Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QUF32_RS02020 Genome accession   NZ_AP028128
Coordinates   422577..423614 (+) Length   345 a.a.
NCBI ID   WP_024374563.1    Uniprot ID   -
Organism   Vibrio fluvialis strain IDH05335     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417577..428614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUF32_RS01985 (KKIDH5335_03460) hemW 417804..418976 (-) 1173 WP_100268565.1 radical SAM family heme chaperone HemW -
  QUF32_RS01990 (KKIDH5335_03470) - 418976..419575 (-) 600 WP_024374557.1 XTP/dITP diphosphatase -
  QUF32_RS01995 (KKIDH5335_03480) - 419599..420030 (-) 432 WP_024374558.1 DUF4426 domain-containing protein -
  QUF32_RS02000 (KKIDH5335_03490) yggU 420090..420380 (-) 291 WP_024374559.1 DUF167 family protein YggU -
  QUF32_RS02005 (KKIDH5335_03500) - 420380..420937 (-) 558 WP_024374560.1 YggT family protein -
  QUF32_RS02010 (KKIDH5335_03510) proC 420989..421807 (-) 819 WP_222741226.1 pyrroline-5-carboxylate reductase -
  QUF32_RS02015 (KKIDH5335_03520) - 421844..422551 (-) 708 WP_172567522.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUF32_RS02020 (KKIDH5335_03530) pilT 422577..423614 (+) 1038 WP_024374563.1 type IV pilus twitching motility protein PilT Machinery gene
  QUF32_RS02025 (KKIDH5335_03540) pilU 423626..424732 (+) 1107 WP_024374564.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUF32_RS02030 (KKIDH5335_03550) ruvX 424794..425219 (-) 426 WP_222738944.1 Holliday junction resolvase RuvX -
  QUF32_RS02035 (KKIDH5335_03560) - 425281..425844 (-) 564 WP_032080468.1 YqgE/AlgH family protein -
  QUF32_RS02040 (KKIDH5335_03570) gshB 425884..426840 (-) 957 WP_024374566.1 glutathione synthase -
  QUF32_RS02045 (KKIDH5335_03580) rsmE 426853..427584 (-) 732 WP_032080467.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QUF32_RS02050 (KKIDH5335_03590) - 427681..428382 (-) 702 WP_061056909.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38227.83 Da        Isoelectric Point: 6.5038

>NTDB_id=103929 QUF32_RS02020 WP_024374563.1 422577..423614(+) (pilT) [Vibrio fluvialis strain IDH05335]
MDISELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHADVHRLIFEIMNDAQRSEYEEKLEVDFSFALPDIGR
FRVNAFHQSRGASAVFRTIPTVIPTLDQLEAPEIFHKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINATHNKHVLTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRLGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGASHGMQTMEQHA
KQLIAQGLVDPAEVQKKIELEMSAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=103929 QUF32_RS02020 WP_024374563.1 422577..423614(+) (pilT) [Vibrio fluvialis strain IDH05335]
ATGGATATCTCTGAGTTACTGGAATTTAGTGTAAAGCATAATGCGTCAGATCTACATCTTTCTGCGGGTGTTCCTCCTAT
GGTACGCATCGATGGTGAAGTGAGAAAGCTTGGCGTACCTGCGTTTACACACGCTGATGTGCATCGATTAATTTTTGAAA
TTATGAATGATGCCCAGCGCAGTGAGTACGAAGAAAAGCTGGAAGTGGACTTTTCCTTTGCGTTACCGGACATCGGCCGA
TTTCGTGTCAACGCTTTCCATCAGTCGCGCGGCGCTTCTGCGGTATTTCGTACCATTCCGACCGTGATCCCGACTCTGGA
CCAGCTTGAGGCTCCGGAAATTTTCCACAAAATCGCCAATTACGAAAAAGGTTTGGTGCTGGTGACCGGACCGACTGGCT
CGGGTAAATCAACCACGTTAGCGGCGATGGTCGATTACATCAACGCAACCCATAACAAGCATGTGCTGACTATCGAAGAT
CCGATTGAGTTTGTGCACAGTAACAACAAATGCTTGATCAACCAACGTGAAGTACACCGCGATACCCACAGTTTTAAAAA
CGCGCTGCGCAGCGCATTGCGTGAAGATCCGGATGTGATTCTGGTCGGTGAGTTGCGCGATCAGGAAACCATCAGCTTAG
CGCTGACGGCGGCGGAAACCGGCCACTTGGTGTTCGGCACGCTGCACACCAGTTCGGCGGCGAAAACGATTGACCGCATC
ATCGATGTGTTCCCGGGCAGCGACAAAGACATGGTACGTTCAATGTTGTCAGAATCGCTGCGTGCGGTGATTGCGCAAAA
ACTGCTCAAACGTCTGGGTGGCGGGCGAGTGGCGTGTCATGAAATCATGTTGGCGACGCCTGCTATCCGTAACCTGATTC
GCGAAGATAAAGTCGCGCAGATGTATTCGATCATTCAGACTGGCGCCTCCCATGGAATGCAGACTATGGAGCAGCATGCC
AAACAGTTGATCGCGCAAGGGCTGGTGGATCCGGCAGAAGTGCAGAAGAAAATTGAACTGGAAATGTCCGCGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

92.754

100

0.928
  pilT Vibrio cholerae strain A1552

92.754

100

0.928
  pilT Acinetobacter baumannii D1279779

71.601

95.942

0.687
  pilT Acinetobacter baumannii strain A118

71.601

95.942

0.687
  pilT Acinetobacter nosocomialis M2

71.299

95.942

0.684
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

67.059

98.551

0.661
  pilT Legionella pneumophila strain Lp02

67.173

95.362

0.641
  pilT Legionella pneumophila strain ERS1305867

67.173

95.362

0.641
  pilT Neisseria meningitidis 8013

62.059

98.551

0.612
  pilT Neisseria gonorrhoeae MS11

61.765

98.551

0.609
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.424

95.652

0.501
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.867

96.232

0.403
  pilU Acinetobacter baylyi ADP1

40.312

92.754

0.374

Multiple sequence alignment