Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEL84_RS02165 Genome accession   NZ_CP168212
Coordinates   455569..456675 (+) Length   368 a.a.
NCBI ID   WP_261894725.1    Uniprot ID   -
Organism   Vibrio porteresiae strain MSSRF31     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 450569..461675
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEL84_RS02135 (ACEL84_02135) - 451230..451829 (-) 600 WP_261894717.1 XTP/dITP diphosphatase -
  ACEL84_RS02140 (ACEL84_02140) yggU 451996..452286 (-) 291 WP_261894718.1 DUF167 family protein YggU -
  ACEL84_RS02145 (ACEL84_02145) - 452286..452843 (-) 558 WP_261894720.1 YggT family protein -
  ACEL84_RS02150 (ACEL84_02150) proC 452916..453734 (-) 819 WP_261894721.1 pyrroline-5-carboxylate reductase -
  ACEL84_RS02155 (ACEL84_02155) - 453788..454495 (-) 708 WP_261894722.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEL84_RS02160 (ACEL84_02160) pilT 454521..455558 (+) 1038 WP_261894724.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEL84_RS02165 (ACEL84_02165) pilU 455569..456675 (+) 1107 WP_261894725.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEL84_RS02170 (ACEL84_02170) ruvX 456723..457145 (-) 423 WP_261894726.1 Holliday junction resolvase RuvX -
  ACEL84_RS02175 (ACEL84_02175) - 457221..457784 (-) 564 WP_261896206.1 YqgE/AlgH family protein -
  ACEL84_RS02180 (ACEL84_02180) gshB 457825..458781 (-) 957 WP_261894727.1 glutathione synthase -
  ACEL84_RS02185 (ACEL84_02185) rsmE 458795..459526 (-) 732 WP_261894728.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACEL84_RS02190 (ACEL84_02190) - 459615..460319 (-) 705 WP_261894729.1 endonuclease -
  ACEL84_RS02195 (ACEL84_02195) - 460405..460905 (-) 501 WP_261894730.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41286.83 Da        Isoelectric Point: 5.4309

>NTDB_id=1043095 ACEL84_RS02165 WP_261894725.1 455569..456675(+) (pilU) [Vibrio porteresiae strain MSSRF31]
MFLEQCLSGMITRKASDLYITVGAPVLYRVNGELQGDGDKLSFSDVNNLLNRMMDSEQVEQFYHTHEANFAIVRDSGRFR
VSAFFQREQPGAVIRRIETRIPTFDELSLPELLKDLVMSKRGLVLVVGSTGSGKSTTMAAMTGYRNQNSSGHILTVEDPI
EFVHEHQGCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSKETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVGQQLIRDKQGQGRFGAFEILLNTPRVSDLIRRGELHELKETMARSRESGMQTFDQALY
QLVVDNKITQEDAMHCADSANDLRLMFKTQGSGSSSSGSLDHVTIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1043095 ACEL84_RS02165 WP_261894725.1 455569..456675(+) (pilU) [Vibrio porteresiae strain MSSRF31]
ATGTTTTTGGAACAGTGTTTAAGTGGAATGATTACCCGTAAAGCCTCTGATTTGTATATCACAGTTGGAGCTCCGGTGTT
GTATCGTGTGAATGGTGAATTGCAAGGCGATGGTGACAAGCTGAGTTTTTCTGATGTAAACAATCTACTTAATCGCATGA
TGGACAGTGAGCAAGTCGAGCAGTTCTATCATACTCACGAAGCTAACTTTGCAATTGTGCGTGACAGTGGCCGTTTTCGC
GTCAGTGCCTTCTTTCAACGTGAACAACCTGGTGCGGTCATTCGCCGTATTGAAACGCGTATCCCAACGTTTGATGAGTT
GTCCTTACCTGAATTGCTTAAAGATTTGGTGATGAGTAAGCGTGGCTTGGTATTGGTTGTGGGATCAACGGGTTCAGGTA
AATCGACAACCATGGCGGCGATGACGGGTTATCGTAATCAAAATTCTTCTGGGCATATTTTGACGGTTGAAGATCCGATT
GAATTTGTTCATGAACATCAAGGCTGTATTGTTACTCAGCGTGAGGTGGGATTGGACACGGACAGCTATGAAGTAGCGCT
GAAAAACTCGCTGCGTCAGGCTCCTGATATGATTTTGATCGGCGAAATACGTTCGAAAGAAACTATGGAATACGCCATGA
ACTTTGCTGAAACTGGTCATTTATGTATGGCGACACTTCATGCCAATAATGCCAATCAAGCCTTAGAGCGTATTTTGCAT
TTGGTGCCTAAAGAGCAAAAAGAGCAGTTTTTGTTTGATTTATCAATGAATTTACGTGGGGTCGTTGGGCAACAATTGAT
TCGCGATAAGCAAGGTCAGGGACGTTTTGGTGCCTTTGAGATACTGCTTAATACACCGCGTGTTTCTGATTTGATTCGTC
GTGGTGAGCTACATGAATTGAAAGAAACGATGGCGCGTTCACGTGAATCTGGCATGCAAACCTTTGATCAAGCTTTGTAT
CAATTGGTAGTAGATAACAAGATTACGCAAGAAGATGCCATGCACTGCGCCGATTCAGCTAACGACTTACGATTAATGTT
CAAAACTCAAGGTAGCGGGAGCAGCAGTTCAGGCTCTCTAGATCATGTCACTATCGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

77.446

100

0.774
  pilU Pseudomonas stutzeri DSM 10701

57.104

99.457

0.568
  pilU Acinetobacter baylyi ADP1

55.84

95.38

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.425

88.859

0.386
  pilT Legionella pneumophila strain ERS1305867

40.708

92.12

0.375
  pilT Legionella pneumophila strain Lp02

40.708

92.12

0.375
  pilT Acinetobacter baylyi ADP1

40.176

92.663

0.372
  pilT Pseudomonas aeruginosa PAK

40.597

91.033

0.37
  pilT Acinetobacter baumannii D1279779

39.528

92.12

0.364
  pilT Acinetobacter baumannii strain A118

39.528

92.12

0.364
  pilT Acinetobacter nosocomialis M2

39.528

92.12

0.364

Multiple sequence alignment