Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACA097_RS10590 Genome accession   NZ_CP166971
Coordinates   2228537..2229571 (-) Length   344 a.a.
NCBI ID   WP_043248613.1    Uniprot ID   A0A024HBC6
Organism   Pseudomonas sp. QL9     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2223537..2234571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA097_RS10570 (ACA097_10565) - 2224111..2225283 (-) 1173 WP_160285642.1 cystathionine gamma-synthase -
  ACA097_RS10575 (ACA097_10570) - 2225280..2226653 (-) 1374 WP_043248618.1 cystathionine beta-synthase -
  ACA097_RS10580 (ACA097_10575) - 2226798..2227202 (-) 405 WP_043248616.1 TM2 domain-containing protein -
  ACA097_RS10585 (ACA097_10580) pilU 2227302..2228444 (-) 1143 WP_043248615.1 type IV pilus ATPase PilU Machinery gene
  ACA097_RS10590 (ACA097_10585) pilT 2228537..2229571 (-) 1035 WP_043248613.1 type IV pilus twitching motility protein PilT Machinery gene
  ACA097_RS10595 (ACA097_10590) - 2229645..2230334 (+) 690 WP_043248610.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACA097_RS10600 (ACA097_10595) proC 2230352..2231170 (+) 819 WP_371370087.1 pyrroline-5-carboxylate reductase -
  ACA097_RS10605 (ACA097_10600) - 2231181..2231774 (+) 594 WP_043248606.1 YggT family protein -
  ACA097_RS10610 (ACA097_10605) - 2231913..2232209 (+) 297 WP_043248605.1 DUF167 domain-containing protein -
  ACA097_RS10615 (ACA097_10610) - 2232321..2234288 (+) 1968 WP_371370088.1 dynamin family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37855.75 Da        Isoelectric Point: 6.5935

>NTDB_id=1036472 ACA097_RS10590 WP_043248613.1 2228537..2229571(-) (pilT) [Pseudomonas sp. QL9]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGEVFKRIADVPRGLVLVTGPTGSGKSTSLAAMLDYLNNTMYSHILTVED
PIEFVHESKKCLVNQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDMCL
KGLVAKGLVTRDAAKEKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1036472 ACA097_RS10590 WP_043248613.1 2228537..2229571(-) (pilT) [Pseudomonas sp. QL9]
ATGGATATTACCGAGTTGCTCGCCTTCAGCGCCAAGCAAGGCGCTTCGGACCTGCACCTCTCGGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGATGTTCGCCGGATCAACCTGCCGCCCCTCGAGCACAAGCAGGTCCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAATTCCTCGAGACCGACTTCTCCTTCGAAGTCCCTGGCGTCGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCGGGTGCGGTGTTCCGGACCATTCCCTCTAAGGTCCTGAGCATGGA
AGACCTGGGCATGGGCGAGGTGTTCAAGCGCATTGCCGACGTGCCGCGCGGCCTGGTGCTGGTCACCGGTCCGACCGGTT
CGGGCAAGTCGACCTCGCTCGCAGCGATGCTCGACTACCTGAACAACACCATGTACAGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCACGAATCGAAGAAATGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCTCGGCTTCAACGA
GGCGCTGCGCTCGGCCCTGCGTGAGGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTATTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCCGCCGAGGAGAAGTCCATGGTCCGTTCCATGCTCTCCGAATCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTGAAGAAGATCGGCGGCGGCCGTGTCGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGCGCGCTGGGCATGCAGACCCTCGACATGTGCCTC
AAGGGGCTGGTCGCCAAGGGCCTGGTGACCCGCGATGCAGCCAAGGAAAAAGCCAAGATTCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A024HBC6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

94.477

100

0.945
  pilT Pseudomonas stutzeri DSM 10701

93.314

100

0.933
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

81.977

100

0.82
  pilT Legionella pneumophila strain Lp02

76.627

98.256

0.753
  pilT Legionella pneumophila strain ERS1305867

76.627

98.256

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.824

98.837

0.68
  pilT Vibrio cholerae strain A1552

68.824

98.837

0.68
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401

Multiple sequence alignment