Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QUF32_RS02025 Genome accession   NZ_AP028128
Coordinates   423626..424732 (+) Length   368 a.a.
NCBI ID   WP_024374564.1    Uniprot ID   -
Organism   Vibrio fluvialis strain IDH05335     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 418626..429732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUF32_RS01990 (KKIDH5335_03470) - 418976..419575 (-) 600 WP_024374557.1 XTP/dITP diphosphatase -
  QUF32_RS01995 (KKIDH5335_03480) - 419599..420030 (-) 432 WP_024374558.1 DUF4426 domain-containing protein -
  QUF32_RS02000 (KKIDH5335_03490) yggU 420090..420380 (-) 291 WP_024374559.1 DUF167 family protein YggU -
  QUF32_RS02005 (KKIDH5335_03500) - 420380..420937 (-) 558 WP_024374560.1 YggT family protein -
  QUF32_RS02010 (KKIDH5335_03510) proC 420989..421807 (-) 819 WP_222741226.1 pyrroline-5-carboxylate reductase -
  QUF32_RS02015 (KKIDH5335_03520) - 421844..422551 (-) 708 WP_172567522.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUF32_RS02020 (KKIDH5335_03530) pilT 422577..423614 (+) 1038 WP_024374563.1 type IV pilus twitching motility protein PilT Machinery gene
  QUF32_RS02025 (KKIDH5335_03540) pilU 423626..424732 (+) 1107 WP_024374564.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUF32_RS02030 (KKIDH5335_03550) ruvX 424794..425219 (-) 426 WP_222738944.1 Holliday junction resolvase RuvX -
  QUF32_RS02035 (KKIDH5335_03560) - 425281..425844 (-) 564 WP_032080468.1 YqgE/AlgH family protein -
  QUF32_RS02040 (KKIDH5335_03570) gshB 425884..426840 (-) 957 WP_024374566.1 glutathione synthase -
  QUF32_RS02045 (KKIDH5335_03580) rsmE 426853..427584 (-) 732 WP_032080467.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QUF32_RS02050 (KKIDH5335_03590) - 427681..428382 (-) 702 WP_061056909.1 endonuclease -
  QUF32_RS02055 (KKIDH5335_03600) - 428501..429001 (-) 501 WP_222741227.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41203.14 Da        Isoelectric Point: 6.1909

>NTDB_id=103930 QUF32_RS02025 WP_024374564.1 423626..424732(+) (pilU) [Vibrio fluvialis strain IDH05335]
MQLEECLQGMMTHKASDLYITVGAPVLYRVDGELRAQGEKLDEAQVSALLAAMMDEARHADYLTTREANFAIVRDLGRFR
VSAFFQRELPGAVIRRIETQIPTFDELKLPAVLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRSGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEVLLNSPRVSDIIRRGDLHELKSTMAKSKEVGMQTFDQALY
QLVVENKISEQDALHCADSANDLRLMLKTQRGDVSSSGSLDHVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=103930 QUF32_RS02025 WP_024374564.1 423626..424732(+) (pilU) [Vibrio fluvialis strain IDH05335]
ATGCAACTCGAGGAGTGCCTGCAAGGCATGATGACGCATAAAGCGTCAGATCTATATATTACTGTGGGCGCCCCCGTGCT
GTACCGGGTCGATGGTGAACTGCGCGCGCAGGGTGAGAAGCTGGATGAAGCGCAGGTCAGCGCGCTGCTGGCTGCGATGA
TGGATGAGGCGCGCCATGCGGACTATCTGACGACGCGTGAAGCTAACTTTGCCATCGTGCGTGATTTGGGGCGTTTTCGC
GTCAGCGCCTTTTTTCAGCGCGAACTACCCGGTGCGGTGATTCGCCGCATAGAAACGCAGATTCCGACCTTTGATGAACT
CAAACTGCCAGCGGTGCTGAAAGATCTCTCGATTGCCAAACGCGGTTTAGTGTTGGTGGTTGGGGCGACAGGATCGGGGA
AATCGACTACCATGGCCGCGATGACTGGCTATCGCAACACGCACCGCAGCGGGCATATTCTCACCGTCGAAGATCCGATT
GAATTTGTCCATGAGCACCAGCGCTGCATCGTGACACAGCGTGAAGTGGGATTGGACACCGAAAGCTATGAAGTGGCGCT
GAAGAACTCGCTACGCCAGGCGCCGGATATGATTTTGATCGGTGAGATCCGCAGCCGTGAAACCATGGAATACGCAATGA
CCTTCGCCGAAACCGGTCACTTATGCATGGCAACTCTGCACGCCAACAACGCCAACCAAGCGCTCGAACGTATTTTGCAT
CTGGTGCCGAAAGAGCAGCGTGAACAATTTCTGTTTGACCTGTCGATGAACCTCAAAGGCGTCGTAGGTCAGCAACTGAT
CCGCGATAAAAACGGGCAGGGGCGTCACGGTGTGTTTGAAGTGTTGCTCAACAGTCCACGGGTGTCGGACATCATCCGAC
GCGGCGATTTGCATGAGCTGAAAAGCACCATGGCGAAATCGAAAGAGGTTGGCATGCAGACTTTTGATCAGGCGTTGTAT
CAGTTGGTGGTCGAGAACAAGATCAGCGAGCAGGATGCGCTGCACTGTGCCGATTCGGCCAACGATCTGCGCTTGATGCT
CAAGACTCAGCGGGGTGATGTGAGCAGCAGTGGTTCACTGGATCATGTGCAAATCGACATGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.598

100

0.856
  pilU Pseudomonas stutzeri DSM 10701

57.143

95.109

0.543
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44

88.315

0.389
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

40.708

92.12

0.375
  pilT Acinetobacter baumannii D1279779

40.708

92.12

0.375
  pilT Acinetobacter nosocomialis M2

40.708

92.12

0.375
  pilT Pseudomonas aeruginosa PAK

41.194

91.033

0.375
  pilT Pseudomonas stutzeri DSM 10701

39.701

91.033

0.361

Multiple sequence alignment