Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACCP96_RS05190 Genome accession   NZ_CP167226
Coordinates   1242177..1242617 (+) Length   146 a.a.
NCBI ID   WP_218533820.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. fici strain NCPPB 3886     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1237177..1247617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCP96_RS05175 (ACCP96_05175) coaE 1239102..1239710 (-) 609 WP_078517255.1 dephospho-CoA kinase -
  ACCP96_RS05180 (ACCP96_05180) - 1239724..1240587 (-) 864 WP_016849610.1 A24 family peptidase -
  ACCP96_RS05185 (ACCP96_05185) pilC 1240594..1241850 (-) 1257 WP_057685777.1 type II secretion system F family protein Machinery gene
  ACCP96_RS05190 (ACCP96_05190) pilE 1242177..1242617 (+) 441 WP_218533820.1 pilin Machinery gene
  ACCP96_RS05195 (ACCP96_05195) - 1242695..1243129 (+) 435 WP_078590286.1 pilin -
  ACCP96_RS05200 (ACCP96_05200) pilB 1243192..1244928 (+) 1737 WP_122272420.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACCP96_RS05205 (ACCP96_05205) pilB 1245206..1245325 (+) 120 WP_136732873.1 pilus assembly protein Machinery gene

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14991.15 Da        Isoelectric Point: 9.0090

>NTDB_id=1038977 ACCP96_RS05190 WP_218533820.1 1242177..1242617(+) (pilE) [Xanthomonas campestris pv. fici strain NCPPB 3886]
MKKQNGFTLIELMIVVAIIAILAAIALPAYQDYLARSQVSEGLSLASGAKTAVAETYANTGAFPANNAAAGLETAANIKG
KYITSVTVSSGGIITALFNNSNAKLSGKSLVLTPMDNNGSISWGCSNGTTIDQKYLPTSCRTAATP

Nucleotide


Download         Length: 441 bp        

>NTDB_id=1038977 ACCP96_RS05190 WP_218533820.1 1242177..1242617(+) (pilE) [Xanthomonas campestris pv. fici strain NCPPB 3886]
ATGAAGAAGCAAAACGGTTTTACTTTGATCGAACTCATGATCGTGGTCGCGATCATTGCGATCCTGGCTGCCATTGCTCT
GCCGGCTTATCAGGACTACCTCGCGCGTTCGCAGGTCTCTGAAGGTCTGTCGCTGGCGTCGGGTGCAAAGACCGCTGTTG
CTGAGACGTATGCCAATACTGGTGCATTCCCGGCCAACAACGCAGCTGCTGGTCTTGAGACTGCTGCAAACATCAAAGGT
AAGTACATCACCTCGGTGACCGTTAGCTCTGGCGGCATCATCACTGCGCTGTTTAATAACTCCAATGCGAAGTTGAGTGG
TAAGAGCCTTGTCCTGACTCCGATGGACAACAACGGCTCGATCAGCTGGGGTTGCAGCAATGGCACCACCATCGATCAGA
AGTATCTGCCTACCTCTTGCCGTACTGCGGCTACTCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

47.17

100

0.514
  pilE Neisseria gonorrhoeae strain FA1090

46.452

100

0.493
  pilA/pilA1 Eikenella corrodens VA1

45.161

100

0.479
  pilA2 Legionella pneumophila str. Paris

48.571

95.89

0.466
  comP Acinetobacter baylyi ADP1

44.667

100

0.459
  pilA2 Legionella pneumophila strain ERS1305867

47.857

95.89

0.459
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.87

100

0.452
  pilA Acinetobacter baumannii strain A118

42.282

100

0.432
  pilA Ralstonia pseudosolanacearum GMI1000

37.805

100

0.425
  pilA Vibrio cholerae C6706

39.474

100

0.411
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.474

100

0.411
  pilA Vibrio cholerae strain A1552

39.474

100

0.411
  pilA Pseudomonas aeruginosa PAK

38.158

100

0.397
  pilA Vibrio campbellii strain DS40M4

36.913

100

0.377
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.129

95.205

0.363

Multiple sequence alignment