Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilT   Type   Machinery gene
Locus tag   ACDJ02_RS13430 Genome accession   NZ_CP167777
Coordinates   2846209..2847246 (+) Length   345 a.a.
NCBI ID   WP_004990994.1    Uniprot ID   A0A2N6VBT8
Organism   Acinetobacter ursingii strain E5131942     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2784310..2864331 2846209..2847246 within 0


Gene organization within MGE regions


Location: 2784310..2864331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDJ02_RS13185 (ACDJ02_13185) - 2784354..2785553 (-) 1200 WP_004890115.1 integrase arm-type DNA-binding domain-containing protein -
  ACDJ02_RS13190 (ACDJ02_13190) - 2786174..2786614 (-) 441 WP_004990861.1 PaaI family thioesterase -
  ACDJ02_RS13195 (ACDJ02_13195) - 2786826..2788895 (-) 2070 WP_104794683.1 bifunctional diguanylate cyclase/phosphodiesterase -
  ACDJ02_RS13200 (ACDJ02_13200) ilvC 2789328..2790344 (-) 1017 WP_004990871.1 ketol-acid reductoisomerase -
  ACDJ02_RS13205 (ACDJ02_13205) ilvN 2790464..2790955 (-) 492 WP_004990873.1 acetolactate synthase small subunit -
  ACDJ02_RS13210 (ACDJ02_13210) - 2790955..2792679 (-) 1725 WP_005002924.1 acetolactate synthase 3 large subunit -
  ACDJ02_RS13215 (ACDJ02_13215) - 2793212..2793583 (+) 372 WP_004996056.1 DUF4124 domain-containing protein -
  ACDJ02_RS13220 (ACDJ02_13220) leuS 2793749..2796385 (+) 2637 WP_151724719.1 leucine--tRNA ligase -
  ACDJ02_RS13225 (ACDJ02_13225) lptE 2796599..2797126 (+) 528 WP_151724720.1 LPS assembly lipoprotein LptE -
  ACDJ02_RS13230 (ACDJ02_13230) holA 2797150..2798139 (+) 990 WP_151724721.1 DNA polymerase III subunit delta -
  ACDJ02_RS13235 (ACDJ02_13235) - 2798242..2799594 (+) 1353 WP_151724722.1 MacA family efflux pump subunit -
  ACDJ02_RS13240 (ACDJ02_13240) - 2799597..2801588 (+) 1992 WP_004996045.1 MacB family efflux pump subunit -
  ACDJ02_RS13245 (ACDJ02_13245) - 2801600..2803003 (+) 1404 WP_151724723.1 efflux transporter outer membrane subunit -
  ACDJ02_RS13250 (ACDJ02_13250) - 2803360..2805630 (-) 2271 WP_151724724.1 TonB-dependent receptor -
  ACDJ02_RS13255 (ACDJ02_13255) - 2805639..2806886 (-) 1248 WP_371863724.1 MFS transporter -
  ACDJ02_RS13260 (ACDJ02_13260) - 2806865..2807254 (-) 390 WP_049174748.1 hypothetical protein -
  ACDJ02_RS13265 (ACDJ02_13265) - 2807251..2807886 (-) 636 WP_151724726.1 GNAT family N-acetyltransferase -
  ACDJ02_RS13270 (ACDJ02_13270) - 2807900..2809723 (-) 1824 WP_151724727.1 IucA/IucC family siderophore biosynthesis protein -
  ACDJ02_RS13275 (ACDJ02_13275) - 2809720..2811198 (-) 1479 WP_151724728.1 lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family protein -
  ACDJ02_RS13280 (ACDJ02_13280) - 2811191..2812975 (-) 1785 WP_151724729.1 IucA/IucC family siderophore biosynthesis protein -
  ACDJ02_RS13285 (ACDJ02_13285) - 2813591..2814496 (-) 906 WP_151725229.1 LysR family transcriptional regulator -
  ACDJ02_RS13290 (ACDJ02_13290) - 2814753..2815415 (+) 663 WP_151725228.1 RraA family protein -
  ACDJ02_RS13295 (ACDJ02_13295) - 2815415..2816626 (+) 1212 WP_151725227.1 pyridoxal phosphate-dependent aminotransferase -
  ACDJ02_RS13300 (ACDJ02_13300) - 2816765..2818078 (+) 1314 WP_228268498.1 cation:dicarboxylate symporter family transporter -
  ACDJ02_RS13305 (ACDJ02_13305) - 2818089..2819360 (+) 1272 WP_151725226.1 Glu/Leu/Phe/Val dehydrogenase -
  ACDJ02_RS13310 (ACDJ02_13310) - 2819922..2820188 (+) 267 WP_004990919.1 hypothetical protein -
  ACDJ02_RS13315 (ACDJ02_13315) - 2820578..2821786 (+) 1209 WP_262458484.1 MFS transporter -
  ACDJ02_RS13320 (ACDJ02_13320) - 2821888..2823336 (-) 1449 WP_004990925.1 trehalose-6-phosphate synthase -
  ACDJ02_RS13325 (ACDJ02_13325) otsB 2823329..2824174 (-) 846 WP_280095304.1 trehalose-phosphatase -
  ACDJ02_RS13330 (ACDJ02_13330) - 2824609..2825082 (+) 474 WP_004990929.1 pyridoxamine 5'-phosphate oxidase family protein -
  ACDJ02_RS13335 (ACDJ02_13335) - 2825693..2825953 (+) 261 WP_004990931.1 hypothetical protein -
  ACDJ02_RS13340 (ACDJ02_13340) - 2826429..2826895 (+) 467 Protein_2599 BLUF domain-containing protein -
  ACDJ02_RS13345 (ACDJ02_13345) - 2827144..2827248 (-) 105 WP_372376516.1 hypothetical protein -
  ACDJ02_RS13350 (ACDJ02_13350) - 2827342..2827497 (-) 156 WP_005002873.1 hypothetical protein -
  ACDJ02_RS13355 (ACDJ02_13355) - 2827565..2828350 (-) 786 WP_372375240.1 hypothetical protein -
  ACDJ02_RS13360 (ACDJ02_13360) - 2828442..2829370 (-) 929 Protein_2603 IS5-like element ISAba10 family transposase -
  ACDJ02_RS13365 (ACDJ02_13365) - 2829622..2831037 (+) 1416 WP_372375241.1 PLP-dependent aminotransferase family protein -
  ACDJ02_RS13370 (ACDJ02_13370) - 2831119..2832663 (-) 1545 WP_004990955.1 DHA2 family efflux MFS transporter permease subunit -
  ACDJ02_RS13375 (ACDJ02_13375) - 2832667..2833821 (-) 1155 WP_004990956.1 HlyD family efflux transporter periplasmic adaptor subunit -
  ACDJ02_RS13380 (ACDJ02_13380) - 2834094..2834591 (-) 498 WP_004990957.1 thioesterase family protein -
  ACDJ02_RS13385 (ACDJ02_13385) hemB 2834700..2835713 (+) 1014 WP_004990964.1 porphobilinogen synthase -
  ACDJ02_RS13390 (ACDJ02_13390) thiO 2835773..2836894 (+) 1122 WP_004990968.1 glycine oxidase ThiO -
  ACDJ02_RS13395 (ACDJ02_13395) - 2836920..2837372 (-) 453 WP_280095306.1 ABZJ_00895 family protein -
  ACDJ02_RS13400 (ACDJ02_13400) - 2837730..2838305 (+) 576 WP_262458489.1 hypothetical protein -
  ACDJ02_RS13405 (ACDJ02_13405) - 2838455..2839720 (+) 1266 WP_372376517.1 exonuclease SbcCD subunit D -
  ACDJ02_RS13410 (ACDJ02_13410) - 2839730..2843326 (+) 3597 WP_262545105.1 AAA family ATPase -
  ACDJ02_RS13415 (ACDJ02_13415) - 2843510..2844301 (+) 792 WP_262545103.1 transglutaminase family protein -
  ACDJ02_RS13420 (ACDJ02_13420) - 2844403..2845248 (-) 846 WP_044436293.1 undecaprenyl-diphosphate phosphatase -
  ACDJ02_RS13425 (ACDJ02_13425) - 2845399..2846085 (-) 687 WP_270907916.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACDJ02_RS13430 (ACDJ02_13430) pilT 2846209..2847246 (+) 1038 WP_004990994.1 type IV pilus twitching motility protein PilT Machinery gene
  ACDJ02_RS13435 (ACDJ02_13435) pilU 2847274..2848392 (+) 1119 WP_004990996.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACDJ02_RS13440 (ACDJ02_13440) fur 2848466..2848906 (-) 441 WP_004990998.1 ferric iron uptake transcriptional regulator -
  ACDJ02_RS13445 (ACDJ02_13445) - 2849018..2849416 (+) 399 WP_004991001.1 outer membrane protein assembly factor BamE -
  ACDJ02_RS13450 (ACDJ02_13450) - 2849460..2849780 (-) 321 WP_004991003.1 RnfH family protein -
  ACDJ02_RS13455 (ACDJ02_13455) - 2849777..2850862 (-) 1086 WP_336148870.1 hypothetical protein -
  ACDJ02_RS13460 (ACDJ02_13460) - 2851025..2851474 (-) 450 WP_004991008.1 bacteriohemerythrin -
  ACDJ02_RS13465 (ACDJ02_13465) - 2851810..2852658 (-) 849 WP_035254157.1 class II glutamine amidotransferase -
  ACDJ02_RS13470 (ACDJ02_13470) - 2852762..2853541 (-) 780 WP_151725111.1 GNAT family N-acetyltransferase -
  ACDJ02_RS13475 (ACDJ02_13475) argB 2853662..2854573 (-) 912 WP_004991011.1 acetylglutamate kinase -
  ACDJ02_RS13480 (ACDJ02_13480) - 2854584..2856008 (-) 1425 WP_151725112.1 phosphomannomutase/phosphoglucomutase -
  ACDJ02_RS13485 (ACDJ02_13485) dut 2856187..2856639 (-) 453 WP_262465994.1 dUTP diphosphatase -
  ACDJ02_RS13490 (ACDJ02_13490) - 2856664..2858703 (-) 2040 WP_120370058.1 UvrD-helicase domain-containing protein -
  ACDJ02_RS13495 (ACDJ02_13495) - 2858825..2859481 (-) 657 WP_336165364.1 OmpA family protein -
  ACDJ02_RS13500 (ACDJ02_13500) - 2859731..2860639 (+) 909 Protein_2631 acyltransferase -
  ACDJ02_RS13505 (ACDJ02_13505) - 2860689..2861741 (+) 1053 WP_004995954.1 hypothetical protein -
  ACDJ02_RS13510 (ACDJ02_13510) minC 2861869..2862591 (+) 723 WP_004991019.1 septum site-determining protein MinC -
  ACDJ02_RS13515 (ACDJ02_13515) minD 2862658..2863470 (+) 813 WP_004991021.1 septum site-determining protein MinD -
  ACDJ02_RS13520 (ACDJ02_13520) minE 2863473..2863745 (+) 273 WP_005002814.1 cell division topological specificity factor MinE -
  ACDJ02_RS13525 (ACDJ02_13525) - 2863890..2864177 (+) 288 WP_004995952.1 PA4642 family protein -

Sequence


Protein


Download         Length: 345 a.a.        Molecular weight: 38313.90 Da        Isoelectric Point: 6.5052

>NTDB_id=1039324 ACDJ02_RS13430 WP_004990994.1 2846209..2847246(+) (pilT) [Acinetobacter ursingii strain E5131942]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHRLVYDIMNDKQRRDFEEQLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMDDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGVISPQTARTAAKSPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1039324 ACDJ02_RS13430 WP_004990994.1 2846209..2847246(+) (pilT) [Acinetobacter ursingii strain E5131942]
ATGGATATCACAGAATTACTCGCCTTTTCGGTTAAAAACGGTGCATCAGATTTACATCTTTCCGCAGGAATGCCACCGAT
GATTCGTGTCGATGGTGAGGTTCGTCGTATTAATTTACCTGCACTGGAACACAAAGAAGTTCATCGTCTTGTTTATGACA
TTATGAATGATAAACAACGTCGTGACTTTGAAGAACAACTTGAAACTGACTTTTCCTTTGAAGTTCCAAACATTGCGCGT
TTTCGTGTCAATGCCTTTAACCAAAATCGTGGTGCAGGTGCTGTGTTCCGTACCATTCCGTCTAAAGTTCTCACCATGGA
TGACTTGGGTTTAGGACAAATCTTTAAGGATATTTGTGATTATCCACGTGGTATTGTACTGGTTACAGGCCCAACGGGTT
CGGGTAAATCAACCACGCTTGCCGCCATGCTAGATTATATCAATGACAATCGCTATGACCATATTTTAACCGTAGAAGAT
CCGATCGAATTTGTACACCAATCCAAAAAGTGCCTGATTAACCAACGTGAAGTACATCGTGATACTCATGGCTTTAATGA
AGCACTACGTTCAGCACTACGTGAAGACCCTGATATTATTCTTGTCGGTGAGATGCGCGACCTTGAAACAATTCGTTTGG
CTTTGACCGCCGCAGAAACGGGTCACTTGGTATTTGGTACATTGCATACCACATCTGCTGCAAAAACCATTGACCGTGTA
ATTGACGTATTCCCTGCTGAAGAAAAAGATATGGTTCGCGCCATGCTATCTGAATCTTTACAAGCCGTTATTTCTCAAAC
ATTATTGAAGAAAAATGGCGGTGGCCGTGTCGCAGCGCATGAGATTATGATCGGGATACCTGCGATCCGTAACTTAATCC
GTGAAAATAAAGTCGCGCAAATGTACTCTGCTATTCAGACAGGTGCCAATTATGGCATGACCACACTAGATCAAAGCCTC
AAAACATTGGTTTCAAAAGGCGTGATCAGTCCACAAACTGCGCGAACAGCTGCCAAATCGCCAGAATCCTTCCTATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A2N6VBT8

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

97.101

100

0.971

  pilT Acinetobacter baumannii D1279779

96.812

100

0.968

  pilT Acinetobacter baumannii strain A118

96.812

100

0.968

  pilT Acinetobacter baylyi ADP1

88.372

99.71

0.881

  pilT Pseudomonas aeruginosa PAK

83.721

99.71

0.835

  pilT Pseudomonas stutzeri DSM 10701

83.14

99.71

0.829

  pilT Legionella pneumophila strain Lp02

75

99.71

0.748

  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748

  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.345

97.391

0.675

  pilT Vibrio cholerae strain A1552

69.345

97.391

0.675

  pilT Neisseria meningitidis 8013

67.246

100

0.672

  pilT Neisseria gonorrhoeae MS11

66.957

100

0.67

  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513

  pilU Vibrio cholerae strain A1552

41.349

98.841

0.409

  pilU Pseudomonas stutzeri DSM 10701

40.762

98.841

0.403

  pilU Acinetobacter baylyi ADP1

39.595

100

0.397


Multiple sequence alignment