Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R8721_RS03265 Genome accession   NZ_AP028321
Coordinates   677792..678901 (-) Length   369 a.a.
NCBI ID   WP_010636179.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain RYU-Ah62     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 672792..683901
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8721_RS03245 (Ri1_06150) brnQ 673249..674556 (-) 1308 WP_005305030.1 branched-chain amino acid transport system II carrier protein -
  R8721_RS03250 (Ri1_06160) - 674748..675458 (-) 711 WP_010636176.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  R8721_RS03255 (Ri1_06170) srmB 675587..676810 (+) 1224 WP_010636177.1 ATP-dependent RNA helicase SrmB -
  R8721_RS03260 (Ri1_06180) yaaA 676987..677760 (-) 774 WP_010636178.1 peroxide stress protein YaaA -
  R8721_RS03265 (Ri1_06190) pilU 677792..678901 (-) 1110 WP_010636179.1 type IVa pilus ATPase TapU Machinery gene
  R8721_RS03270 (Ri1_06200) pilT 678930..679964 (-) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  R8721_RS03275 (Ri1_06210) - 680004..680705 (+) 702 WP_010636180.1 YggS family pyridoxal phosphate-dependent enzyme -
  R8721_RS03280 (Ri1_06220) proC 680766..681590 (+) 825 WP_010636181.1 pyrroline-5-carboxylate reductase -
  R8721_RS03285 (Ri1_06230) - 681631..682182 (+) 552 WP_005305000.1 YggT family protein -
  R8721_RS03290 (Ri1_06240) yggU 682182..682481 (+) 300 WP_005304998.1 DUF167 family protein YggU -
  R8721_RS03295 (Ri1_06250) - 682500..682919 (+) 420 WP_005304995.1 DUF4426 domain-containing protein -
  R8721_RS03300 (Ri1_06260) - 682983..683294 (-) 312 WP_005304992.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41081.22 Da        Isoelectric Point: 6.5672

>NTDB_id=104187 R8721_RS03265 WP_010636179.1 677792..678901(-) (pilU) [Aeromonas dhakensis strain RYU-Ah62]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSPEHFERYLRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSPDGRRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGSGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=104187 R8721_RS03265 WP_010636179.1 677792..678901(-) (pilU) [Aeromonas dhakensis strain RYU-Ah62]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCGCTGGCAGAAGCGCCCCTCGACAAGGCAAGGGCACTGGCGCTGGTGAAGGAGA
CCCTGAGCCCGGAGCACTTCGAGCGCTACCTGCGCACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGCATTGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCACTGCCCCCCATCTTGCAGGAGATCGCCCTGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCATCGTTCGATGT
GGCGCTGAAGAGTTCGCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACCGGTCATCTCTGCCTCGCCACCCTGCATGCCAACAACGCCAACCAGGCGCTTGATCGCATC
CTGCACCTGGTGCCCCAGGAGAAACACCGTCAGTTCCTGTTTGATCTCTCCTTCAATCTGCGCGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCCGGATGGACGGCGTCGTTTTGCCGCCTTCGAGGTGTTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGGGAGATGCACCGCCTCAAGGAGGTGATGACCAAGTCCACCGAACTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGCCGAGAACAGCTGGGTTCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.87

99.729

0.607
  pilU Pseudomonas stutzeri DSM 10701

59.829

95.122

0.569
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363

Multiple sequence alignment