Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACDI35_RS07685 Genome accession   NZ_CP167834
Coordinates   1766874..1767911 (+) Length   345 a.a.
NCBI ID   WP_003489919.1    Uniprot ID   -
Organism   Xanthomonas axonopodis pv. cajani strain NCPPB 2063     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1761874..1772911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDI35_RS07655 (ACDI35_07645) - 1762126..1762668 (-) 543 WP_372376219.1 hypothetical protein -
  ACDI35_RS07660 (ACDI35_07650) - 1762725..1763066 (-) 342 WP_372376220.1 DUF3861 family protein -
  ACDI35_RS07665 (ACDI35_07655) - 1763124..1763765 (-) 642 WP_089095696.1 M23 family metallopeptidase -
  ACDI35_RS07670 (ACDI35_07660) - 1763868..1764308 (-) 441 WP_372376677.1 HU family DNA-binding protein -
  ACDI35_RS07675 (ACDI35_07665) proC 1764872..1765726 (-) 855 WP_372376222.1 pyrroline-5-carboxylate reductase -
  ACDI35_RS07680 (ACDI35_07670) - 1765770..1766462 (-) 693 WP_057662318.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACDI35_RS07685 (ACDI35_07675) pilT 1766874..1767911 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ACDI35_RS07690 (ACDI35_07680) pilU 1768024..1769154 (+) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACDI35_RS07695 (ACDI35_07685) - 1769656..1770330 (+) 675 WP_016851096.1 YitT family protein -
  ACDI35_RS07700 (ACDI35_07690) - 1770505..1771431 (-) 927 WP_372376223.1 DUF72 domain-containing protein -
  ACDI35_RS07705 (ACDI35_07695) - 1771439..1772011 (-) 573 WP_372376224.1 DNA-3-methyladenine glycosylase I -
  ACDI35_RS07710 (ACDI35_07700) - 1772279..1772845 (+) 567 WP_372376225.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38643.30 Da        Isoelectric Point: 6.6652

>NTDB_id=1039692 ACDI35_RS07685 WP_003489919.1 1766874..1767911(+) (pilT) [Xanthomonas axonopodis pv. cajani strain NCPPB 2063]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1039692 ACDI35_RS07685 WP_003489919.1 1766874..1767911(+) (pilT) [Xanthomonas axonopodis pv. cajani strain NCPPB 2063]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGTCGCATCAATATTCCGGCTCTGGACCACAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGTCGCGATTACGAAGAATTTCTCGAGGTCGACTTCTCGTTCGAGATTCCGTCGCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACGCTGGA
AGACCTTGGCTGCCCGCCGATCTTCCGTCAGCTGATCGATCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCCGGCATGATCGACTACATCAACAAGAATGAGTACGGGCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCACGGCTTCAACGA
AGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATTCGCCTGG
CGCTGACCGCCGCCGAGACCGGCCACTTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCTGTGATTTCGCAGGC
GCTGTTGAAGAAGGTCGGCGGCGGCCGTACCGCAGCATGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTCGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCCCGCGAGTACGCCAAGGACAAGCGGATATTCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394

Multiple sequence alignment