Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABRP17_RS04315 Genome accession   NZ_CP167826
Coordinates   997116..998210 (-) Length   364 a.a.
NCBI ID   WP_197974053.1    Uniprot ID   -
Organism   Stenotrophomonas sp. WHRI 8082     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 992116..1003210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABRP17_RS04290 (ABRP17_004290) - 992158..992646 (-) 489 WP_349984903.1 BBE domain-containing protein -
  ABRP17_RS04295 (ABRP17_004295) - 992653..993786 (-) 1134 WP_349984904.1 FAD-binding protein -
  ABRP17_RS04300 (ABRP17_004300) - 994178..994642 (-) 465 WP_188324775.1 cupin domain-containing protein -
  ABRP17_RS04305 (ABRP17_004305) - 994781..995674 (+) 894 WP_349984905.1 LysR family transcriptional regulator -
  ABRP17_RS04310 (ABRP17_004310) - 995761..996744 (-) 984 WP_349984906.1 aldo/keto reductase -
  ABRP17_RS04315 (ABRP17_004315) pilU 997116..998210 (-) 1095 WP_197974053.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABRP17_RS04320 (ABRP17_004320) pilT 998352..999389 (-) 1038 WP_188324779.1 type IV pilus twitching motility protein PilT Machinery gene
  ABRP17_RS04325 (ABRP17_004325) - 999477..1000154 (+) 678 WP_188324780.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABRP17_RS04330 (ABRP17_004330) proC 1000173..1000994 (+) 822 WP_349984907.1 pyrroline-5-carboxylate reductase -
  ABRP17_RS04335 (ABRP17_004335) - 1001156..1002052 (-) 897 WP_349984908.1 LysR family transcriptional regulator -
  ABRP17_RS04340 (ABRP17_004340) - 1002148..1002750 (+) 603 WP_188324783.1 short chain dehydrogenase -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40620.66 Da        Isoelectric Point: 6.8285

>NTDB_id=1039633 ABRP17_RS04315 WP_197974053.1 997116..998210(-) (pilU) [Stenotrophomonas sp. WHRI 8082]
MAHQRASDLFITAGMPPAIKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVDELALPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNHNSTGHIITIEDPIEFVHRHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRIINFFPEDRR
NQLLMDLSLNIKGVVAQQLIPSPDGRSRKVAMEIMLGTPLVQDYIREGEIHKLKEVMKESTQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=1039633 ABRP17_RS04315 WP_197974053.1 997116..998210(-) (pilU) [Stenotrophomonas sp. WHRI 8082]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACGGCCGGCATGCCGCCGGCGATCAAGGTCAACGGCAAGATCTCGCC
GATCACCCAGACCCCGCTGACGCCGCAGCAGAGCCGCGATCTGGTGTTGAACGTGATGACGCCGGCGCAGCGCGAAGAGT
TCGAGAAGACGCACGAATGTAATTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGTGTCAGTTGCTTCTACCAGCGC
AACCAGGTCGGCATGGTGCTGCGCCGGATTGAAACGCGCATCCCCACCGTGGATGAGCTGGCGCTGCCGCCGATCATCAA
GACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGTGCGACCGGTACCGGCAAGTCGACCTCGTTGGCGGCGA
TGATCGGCTACCGCAACCACAACTCGACCGGCCATATCATCACCATCGAGGATCCGATCGAGTTCGTGCACCGCCACGAA
GGCTGCATCATCACCCAGCGCGAGGTCGGCATCGATACGGACAGCTGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGC
ACCGGACGTGATCATGATCGGCGAGGTCCGTACCCGCGAAGGCATGGACCATGCGATTGCCTTCGCTGAGACCGGTCATC
TGGTGCTGTGCACGCTGCATGCGAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCCGAAGATCGCCGC
AACCAGCTGCTGATGGATCTGTCGCTGAACATCAAGGGCGTGGTCGCGCAGCAGCTGATTCCCTCGCCGGACGGACGCTC
GCGCAAGGTCGCCATGGAGATCATGCTCGGTACGCCGCTGGTGCAGGATTACATCCGCGAAGGCGAGATCCACAAGCTCA
AGGAAGTCATGAAGGAGTCCACCCAGCTGGGCATGAAGACCTTCGACCAGAGCTTGTTCGAGCTGTACCAGGCGGGCGAG
ATCAGCTACGAAGACGCGCTGCGGTATGCCGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTGAGCCAGGGCGGCGA
TGCCCGCACACTGTCGCAGGGCCTGGACGGTGTGGAGATTTCCGAAGTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(105-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.877

98.352

0.668
  pilU Acinetobacter baylyi ADP1

64.08

95.604

0.613
  pilU Vibrio cholerae strain A1552

53.314

95.33

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.319

94.78

0.401
  pilT Acinetobacter baumannii strain A118

40.909

90.659

0.371
  pilT Acinetobacter nosocomialis M2

40.909

90.659

0.371
  pilT Acinetobacter baumannii D1279779

40.909

90.659

0.371
  pilT Pseudomonas aeruginosa PAK

40.923

89.286

0.365
  pilT Legionella pneumophila strain Lp02

41.176

88.736

0.365
  pilT Legionella pneumophila strain ERS1305867

41.176

88.736

0.365

Multiple sequence alignment