Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACDI35_RS07690 Genome accession   NZ_CP167834
Coordinates   1768024..1769154 (+) Length   376 a.a.
NCBI ID   WP_003489921.1    Uniprot ID   A0AAI7ZGP8
Organism   Xanthomonas axonopodis pv. cajani strain NCPPB 2063     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1763024..1774154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDI35_RS07665 (ACDI35_07655) - 1763124..1763765 (-) 642 WP_089095696.1 M23 family metallopeptidase -
  ACDI35_RS07670 (ACDI35_07660) - 1763868..1764308 (-) 441 WP_372376677.1 HU family DNA-binding protein -
  ACDI35_RS07675 (ACDI35_07665) proC 1764872..1765726 (-) 855 WP_372376222.1 pyrroline-5-carboxylate reductase -
  ACDI35_RS07680 (ACDI35_07670) - 1765770..1766462 (-) 693 WP_057662318.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACDI35_RS07685 (ACDI35_07675) pilT 1766874..1767911 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ACDI35_RS07690 (ACDI35_07680) pilU 1768024..1769154 (+) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACDI35_RS07695 (ACDI35_07685) - 1769656..1770330 (+) 675 WP_016851096.1 YitT family protein -
  ACDI35_RS07700 (ACDI35_07690) - 1770505..1771431 (-) 927 WP_372376223.1 DUF72 domain-containing protein -
  ACDI35_RS07705 (ACDI35_07695) - 1771439..1772011 (-) 573 WP_372376224.1 DNA-3-methyladenine glycosylase I -
  ACDI35_RS07710 (ACDI35_07700) - 1772279..1772845 (+) 567 WP_372376225.1 YqgE/AlgH family protein -
  ACDI35_RS07715 (ACDI35_07705) ruvX 1772838..1773305 (+) 468 WP_003489932.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42044.26 Da        Isoelectric Point: 6.8286

>NTDB_id=1039693 ACDI35_RS07690 WP_003489921.1 1768024..1769154(+) (pilU) [Xanthomonas axonopodis pv. cajani strain NCPPB 2063]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1039693 ACDI35_RS07690 WP_003489921.1 1768024..1769154(+) (pilU) [Xanthomonas axonopodis pv. cajani strain NCPPB 2063]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCGATTACCCAGACCCCGCTGACCGCGCAGCAAAGTCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGTTTTCGTGTGAGCTGCTTCTACCAGCGTAATCAGGTGGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCTTGCCGCCGGTGATCAAGACGCTGTCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGGACTGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGTAACCAGAACTCGACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGTTGTGTACGCTGCACGCCAACAACGCCAACCAGGCGATG
GATCGCATCATCAACTTCTTCCCCGAAGATCGCCGCAACCAGCTATTGATGGATCTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGATGGCCGCAGCCGTCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTATATCCGTGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCTTGTTCGAGCTCTACCAGGCCGGCGAAATCAGCTACGAAGATGCGTTGCGCTACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCTCGCAGGGGGGCGATGCCAAGACCTTGTCGCAGGGCCTGGACGGTGTAGAAATTTCCG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.457

94.947

0.394
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364

Multiple sequence alignment