Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEI37_RS24210 Genome accession   NZ_CP168303
Coordinates   2806896..2808035 (-) Length   379 a.a.
NCBI ID   WP_373378475.1    Uniprot ID   -
Organism   Cupriavidus nantongensis strain HB4B5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2801896..2813035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEI37_RS24180 (ACEI37_24180) glcF 2802707..2803960 (+) 1254 WP_062801673.1 glycolate oxidase subunit GlcF -
  ACEI37_RS24185 (ACEI37_24185) - 2804044..2804430 (+) 387 WP_062801674.1 CidA/LrgA family protein -
  ACEI37_RS24190 (ACEI37_24190) - 2804427..2805152 (+) 726 WP_115681079.1 LrgB family protein -
  ACEI37_RS24195 (ACEI37_24195) - 2805251..2805622 (+) 372 WP_181461224.1 VOC family protein -
  ACEI37_RS24200 (ACEI37_24200) - 2805645..2806343 (+) 699 WP_373378474.1 helix-turn-helix transcriptional regulator -
  ACEI37_RS24205 (ACEI37_24205) - 2806347..2806841 (-) 495 WP_111519207.1 glutathione peroxidase -
  ACEI37_RS24210 (ACEI37_24210) pilU 2806896..2808035 (-) 1140 WP_373378475.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEI37_RS24215 (ACEI37_24215) pilT 2808072..2809115 (-) 1044 WP_025583874.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEI37_RS24220 (ACEI37_24220) - 2809182..2809871 (+) 690 WP_373380309.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEI37_RS24225 (ACEI37_24225) proC 2809915..2810751 (+) 837 WP_373378476.1 pyrroline-5-carboxylate reductase -
  ACEI37_RS24230 (ACEI37_24230) ubiA 2810841..2811698 (-) 858 WP_373378477.1 4-hydroxybenzoate octaprenyltransferase -
  ACEI37_RS24235 (ACEI37_24235) - 2811840..2812325 (-) 486 WP_092306463.1 Dps family protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42206.43 Da        Isoelectric Point: 6.6918

>NTDB_id=1043781 ACEI37_RS24210 WP_373378475.1 2806896..2808035(-) (pilU) [Cupriavidus nantongensis strain HB4B5]
MLDRESAAKYINDLLELMVSNRGSDLFITSDFPPAIKVDGKITPVSQQPLNPTQALGLVRSVMNERQVQDFDTSRECNFA
ISVPKAGRFRVSAFIQQGKAGMVVRTINTRIPSVADLDLPQTLHDVVMAKRGLVIVTGATGSGKSTTLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRVVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFDLYEQGKITYEDALRNADSLNDLRLMIKLHSTHAKDSDLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1043781 ACEI37_RS24210 WP_373378475.1 2806896..2808035(-) (pilU) [Cupriavidus nantongensis strain HB4B5]
ATGCTCGACCGCGAATCCGCCGCCAAGTACATCAACGACCTGCTCGAGCTGATGGTCAGCAACCGCGGCTCGGACCTGTT
CATCACTTCGGACTTCCCGCCGGCGATCAAGGTCGACGGCAAGATCACGCCGGTGTCGCAGCAGCCGCTGAACCCGACCC
AGGCGCTGGGGCTGGTGCGCTCGGTGATGAACGAGCGCCAGGTGCAGGACTTCGACACCAGCCGCGAATGCAACTTTGCC
ATCAGCGTGCCCAAGGCCGGGCGCTTCCGCGTCTCGGCCTTTATCCAGCAGGGCAAGGCCGGCATGGTGGTGCGTACCAT
CAATACGCGCATCCCGTCGGTGGCCGACCTGGACCTGCCGCAGACGCTGCATGACGTGGTCATGGCCAAGCGCGGGCTGG
TGATCGTCACCGGTGCAACCGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGCTGGACCACCGCAACGCGCACTCCTAC
GGCCACATCATCACCATCGAGGATCCGATCGAGTACGTGCACGCGCACCAGAACTGCATCGTCACGCAGCGTGAGGTCGG
CATCGATACCGAGTCGTGGCACGTGGCGCTGAAGAACACGCTGCGCCAGGCCCCCGACGTGATCCTGATCGGCGAGATCC
GCGATCGCGAGACCATGGAGTACGCGATGCAGTACGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAAC
GCCAACCAGGCGATCGACCGCGTGGTCAACTTCTTCCCCGAGGAAAAGCGCCAGCAACTGCTGATCGACCTGTCGCTGAA
CCTGAAGGCGATGATCTCGCAGCGCCTGCTGCCGCGCGCCGGCCGCAAGGGCCGGGTGCCGGCGGTGGAGATCATGATCG
GCACGCCGCTGGTGGCCGATCTGATCTTCAAGGGCGAGATCCACGAGCTCAAGGAAGTCATCAAGAAGTCGCGCGAGCAG
GGCATGATCTCGTTCGACCAGGCGTTGTTCGACCTGTACGAGCAGGGCAAGATCACCTACGAGGACGCGCTGCGCAATGC
CGACTCGCTCAACGACCTGCGCCTGATGATCAAGCTGCACAGCACCCATGCGAAGGACTCGGACCTGGGTGCCGGCACCG
AGCATCTCAACGTCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.218

91.821

0.58
  pilU Acinetobacter baylyi ADP1

57.714

92.348

0.533
  pilU Vibrio cholerae strain A1552

53.719

95.778

0.515
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

88.918

0.404
  pilT Pseudomonas stutzeri DSM 10701

43.62

88.918

0.388
  pilT Pseudomonas aeruginosa PAK

43.62

88.918

0.388
  pilT Acinetobacter baumannii D1279779

43.844

87.863

0.385
  pilT Acinetobacter baylyi ADP1

43.068

89.446

0.385
  pilT Acinetobacter nosocomialis M2

43.844

87.863

0.385
  pilT Acinetobacter baumannii strain A118

43.844

87.863

0.385
  pilT Legionella pneumophila strain Lp02

42.042

87.863

0.369
  pilT Legionella pneumophila strain ERS1305867

42.042

87.863

0.369
  pilT Neisseria meningitidis 8013

40.588

89.71

0.364

Multiple sequence alignment