP_115681079.1 LrgB family protein -
  ACEI37_RS24195 (ACEI37_24195) - 2805251..2805622 (+) 372 WP_181461224.1 VOC family protein -
  ACEI37_RS24200 (ACEI37_24200) - 2805645..2806343 (+) 699 WP_373378474.1 helix-turn-helix transcriptional regulator -
  ACEI37_RS24205 (ACEI37_24205) - 2806347..2806841 (-) 495 WP_111519207.1 glutathione peroxidase -
  ACEI37_RS24210 (ACEI37_24210) pilU 2806896..2808035 (-) 1140 WP_373378475.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEI37_RS24215 (ACEI37_24215) pilT 2808072..2809115 (-) 1044 WP_025583874.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEI37_RS24220 (ACEI37_24220) - 2809182..2809871 (+) 690 WP_373380309.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEI37_RS24225 (ACEI37_24225) proC 2809915..2810751 (+) 837 WP_373378476.1 pyrroline-5-carboxylate reductase -
  ACEI37_RS24230 (ACEI37_24230) ubiA 2810841..2811698 (-) 858 WP_373378477.1 4-hydroxybenzoate octaprenyltransferase -
  ACEI37_RS24235 (ACEI37_24235) - 2811840..2812325 (-) 486 WP_092306463.1 Dps family protein -
  ACEI37_RS24240 (ACEI37_24240) - 2812483..2813925 (-) 1443 WP_373378478.1 catalase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38394.18 Da        Isoelectric Point: 6.9772

>NTDB_id=1043782 ACEI37_RS24215 WP_025583874.1 2808072..2809115(-) (pilT) [Cupriavidus nantongensis strain HB4B5]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVASMTHKDVHAMVYDIMSDTQRKAYEERLEIDFSFEIAGLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLEELRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNENDMGHILTVED
PIEFVHSSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRSVISYNDARAIAKNPDAFMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1043782 ACEI37_RS24215 WP_025583874.1 2808072..2809115(-) (pilT) [Cupriavidus nantongensis strain HB4B5]
ATGGACATCGCGCAGCTATTGGCTTTCGCCGTCAAGAACAAGGCGTCCGATCTTCATCTGTCCGCGGACATGCCGCCGAT
GGTGCGGATCCACGGCGACATGCGGCGCATCAACGTCGCCTCGATGACGCACAAGGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACACCCAGCGCAAGGCCTACGAGGAACGCCTCGAAATCGATTTCTCGTTCGAGATCGCGGGCCTGTCGCGC
TTCCGCGTCAATGCCTATAACACCCAGCGCGGCGCCGCCGCGGTGTTCCGTACCATTCCGTCCAAGGTGCTGACGCTGGA
AGAGCTGCGCGCGCCGGCGGTGTTTGCCGACCTGTGCATGAAGCCGCGCGGGCTGGTGCTGGTGACGGGCCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGGTAGACCATCGCAATGAAAACGACATGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACAGCTCCAAGAAGAGTCTTATCAACCAGCGCGAGCTGGGGCCGCATACGCATTCGTTTGCCAA
CGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACGTGATCCTGGTGGGCGAACTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCGGCCGAAACCGGCCACCTGGTCTTCGGTACGCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCGTG
GTCGACGTGTTTCCGCCCGAAGAGAAGGACATGGTGCGCACCATGCTGTCGGAATCGCTCGAGGCGGTGATCTCGCAGAC
GCTGCTGAAGACCCGCGACGGCAACGGCCGCACCGCCGCGCACGAGATCATGATCGCCACGCCCGCGATCCGCCACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAGTGC
CTGTCGGACCTGATCAAGCGCAGCGTCATCAGCTACAACGACGCGCGCGCGATTGCCAAGAACCCGGACGCGTTCATGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A375BRE3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.965

99.712

0.718
  pilT Acinetobacter baumannii D1279779

71.965

99.712

0.718
  pilT Acinetobacter baumannii strain A118

71.965

99.712

0.718
  pilT Pseudomonas stutzeri DSM 10701

69.855

99.424

0.695
  pilT Pseudomonas aeruginosa PAK

69.855

99.424

0.695
  pilT Acinetobacter baylyi ADP1

69.565

99.424

0.692
  pilT Neisseria meningitidis 8013

67.246

99.424

0.669
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Legionella pneumophila strain Lp02

66.957

99.424

0.666
  pilT Legionella pneumophila strain ERS1305867

66.957

99.424

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.172

97.118

0.643
  pilT Vibrio cholerae strain A1552

66.172

97.118

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

99.424

0.493
  pilU Acinetobacter baylyi ADP1

40.116

99.135

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.542

0.392
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392

Multiple sequence alignment