Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R8721_RS03270 Genome accession   NZ_AP028321
Coordinates   678930..679964 (-) Length   344 a.a.
NCBI ID   WP_005305010.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain RYU-Ah62     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 673930..684964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8721_RS03250 (Ri1_06160) - 674748..675458 (-) 711 WP_010636176.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  R8721_RS03255 (Ri1_06170) srmB 675587..676810 (+) 1224 WP_010636177.1 ATP-dependent RNA helicase SrmB -
  R8721_RS03260 (Ri1_06180) yaaA 676987..677760 (-) 774 WP_010636178.1 peroxide stress protein YaaA -
  R8721_RS03265 (Ri1_06190) pilU 677792..678901 (-) 1110 WP_010636179.1 type IVa pilus ATPase TapU Machinery gene
  R8721_RS03270 (Ri1_06200) pilT 678930..679964 (-) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  R8721_RS03275 (Ri1_06210) - 680004..680705 (+) 702 WP_010636180.1 YggS family pyridoxal phosphate-dependent enzyme -
  R8721_RS03280 (Ri1_06220) proC 680766..681590 (+) 825 WP_010636181.1 pyrroline-5-carboxylate reductase -
  R8721_RS03285 (Ri1_06230) - 681631..682182 (+) 552 WP_005305000.1 YggT family protein -
  R8721_RS03290 (Ri1_06240) yggU 682182..682481 (+) 300 WP_005304998.1 DUF167 family protein YggU -
  R8721_RS03295 (Ri1_06250) - 682500..682919 (+) 420 WP_005304995.1 DUF4426 domain-containing protein -
  R8721_RS03300 (Ri1_06260) - 682983..683294 (-) 312 WP_005304992.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38319.12 Da        Isoelectric Point: 6.7439

>NTDB_id=104188 R8721_RS03270 WP_005305010.1 678930..679964(-) (pilT) [Aeromonas dhakensis strain RYU-Ah62]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGMVAALDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=104188 R8721_RS03270 WP_005305010.1 678930..679964(-) (pilT) [Aeromonas dhakensis strain RYU-Ah62]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAACATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGCGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAGCACCGGGAAGTACACGCCCTCATCTATGACA
TCATGAACGACCACCAGCGCAAGGAACTGGAAGAGAACTTCGAGGTGGACTTCTCGTTCGAGGTGCCAAACCTCGCTCGC
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCCGGCGCGGTATTTCGGACCATCCCCAGCACAGTGCTGAGCCTGGA
AGATCTTGATGCCCCGGAGATCTTTCGCAAGATTGCCGAATACCCCCGCGGCCTGGTGCTGGTGACCGGTCCGACCGGTT
CGGGCAAGTCCACTACCTTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAAAACAAACGCTGCCTGGTGAACCAGCGGGAAGTGCACCGCGACACCAAGAGCTTCAGCAA
CGCCTTGCGTTCGGCGCTGCGGGAAGACCCGGATATCATTCTGGTGGGTGAAATGCGCGACCTCGAGACCATTCGCCTCG
CCATGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGGATC
ATCGACGTCTTTCCCGGTGCGGAAAAAGACATGGTGCGCTCCATGCTCTCCGAATCGCTGCGGGCGGTCATCTCCCAGAC
TCTGCTCAAACGCATCGGTGGCGGCCGGGTGGCGGCCCACGAGATCATGATGGGGATCCCGGCAGTGCGTAACCTCATCC
GGGAGGACAAGATCGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGCCGCGGCATGGTGGCAGCGCTGGATGCCAAGGCCAAGGCGGTCGATCCCAACACCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Acinetobacter baumannii D1279779

76.744

100

0.767
  pilT Acinetobacter baumannii strain A118

76.744

100

0.767
  pilT Pseudomonas stutzeri DSM 10701

76.833

99.128

0.762
  pilT Pseudomonas aeruginosa PAK

75.953

99.128

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

72.647

98.837

0.718
  pilT Legionella pneumophila strain ERS1305867

72.647

98.837

0.718
  pilT Neisseria meningitidis 8013

70.796

98.547

0.698
  pilT Neisseria gonorrhoeae MS11

70.501

98.547

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407

Multiple sequence alignment