Detailed information NTG1036649

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AB8617_RS10305
• Genome accession: NZ_CP166995
• Coordinates: 2237509..2238618 (+)
• Length: 369 a.a.
• NCBI ID: WP_290039387.1
• Uniprot ID: -
• Organism: Psychromonas sp. GE-S-Ul-11
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2232509..2243618

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB8617_RS10280 (AB8617_10275)	-	2233539..2234153 (-)	615	WP_290039392.1	XTP/dITP diphosphatase	-
AB8617_RS10285 (AB8617_10280)	-	2234320..2234865 (-)	546	WP_290039391.1	YggT family protein	-
AB8617_RS10290 (AB8617_10285)	proC	2234903..2235727 (-)	825	WP_290039390.1	pyrroline-5-carboxylate reductase	-
AB8617_RS10295 (AB8617_10290)	-	2235752..2236444 (-)	693	WP_409438872.1	YggS family pyridoxal phosphate-dependent enzyme	-
AB8617_RS10300 (AB8617_10295)	pilT	2236465..2237499 (+)	1035	WP_290039388.1	type IV pilus twitching motility protein PilT	Machinery gene
AB8617_RS10305 (AB8617_10300)	pilU	2237509..2238618 (+)	1110	WP_290039387.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB8617_RS10310 (AB8617_10305)	yaaA	2238687..2239463 (+)	777	WP_409438873.1	peroxide stress protein YaaA	-
AB8617_RS10315 (AB8617_10310)	-	2239550..2240356 (-)	807	WP_409438874.1	Cof-type HAD-IIB family hydrolase	-
AB8617_RS10320 (AB8617_10315)	-	2240344..2241357 (-)	1014	WP_409438875.1	alpha/beta fold hydrolase	-
AB8617_RS10325 (AB8617_10320)	-	2241478..2243193 (-)	1716	WP_290039383.1	proline--tRNA ligase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40596.30 Da
• Isoelectric Point: 6.2872

>NTDB_id=1036649 AB8617_RS10305 WP_290039387.1 2237509..2238618(+) (pilU) [Psychromonas sp. GE-S-Ul-11]
MLDELLSSLDRLKASDLYISVGLPPTVKLNGRLSSLSDDKMTSEDIYNLLARTMDDQGIAQFKSQKEANFALHLEGLGRF
RISAFMQKNNPGMVVRRINDKIPTSDELYLPDQLKEICMAKSGLVLFVGPTGSGKSTTLAAMVGFRNQHASGHILTIEDP
IEFVHEHARSVVTQREVGLDTDSIEEALRNATRQAPDVIVQGEIRSAVSMSHAISHAQTGYLSMATLNANSTDKALEQML
HYIPEERHNQFLNELSVNLKAIVAQRLIPTLDEKGSVAAFEVMVNSPALSNAIRKGERHLLKDIMKASKQAGMQTLDQAL
FKLYSEAKISYSEALAHADSANDLRLMIKLKVGSSSNLASDSLNEFTID

Nucleotide

• Length: 1110 bp

>NTDB_id=1036649 AB8617_RS10305 WP_290039387.1 2237509..2238618(+) (pilU) [Psychromonas sp. GE-S-Ul-11]
ATGCTAGATGAGTTATTAAGTTCTTTAGATCGATTAAAAGCCTCAGACCTTTATATTTCAGTGGGCTTACCACCGACAGT
TAAACTCAATGGGCGTTTATCTTCCCTATCCGATGATAAGATGACATCTGAAGATATTTATAATTTATTAGCGCGTACTA
TGGATGATCAAGGCATCGCTCAGTTTAAAAGCCAAAAAGAAGCTAACTTTGCATTACATCTTGAAGGGCTAGGGCGTTTC
CGTATTAGTGCTTTTATGCAAAAAAATAATCCTGGTATGGTAGTGCGTCGTATTAACGACAAGATTCCAACCAGCGATGA
ATTATACCTTCCAGATCAGCTGAAAGAGATTTGCATGGCTAAGTCTGGTTTAGTGTTATTTGTTGGGCCTACTGGATCTG
GCAAGTCTACAACACTAGCCGCGATGGTGGGGTTTCGTAATCAACATGCATCTGGGCATATATTAACCATTGAAGATCCC
ATTGAGTTTGTGCATGAGCATGCTCGCTCAGTGGTCACGCAGCGTGAAGTGGGATTAGATACTGACTCGATTGAAGAAGC
ATTACGTAATGCGACTCGTCAAGCACCGGATGTGATTGTACAAGGTGAAATTCGTAGTGCGGTGAGCATGTCGCATGCTA
TTAGCCATGCACAAACTGGCTATTTAAGTATGGCAACGCTTAATGCAAATAGTACCGATAAAGCACTGGAGCAGATGCTA
CATTACATACCTGAAGAACGCCATAATCAGTTTTTAAACGAGCTTTCGGTCAATTTAAAAGCAATTGTTGCACAACGTCT
TATTCCGACTTTAGATGAAAAAGGCAGTGTTGCTGCCTTTGAAGTAATGGTTAATTCACCTGCATTATCTAATGCGATTC
GTAAAGGGGAACGACACCTGCTAAAAGATATTATGAAAGCATCTAAACAAGCGGGAATGCAAACCTTAGATCAGGCGTTA
TTTAAATTATATTCAGAAGCAAAAATTAGTTATAGTGAAGCTCTAGCACATGCAGATTCAGCCAATGATCTACGCTTAAT
GATTAAATTAAAAGTTGGGTCAAGTTCTAACCTTGCTTCGGATAGCTTAAATGAATTTACTATCGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (48-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Vibrio cholerae strain A1552	52.989	99.729	0.528
pilU	Pseudomonas stutzeri DSM 10701	52.149	94.58	0.493
pilU	Acinetobacter baylyi ADP1	50.857	94.851	0.482
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.462	95.122	0.366