Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABNX08_RS18075 Genome accession   NZ_CP168265
Coordinates   3868066..3869205 (-) Length   379 a.a.
NCBI ID   WP_022536527.1    Uniprot ID   -
Organism   Cupriavidus sp. DF5525 strain 73-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3863066..3874205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNX08_RS18045 (ABNX08_018045) glcF 3863893..3865146 (+) 1254 WP_022536521.1 glycolate oxidase subunit GlcF -
  ABNX08_RS18050 (ABNX08_018050) - 3865237..3865608 (+) 372 WP_022536522.1 CidA/LrgA family protein -
  ABNX08_RS18055 (ABNX08_018055) - 3865605..3866330 (+) 726 WP_349606316.1 LrgB family protein -
  ABNX08_RS18060 (ABNX08_018060) - 3866415..3866786 (+) 372 WP_022536524.1 VOC family protein -
  ABNX08_RS18065 (ABNX08_018065) - 3866808..3867497 (+) 690 WP_022536525.1 YafY family protein -
  ABNX08_RS18070 (ABNX08_018070) - 3867505..3867999 (-) 495 WP_022536526.1 glutathione peroxidase -
  ABNX08_RS18075 (ABNX08_018075) pilU 3868066..3869205 (-) 1140 WP_022536527.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABNX08_RS18080 (ABNX08_018080) pilT 3869270..3870313 (-) 1044 WP_022536528.1 type IV pilus twitching motility protein PilT Machinery gene
  ABNX08_RS18085 (ABNX08_018085) - 3870381..3871070 (+) 690 WP_022536529.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABNX08_RS18090 (ABNX08_018090) proC 3871105..3871941 (+) 837 WP_022536530.1 pyrroline-5-carboxylate reductase -
  ABNX08_RS18095 (ABNX08_018095) ubiA 3872077..3872934 (-) 858 WP_041681151.1 4-hydroxybenzoate octaprenyltransferase -
  ABNX08_RS18100 (ABNX08_018100) - 3873087..3873572 (-) 486 WP_022536532.1 Dps family protein -
  ABNX08_RS18105 (ABNX08_018105) - 3873666..3873863 (-) 198 WP_041681152.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42284.55 Da        Isoelectric Point: 6.8262

>NTDB_id=1043561 ABNX08_RS18075 WP_022536527.1 3868066..3869205(-) (pilU) [Cupriavidus sp. DF5525 strain 73-1]
MLDRESASKYINDLLELMVSNRGSDLFITSDFPPAIKVDGKITPVSQQPLNPTQALGLVRSVMNERQVQDFDTSRECNFA
ITAPKAGRFRVSAFIQQGKAGMVVRTINTRIPSVADLDLPPTLHEIVMSKRGLVIVTGATGSGKSTTLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRVVNFFPEEKRQQLLIDLSLNLKAMVSQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFELHEEGKITYEDALRNADSLNDLRLMIKLHSKRARDTDLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1043561 ABNX08_RS18075 WP_022536527.1 3868066..3869205(-) (pilU) [Cupriavidus sp. DF5525 strain 73-1]
ATGCTCGATCGCGAATCCGCTTCCAAGTACATCAACGACCTGCTGGAGCTGATGGTCAGCAACCGCGGCTCGGACCTGTT
CATCACCTCGGACTTCCCGCCGGCGATCAAGGTCGACGGCAAGATCACGCCGGTGTCGCAGCAGCCGCTGAACCCGACAC
AGGCGCTGGGCCTGGTGCGCTCGGTGATGAACGAGCGCCAGGTGCAGGACTTCGACACCAGCCGCGAATGCAATTTCGCC
ATCACCGCGCCCAAGGCCGGGCGTTTCCGCGTGTCGGCGTTTATCCAGCAGGGCAAGGCCGGCATGGTGGTGCGTACCAT
CAACACGCGCATCCCCTCGGTGGCGGACCTGGACCTGCCGCCGACGCTGCATGAGATCGTGATGTCCAAGCGCGGGCTGG
TGATCGTCACCGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGCTGGACCATCGCAACGCGCACTCGTAC
GGCCACATCATCACCATCGAGGACCCAATCGAGTACGTGCATGCGCACCAGAACTGCATCGTCACGCAGCGCGAGGTCGG
CATCGATACCGAGTCCTGGCATGTGGCGCTGAAAAACACGCTGCGGCAGGCGCCTGACGTGATCCTGATCGGCGAAATCC
GCGACCGCGAGACCATGGAGTACGCGATGCAGTACGCGGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAAC
GCCAACCAGGCGATCGACCGCGTGGTCAACTTCTTCCCCGAGGAAAAGCGCCAGCAGTTGCTGATCGACCTGTCGCTGAA
CCTGAAGGCGATGGTCTCGCAGCGCCTGTTGCCGCGCGCCGGCCGCAAGGGCCGTGTGCCGGCGGTGGAAATCATGATCG
GCACGCCGCTGGTGGCCGATCTGATCTTCAAGGGCGAGATCCACGAGCTCAAGGAAGTCATCAAGAAATCGCGCGAGCAG
GGCATGATCTCGTTCGACCAGGCGCTCTTCGAGCTGCACGAGGAAGGCAAGATCACTTACGAAGACGCGCTGCGCAACGC
GGACTCGCTCAACGACCTGCGCCTGATGATCAAGCTGCACAGCAAGCGCGCCCGGGATACGGACCTGGGTGCCGGTACCG
AGCACCTCAACGTCATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(54-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.751

92.084

0.578
  pilU Acinetobacter baylyi ADP1

57.714

92.348

0.533
  pilU Vibrio cholerae strain A1552

53.168

95.778

0.509
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

88.918

0.398
  pilT Pseudomonas stutzeri DSM 10701

43.323

88.918

0.385
  pilT Pseudomonas aeruginosa PAK

42.73

88.918

0.38
  pilT Acinetobacter nosocomialis M2

42.943

87.863

0.377
  pilT Acinetobacter baumannii D1279779

42.943

87.863

0.377
  pilT Acinetobacter baumannii strain A118

42.943

87.863

0.377
  pilT Acinetobacter baylyi ADP1

42.643

87.863

0.375
  pilT Legionella pneumophila strain ERS1305867

44.099

84.96

0.375
  pilT Legionella pneumophila strain Lp02

44.099

84.96

0.375
  pilT Neisseria meningitidis 8013

40.588

89.71

0.364

Multiple sequence alignment