Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB8617_RS10300 Genome accession   NZ_CP166995
Coordinates   2236465..2237499 (+) Length   344 a.a.
NCBI ID   WP_290039388.1    Uniprot ID   -
Organism   Psychromonas sp. GE-S-Ul-11     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2231465..2242499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8617_RS10275 (AB8617_10270) hemW 2232221..2233363 (-) 1143 WP_409438871.1 radical SAM family heme chaperone HemW -
  AB8617_RS10280 (AB8617_10275) - 2233539..2234153 (-) 615 WP_290039392.1 XTP/dITP diphosphatase -
  AB8617_RS10285 (AB8617_10280) - 2234320..2234865 (-) 546 WP_290039391.1 YggT family protein -
  AB8617_RS10290 (AB8617_10285) proC 2234903..2235727 (-) 825 WP_290039390.1 pyrroline-5-carboxylate reductase -
  AB8617_RS10295 (AB8617_10290) - 2235752..2236444 (-) 693 WP_409438872.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB8617_RS10300 (AB8617_10295) pilT 2236465..2237499 (+) 1035 WP_290039388.1 type IV pilus twitching motility protein PilT Machinery gene
  AB8617_RS10305 (AB8617_10300) pilU 2237509..2238618 (+) 1110 WP_290039387.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8617_RS10310 (AB8617_10305) yaaA 2238687..2239463 (+) 777 WP_409438873.1 peroxide stress protein YaaA -
  AB8617_RS10315 (AB8617_10310) - 2239550..2240356 (-) 807 WP_409438874.1 Cof-type HAD-IIB family hydrolase -
  AB8617_RS10320 (AB8617_10315) - 2240344..2241357 (-) 1014 WP_409438875.1 alpha/beta fold hydrolase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38208.02 Da        Isoelectric Point: 6.2882

>NTDB_id=1036648 AB8617_RS10300 WP_290039388.1 2236465..2237499(+) (pilT) [Psychromonas sp. GE-S-Ul-11]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINMPALEHKEVHNLIYDIMSDQQRKDFEELLETDFSFEIKDLAR
FRVNVFNQNRGSAAVFRTIPSEVLTLEALGAPAIFKDISDYPRGLVLVTGPTGSGKSTTLAAMVDYINDSRNEHILTIED
PIEFVHTAKKSLINQREVHKDTLNFEMALRSALREDPDVILIGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTINRI
VDVFPAAEKAMVRSMLSESLRAVISQTLLKKNGGGRIAAHEIMIGIPAIRNLIREDKVAQMYSVIQTGKVHGMQTLDQCL
RDLVAQGFVAYEDAASKAMDPKTF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1036648 AB8617_RS10300 WP_290039388.1 2236465..2237499(+) (pilT) [Psychromonas sp. GE-S-Ul-11]
ATGGATATAACGGAATTATTAGCCTTTAGTGTAAAGCATAAAGCATCAGATCTACACCTTTCTGCAGGTGTTCCTCCAAT
GATTCGTGTGGATGGGGAAGTACGTAAAATAAACATGCCTGCTTTAGAGCATAAAGAAGTGCATAATTTAATTTATGACA
TAATGAGTGATCAGCAACGTAAAGACTTTGAAGAGCTATTAGAAACAGATTTCTCTTTTGAAATTAAAGATCTTGCTCGA
TTTCGTGTGAATGTCTTTAATCAAAATCGTGGCTCTGCTGCGGTATTTCGTACCATCCCTAGTGAAGTGTTAACTTTAGA
AGCACTAGGTGCACCTGCTATTTTTAAAGATATTTCAGATTACCCTCGAGGGTTGGTATTAGTGACAGGCCCAACGGGCT
CAGGTAAATCTACTACATTGGCGGCGATGGTCGATTATATTAATGATTCTCGTAATGAGCATATCTTAACTATCGAAGAC
CCTATTGAGTTTGTACATACAGCTAAAAAATCACTGATCAACCAACGTGAAGTGCATAAAGATACATTAAACTTTGAAAT
GGCGCTACGTAGTGCATTACGTGAAGATCCCGATGTTATTTTGATTGGTGAAATGCGAGACTTAGAAACAATTCGTTTAG
CCTTAACAGCGGCTGAAACAGGTCACTTAGTGTTTGGTACCTTACATACCACTTCAGCTGCAAAAACCATTAACCGTATT
GTTGACGTGTTTCCTGCTGCTGAAAAAGCCATGGTGCGTTCTATGTTATCAGAATCATTACGTGCGGTTATTTCACAAAC
GCTGTTAAAGAAAAATGGCGGTGGTCGTATTGCGGCTCATGAGATTATGATTGGTATTCCAGCTATTCGAAACTTAATTC
GTGAAGATAAAGTGGCGCAAATGTACTCGGTTATACAAACGGGTAAAGTCCACGGAATGCAGACGTTAGACCAATGTTTA
CGAGATTTGGTTGCTCAAGGTTTTGTGGCTTATGAAGATGCAGCTTCAAAAGCGATGGATCCTAAAACATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

78.488

100

0.785
  pilT Acinetobacter baumannii strain A118

78.488

100

0.785
  pilT Acinetobacter nosocomialis M2

78.198

100

0.782
  pilT Pseudomonas aeruginosa PAK

78.198

100

0.782
  pilT Pseudomonas stutzeri DSM 10701

77.035

100

0.77
  pilT Legionella pneumophila strain Lp02

72.965

100

0.73
  pilT Legionella pneumophila strain ERS1305867

72.965

100

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.145

100

0.703
  pilT Vibrio cholerae strain A1552

70.145

100

0.703
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Vibrio cholerae strain A1552

41.593

98.547

0.41
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395

Multiple sequence alignment