Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABNX08_RS18080 Genome accession   NZ_CP168265
Coordinates   3869270..3870313 (-) Length   347 a.a.
NCBI ID   WP_022536528.1    Uniprot ID   -
Organism   Cupriavidus sp. DF5525 strain 73-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3864270..3875313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNX08_RS18050 (ABNX08_018050) - 3865237..3865608 (+) 372 WP_022536522.1 CidA/LrgA family protein -
  ABNX08_RS18055 (ABNX08_018055) - 3865605..3866330 (+) 726 WP_349606316.1 LrgB family protein -
  ABNX08_RS18060 (ABNX08_018060) - 3866415..3866786 (+) 372 WP_022536524.1 VOC family protein -
  ABNX08_RS18065 (ABNX08_018065) - 3866808..3867497 (+) 690 WP_022536525.1 YafY family protein -
  ABNX08_RS18070 (ABNX08_018070) - 3867505..3867999 (-) 495 WP_022536526.1 glutathione peroxidase -
  ABNX08_RS18075 (ABNX08_018075) pilU 3868066..3869205 (-) 1140 WP_022536527.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABNX08_RS18080 (ABNX08_018080) pilT 3869270..3870313 (-) 1044 WP_022536528.1 type IV pilus twitching motility protein PilT Machinery gene
  ABNX08_RS18085 (ABNX08_018085) - 3870381..3871070 (+) 690 WP_022536529.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABNX08_RS18090 (ABNX08_018090) proC 3871105..3871941 (+) 837 WP_022536530.1 pyrroline-5-carboxylate reductase -
  ABNX08_RS18095 (ABNX08_018095) ubiA 3872077..3872934 (-) 858 WP_041681151.1 4-hydroxybenzoate octaprenyltransferase -
  ABNX08_RS18100 (ABNX08_018100) - 3873087..3873572 (-) 486 WP_022536532.1 Dps family protein -
  ABNX08_RS18105 (ABNX08_018105) - 3873666..3873863 (-) 198 WP_041681152.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38421.20 Da        Isoelectric Point: 6.9771

>NTDB_id=1043562 ABNX08_RS18080 WP_022536528.1 3869270..3870313(-) (pilT) [Cupriavidus sp. DF5525 strain 73-1]
MDIAQLLAFAVKNKASDLHLSSDMPPMVRIHGDMRRINVASMAHKDVHAMVYDIMSDTQRKIYEERLEIDFSFEIAGLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLEELRAPAVFGDLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNDNDMGHILTVED
PIEFVHNSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRSVISYNDARAIAKNPDAFMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1043562 ABNX08_RS18080 WP_022536528.1 3869270..3870313(-) (pilT) [Cupriavidus sp. DF5525 strain 73-1]
ATGGACATCGCGCAGCTATTGGCTTTCGCCGTCAAGAACAAGGCGTCCGATCTTCATCTCTCGTCGGACATGCCGCCGAT
GGTGCGGATCCACGGCGATATGCGCCGGATCAACGTCGCATCGATGGCGCACAAGGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACACCCAGCGCAAGATCTACGAAGAACGGCTCGAGATCGACTTCTCGTTCGAGATCGCCGGCCTGTCGCGC
TTCCGGGTCAACGCCTACAACACGCAGCGCGGCGCCGCCGCGGTGTTCCGTACGATTCCCTCCAAGGTCCTGACGCTCGA
GGAGCTGCGCGCACCGGCGGTGTTCGGCGACCTGTGCATGAAGCCGCGCGGGCTGGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGGTCGACCATCGCAATGATAACGACATGGGTCACATCCTGACGGTGGAAGAC
CCGATCGAATTCGTCCACAACTCAAAGAAGAGCCTGATCAACCAGCGCGAGCTGGGCCCGCACACGCATTCCTTTGCCAA
CGCGCTGCGCTCGGCACTGCGTGAAGACCCGGACGTGATCCTGGTGGGCGAACTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTCTTCGGCACGCTGCACACCAGCTCCGCGGCCAAGACCATCGACCGTGTG
GTCGATGTGTTCCCGCCGGAAGAAAAGGACATGGTGCGCACCATGCTGTCGGAATCGCTGGAGGCGGTGATCTCGCAGAC
GCTGCTGAAGACCCGCGATGGCAACGGCCGTACCGCCGCGCACGAGATCATGATCGCCACGCCCGCGATCCGCCACTTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAGTGC
CTGTCGGACCTGATCAAGCGCAGCGTCATCAGCTACAACGACGCGCGCGCCATCGCCAAGAATCCGGACGCGTTCATGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

71.387

99.712

0.712
  pilT Acinetobacter baumannii strain A118

71.387

99.712

0.712
  pilT Acinetobacter nosocomialis M2

71.387

99.712

0.712
  pilT Pseudomonas stutzeri DSM 10701

70.145

99.424

0.697
  pilT Pseudomonas aeruginosa PAK

69.565

99.424

0.692
  pilT Acinetobacter baylyi ADP1

69.275

99.424

0.689
  pilT Neisseria gonorrhoeae MS11

67.536

99.424

0.671
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Legionella pneumophila strain Lp02

66.667

99.424

0.663
  pilT Legionella pneumophila strain ERS1305867

66.667

99.424

0.663
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.282

97.118

0.634
  pilT Vibrio cholerae strain A1552

65.282

97.118

0.634
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

99.424

0.493
  pilU Acinetobacter baylyi ADP1

39.826

99.135

0.395
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392
  pilU Pseudomonas stutzeri DSM 10701

40.299

96.542

0.389

Multiple sequence alignment