Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEN3N_RS04740 Genome accession   NZ_CP168228
Coordinates   1018044..1019162 (-) Length   372 a.a.
NCBI ID   WP_004828052.1    Uniprot ID   -
Organism   Acinetobacter bereziniae strain 2024CK-01176     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1013044..1024162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN3N_RS04710 (ACEN3N_04710) - 1013727..1014575 (+) 849 WP_009585828.1 class II glutamine amidotransferase -
  ACEN3N_RS04715 (ACEN3N_04715) - 1014860..1015315 (+) 456 WP_004828043.1 bacteriohemerythrin -
  ACEN3N_RS04720 (ACEN3N_04720) - 1015516..1016598 (+) 1083 WP_034708196.1 membrane protein -
  ACEN3N_RS04725 (ACEN3N_04725) - 1016616..1016939 (+) 324 WP_010591161.1 RnfH family protein -
  ACEN3N_RS04730 (ACEN3N_04730) - 1017021..1017419 (-) 399 WP_004828048.1 outer membrane protein assembly factor BamE -
  ACEN3N_RS04735 (ACEN3N_04735) fur 1017532..1017969 (+) 438 WP_004828050.1 ferric iron uptake transcriptional regulator -
  ACEN3N_RS04740 (ACEN3N_04740) pilU 1018044..1019162 (-) 1119 WP_004828052.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEN3N_RS04745 (ACEN3N_04745) pilT 1019190..1020227 (-) 1038 WP_005030845.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEN3N_RS04750 (ACEN3N_04750) - 1020674..1021366 (+) 693 WP_373350811.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEN3N_RS04755 (ACEN3N_04755) - 1021491..1022324 (+) 834 WP_004828057.1 undecaprenyl-diphosphate phosphatase -
  ACEN3N_RS04760 (ACEN3N_04760) - 1022502..1023287 (+) 786 WP_288497341.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41853.20 Da        Isoelectric Point: 6.4218

>NTDB_id=1043269 ACEN3N_RS04740 WP_004828052.1 1018044..1019162(-) (pilU) [Acinetobacter bereziniae strain 2024CK-01176]
MDFNDLLNMMIEQKASDLFITADVEPSMKINGQIVPVVKTKLNGEIIMQLLNSIMSEKQRKEFADTRECNFAITNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPTMEELKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFESDRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLVSDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLQIKLDEEGGAQLLNANSKLTFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1043269 ACEN3N_RS04740 WP_004828052.1 1018044..1019162(-) (pilU) [Acinetobacter bereziniae strain 2024CK-01176]
ATGGACTTTAATGACTTGCTCAATATGATGATTGAGCAGAAAGCATCCGACTTGTTTATTACCGCAGATGTAGAACCATC
AATGAAAATTAATGGTCAAATCGTCCCTGTGGTAAAAACCAAGTTAAACGGCGAAATCATCATGCAATTGCTCAACTCAA
TCATGAGTGAGAAGCAACGCAAAGAATTTGCAGATACGCGCGAATGTAACTTTGCCATTACCAACAGAGATAAAACTGCC
CGTTTCCGTGTCAGCGCTTTTCAACAACGTGATATGCCAGGTATGGTTTTGCGTCGTATCGAAACTAAAATACCAACCAT
GGAAGAGTTAAAACTTCCACCTATACTCAAAGATCTAGCCATGACAAAACGTGGCATCATTATCTTTGTTGGTGCGACAG
GTACCGGTAAATCCACATCTCTTGCATCGATTATTGGCTATCGCAATCAGAACTCAAAAGGTCATATCATCACCATTGAA
GATCCGATTGAATTTGTTCACGAACATGCTGGCTGTATCATCACCCAACGTGAAGTTGGTATTGATACTGACTCTTTTGA
AATTGCCTTGAAGAACACCTTGCGTCAAGCACCTGATGTTATTCTGATTGGTGAGATTCGTTCCCGTGAAACAATGGATT
ATGCGATTGCATTTGCTGAAACTGGACATCTGGTCTTTGCGACACTACATGCCAACAATGCCAACCAAGCCATTGACCGC
ATTATTCACTTCTTTGAATCTGACCGTCATAGCCAATTGTTTATGGACTTATCTCTTAACCTTAAAGCTATGGTCGCACA
GCAATTAATTCCAACACCAGATGGTAACTCGCGTCGTGCAGCGATTGAGATTCTGATCAACTCACCACTCGTTTCAGATC
TCATCCGTAAAGGTGATGTACATGAAATCAAAGATTTGATGAAACGTTCACGCGAATTGGGTATGCAAACTTTTGACCAA
GCCTTGTTTGACCTTTATAAAGCTGGTCAGATTACCTACAAAGATGCGTTGAAACATGCAGACTCACCAAATGATTTACG
TCTACAAATCAAATTGGATGAAGAAGGTGGCGCGCAGTTATTGAATGCCAACAGCAAATTGACTTTTGATGGGCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.19

100

0.774
  pilU Pseudomonas stutzeri DSM 10701

68.464

99.731

0.683
  pilU Vibrio cholerae strain A1552

54.865

99.462

0.546
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.064

93.011

0.401
  pilT Legionella pneumophila strain ERS1305867

40.828

90.86

0.371
  pilT Legionella pneumophila strain Lp02

40.828

90.86

0.371
  pilT Acinetobacter baumannii D1279779

39.942

92.204

0.368
  pilT Acinetobacter baumannii strain A118

39.942

92.204

0.368
  pilT Acinetobacter nosocomialis M2

39.942

92.204

0.368
  pilT Pseudomonas aeruginosa PAK

39.595

93.011

0.368
  pilT Acinetobacter baylyi ADP1

39.359

92.204

0.363
  pilT Neisseria meningitidis 8013

39.706

91.398

0.363
  pilT Neisseria gonorrhoeae MS11

39.706

91.398

0.363
  pilT Pseudomonas stutzeri DSM 10701

38.728

93.011

0.36

Multiple sequence alignment