Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACEN3N_RS04745 Genome accession   NZ_CP168228
Coordinates   1019190..1020227 (-) Length   345 a.a.
NCBI ID   WP_005030845.1    Uniprot ID   A0A0A8TTJ0
Organism   Acinetobacter bereziniae strain 2024CK-01176     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1014190..1025227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN3N_RS04715 (ACEN3N_04715) - 1014860..1015315 (+) 456 WP_004828043.1 bacteriohemerythrin -
  ACEN3N_RS04720 (ACEN3N_04720) - 1015516..1016598 (+) 1083 WP_034708196.1 membrane protein -
  ACEN3N_RS04725 (ACEN3N_04725) - 1016616..1016939 (+) 324 WP_010591161.1 RnfH family protein -
  ACEN3N_RS04730 (ACEN3N_04730) - 1017021..1017419 (-) 399 WP_004828048.1 outer membrane protein assembly factor BamE -
  ACEN3N_RS04735 (ACEN3N_04735) fur 1017532..1017969 (+) 438 WP_004828050.1 ferric iron uptake transcriptional regulator -
  ACEN3N_RS04740 (ACEN3N_04740) pilU 1018044..1019162 (-) 1119 WP_004828052.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEN3N_RS04745 (ACEN3N_04745) pilT 1019190..1020227 (-) 1038 WP_005030845.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEN3N_RS04750 (ACEN3N_04750) - 1020674..1021366 (+) 693 WP_373350811.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEN3N_RS04755 (ACEN3N_04755) - 1021491..1022324 (+) 834 WP_004828057.1 undecaprenyl-diphosphate phosphatase -
  ACEN3N_RS04760 (ACEN3N_04760) - 1022502..1023287 (+) 786 WP_288497341.1 hypothetical protein -
  ACEN3N_RS04765 (ACEN3N_04765) - 1023518..1024273 (+) 756 WP_004828060.1 hypothetical protein -
  ACEN3N_RS04770 (ACEN3N_04770) - 1024365..1024763 (+) 399 WP_373350812.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38254.96 Da        Isoelectric Point: 6.5055

>NTDB_id=1043270 ACEN3N_RS04745 WP_005030845.1 1019190..1020227(-) (pilT) [Acinetobacter bereziniae strain 2024CK-01176]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHRLVYDIMNDKQRRDFEEQLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDLCDLPRGLILVTGPTGSGKSTTLASMIDFINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KALVAKGVISPQVARNVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1043270 ACEN3N_RS04745 WP_005030845.1 1019190..1020227(-) (pilT) [Acinetobacter bereziniae strain 2024CK-01176]
ATGGATATTACAGAATTATTGGCGTTTTCCGTTAAAAATGGTGCGTCTGACTTACACCTTTCAGCGGGTATGCCACCGAT
GATTCGTGTTGATGGTGAAGTCCGCCGTATCAATTTACCAGCATTGGAACATAAAGAAGTTCATAGACTGGTCTATGACA
TCATGAATGACAAACAACGTCGAGATTTTGAAGAACAATTAGAGACCGACTTCTCTTTCGAAGTTCCAGGCGTTGCCCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCAGGTGCGGTATTCCGTACCATTCCATCCAAAGTCCTGACTATGGA
GGATCTTGGTTTAGGACAAATCTTTAAAGACCTTTGTGACTTACCACGCGGTTTAATTTTGGTTACAGGTCCAACAGGTT
CTGGTAAATCAACCACTTTGGCTTCGATGATTGACTTCATCAATGACAATCGTTATGACCATATTTTAACTGTAGAAGAT
CCAATCGAATTTGTACACCAATCTAAAAAGTGCTTGATCAACCAGCGTGAAGTGCATCGTGATACACATGGCTTTAACGA
GGCGCTACGTTCTGCACTGCGTGAAGATCCAGACATTATCCTTGTCGGTGAGATGCGTGACCTTGAAACTATCCGCCTTG
CACTTACCGCAGCAGAAACAGGTCACTTGGTCTTTGGTACACTGCATACCACTTCCGCAGCTAAGACCATTGACCGTGTG
ATTGATGTATTCCCTGCCGAAGAAAAAGACATGGTGCGTGCCATGCTTTCTGAATCATTACAAGCTGTTATTTCTCAAAC
TCTATTAAAGAAAAATGGCGGTGGACGTGTTGCTGCACATGAAATCATGATCGGTATTCCTGCAATTCGTAACCTGATTC
GTGAAAACAAAGTTGCACAGATGTATTCAGCAATTCAGACTGGTGCAAACTATGGTATGACCACACTGGATCAAAGCTTA
AAAGCGCTGGTTGCCAAAGGTGTAATTAGTCCTCAAGTTGCGCGAAATGTCGCAAAACAACCAGAATCATTTCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A8TTJ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

94.783

100

0.948
  pilT Acinetobacter baumannii D1279779

94.783

100

0.948
  pilT Acinetobacter nosocomialis M2

94.493

100

0.945
  pilT Acinetobacter baylyi ADP1

86.919

99.71

0.867
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Neisseria meningitidis 8013

66.957

100

0.67
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.578

96.232

0.67
  pilT Vibrio cholerae strain A1552

69.578

96.232

0.67
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.664
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.516
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Acinetobacter baylyi ADP1

39.884

100

0.4
  pilU Pseudomonas stutzeri DSM 10701

41.39

95.942

0.397

Multiple sequence alignment