Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACEL84_RS02160 Genome accession   NZ_CP168212
Coordinates   454521..455558 (+) Length   345 a.a.
NCBI ID   WP_261894724.1    Uniprot ID   -
Organism   Vibrio porteresiae strain MSSRF31     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 449521..460558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEL84_RS02130 (ACEL84_02130) hemW 450052..451230 (-) 1179 WP_261894716.1 radical SAM family heme chaperone HemW -
  ACEL84_RS02135 (ACEL84_02135) - 451230..451829 (-) 600 WP_261894717.1 XTP/dITP diphosphatase -
  ACEL84_RS02140 (ACEL84_02140) yggU 451996..452286 (-) 291 WP_261894718.1 DUF167 family protein YggU -
  ACEL84_RS02145 (ACEL84_02145) - 452286..452843 (-) 558 WP_261894720.1 YggT family protein -
  ACEL84_RS02150 (ACEL84_02150) proC 452916..453734 (-) 819 WP_261894721.1 pyrroline-5-carboxylate reductase -
  ACEL84_RS02155 (ACEL84_02155) - 453788..454495 (-) 708 WP_261894722.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEL84_RS02160 (ACEL84_02160) pilT 454521..455558 (+) 1038 WP_261894724.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEL84_RS02165 (ACEL84_02165) pilU 455569..456675 (+) 1107 WP_261894725.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEL84_RS02170 (ACEL84_02170) ruvX 456723..457145 (-) 423 WP_261894726.1 Holliday junction resolvase RuvX -
  ACEL84_RS02175 (ACEL84_02175) - 457221..457784 (-) 564 WP_261896206.1 YqgE/AlgH family protein -
  ACEL84_RS02180 (ACEL84_02180) gshB 457825..458781 (-) 957 WP_261894727.1 glutathione synthase -
  ACEL84_RS02185 (ACEL84_02185) rsmE 458795..459526 (-) 732 WP_261894728.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACEL84_RS02190 (ACEL84_02190) - 459615..460319 (-) 705 WP_261894729.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38142.68 Da        Isoelectric Point: 6.6208

>NTDB_id=1043094 ACEL84_RS02160 WP_261894724.1 454521..455558(+) (pilT) [Vibrio porteresiae strain MSSRF31]
MDIAELLDFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFSHTEVHRLVFEIMNDSQRREYEENLEVDFSFELPNIGR
FRVNAFHQSRGGSAVFRTIPTEVPTLAQLNAPEIFHKMTQYEKGLVLVTGPTGSGKSTTLAAMINEINATQNKHILTIED
PIEFVHANNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGADKGMVRSMLSESLRAVIAQKLLKKVGGGRVACHEVMLGTPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMSKGLVELSEVQSKIESDMANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1043094 ACEL84_RS02160 WP_261894724.1 454521..455558(+) (pilT) [Vibrio porteresiae strain MSSRF31]
ATGGATATCGCCGAATTACTCGATTTTAGTGTAAAGCATAATGCCTCTGATCTACACCTTTCTGCTGGTGTACCTCCGAT
GGTCAGAATTGATGGCGAAGTAAGAAAACTTGGCGTTCCTGCGTTTTCTCATACGGAAGTTCACCGTTTGGTCTTTGAGA
TCATGAATGATTCCCAAAGACGTGAGTATGAAGAGAATCTCGAAGTCGATTTCTCTTTTGAACTTCCCAATATTGGTCGT
TTTCGTGTTAATGCTTTTCATCAATCTCGAGGTGGTTCCGCTGTTTTTCGGACCATTCCTACCGAAGTCCCTACCTTAGC
ACAATTAAATGCCCCTGAGATCTTTCATAAAATGACTCAGTATGAAAAAGGCTTAGTCTTGGTTACCGGTCCAACGGGGT
CTGGTAAATCAACTACCTTGGCGGCAATGATTAACGAGATTAATGCGACGCAGAATAAGCATATTTTGACGATTGAAGAC
CCAATTGAATTTGTTCACGCCAATAACAAATGCTTGATTAACCAACGTGAAGTTCATCGTGATACACACAGTTTTAAAGC
TGCATTGCGTTCGGCGTTACGTGAAGATCCGGATGTGATTTTGGTCGGTGAGCTGCGTGACCAAGAGACCATCAGCTTGG
CTTTGACTGCGGCGGAAACGGGACACTTGGTTTTTGGTACTTTGCATACCAGCTCAGCAGCGAAAACCATCGATAGAATT
ATTGACGTATTTCCGGGGGCGGATAAAGGCATGGTGCGTTCCATGTTGTCGGAATCGTTACGCGCAGTTATTGCGCAAAA
ATTATTAAAAAAGGTCGGTGGTGGTCGAGTGGCTTGCCATGAAGTTATGTTAGGCACTCCTGCTATTCGTAATCTGATTC
GAGAAGATAAAGTCGCACAGATGTACTCCATTATTCAAACCGGTGCCGCGCACGGGATGCAAACAATGGAGCAAAATGCC
CGACAATTAATGTCAAAAGGCCTCGTTGAGTTATCCGAAGTTCAAAGCAAAATCGAGTCAGATATGGCTAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

87.536

100

0.875
  pilT Vibrio cholerae strain A1552

87.536

100

0.875
  pilT Acinetobacter baylyi ADP1

69.706

98.551

0.687
  pilT Acinetobacter nosocomialis M2

71.429

95.362

0.681
  pilT Acinetobacter baumannii strain A118

71.125

95.362

0.678
  pilT Acinetobacter baumannii D1279779

71.125

95.362

0.678
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

67.062

97.681

0.655
  pilT Legionella pneumophila strain Lp02

67.781

95.362

0.646
  pilT Legionella pneumophila strain ERS1305867

67.781

95.362

0.646
  pilT Neisseria meningitidis 8013

65.152

95.652

0.623
  pilT Neisseria gonorrhoeae MS11

64.848

95.652

0.62
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.909

95.652

0.487
  pilU Vibrio cholerae strain A1552

41.159

100

0.412
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.406
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment