Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABRP17_RS04320 Genome accession   NZ_CP167826
Coordinates   998352..999389 (-) Length   345 a.a.
NCBI ID   WP_188324779.1    Uniprot ID   A0A7H1AJJ7
Organism   Stenotrophomonas sp. WHRI 8082     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 993352..1004389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABRP17_RS04300 (ABRP17_004300) - 994178..994642 (-) 465 WP_188324775.1 cupin domain-containing protein -
  ABRP17_RS04305 (ABRP17_004305) - 994781..995674 (+) 894 WP_349984905.1 LysR family transcriptional regulator -
  ABRP17_RS04310 (ABRP17_004310) - 995761..996744 (-) 984 WP_349984906.1 aldo/keto reductase -
  ABRP17_RS04315 (ABRP17_004315) pilU 997116..998210 (-) 1095 WP_197974053.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABRP17_RS04320 (ABRP17_004320) pilT 998352..999389 (-) 1038 WP_188324779.1 type IV pilus twitching motility protein PilT Machinery gene
  ABRP17_RS04325 (ABRP17_004325) - 999477..1000154 (+) 678 WP_188324780.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABRP17_RS04330 (ABRP17_004330) proC 1000173..1000994 (+) 822 WP_349984907.1 pyrroline-5-carboxylate reductase -
  ABRP17_RS04335 (ABRP17_004335) - 1001156..1002052 (-) 897 WP_349984908.1 LysR family transcriptional regulator -
  ABRP17_RS04340 (ABRP17_004340) - 1002148..1002750 (+) 603 WP_188324783.1 short chain dehydrogenase -
  ABRP17_RS04345 (ABRP17_004345) soxR 1002946..1003380 (-) 435 WP_349984909.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38616.37 Da        Isoelectric Point: 6.6653

>NTDB_id=1039634 ABRP17_RS04320 WP_188324779.1 998352..999389(-) (pilT) [Stenotrophomonas sp. WHRI 8082]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1039634 ABRP17_RS04320 WP_188324779.1 998352..999389(-) (pilT) [Stenotrophomonas sp. WHRI 8082]
ATGGATATCGCCGAGCTGTTGGCGTTTTCCGTAAAGAACAAAGCATCCGACCTGCACCTGTCCGCAGGCCTGCCGCCGAT
GATCCGTGTCGACGGCGATGTCCGTCGCATCAACATTCCTGCGCTGGACCACAAGCAGGTGCATGCGCTTGTCTACGACA
TCATGTCGGACAAGCAGCGTCGCGATTACGAGGAGTTCCTCGAGGTCGATTTCTCGTTCGAGATTCCGTCGCTCGCGCGC
TTCCGCGTCAATGCCTTCAACCAGAACCGTGGCGCGGGTGCGGTGTTCCGTACCATTCCGTCCGAGGTGCTGACCTTGGA
GGACCTGGCCTGCCCGCCGCTGTTCCGCGAGGTCATCCAGCAGCCGCAGGGGCTGATCCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACGACGCTTGCCGCGATGATCGACTACATCAACAAGAACGAGTACGGACACATCCTCACCGTCGAGGAT
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGATACGCATGGCTTCAACGA
AGCGCTGCGTTCGGCGCTGCGCGAAGATCCGGACATCATCCTGGTCGGCGAGCTCCGCGATCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCATCTTGTGTTCGGTACGCTGCACACCAGTTCGGCCGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCCGCCGGCGAAAAACCGATGGTGCGCTCCATGCTGTCGGAGTCGCTGCGTGCGGTCATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGTGGACGCACGGCGGCCTGGGAAATCATGGTCGGCACGCCCGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGTCAGCAGTACGGCATGATGACCCTGGACCAGCATCTG
CAGGATCTGGTCAAGCGCAGCCTGATCACGCGCAACCAAGCCAAAGAGTACGCCAAGGACAAGCGCCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H1AJJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter nosocomialis M2

79.07

99.71

0.788
  pilT Acinetobacter baumannii D1279779

79.07

99.71

0.788
  pilT Acinetobacter baumannii strain A118

79.07

99.71

0.788
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.517

95.362

0.672
  pilT Vibrio cholerae strain A1552

70.517

95.362

0.672
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.118

98.261

0.394
  pilU Acinetobacter baylyi ADP1

39.412

98.551

0.388

Multiple sequence alignment