Detailed information NTG1036471

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACA097_RS10585
• Genome accession: NZ_CP166971
• Coordinates: 2227302..2228444 (-)
• Length: 380 a.a.
• NCBI ID: WP_043248615.1
• Uniprot ID: A0A024HB19
• Organism: Pseudomonas sp. QL9
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2222302..2233444

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACA097_RS10565 (ACA097_10560)	-	2222719..2223990 (+)	1272	WP_371370086.1	dihydroorotase	-
ACA097_RS10570 (ACA097_10565)	-	2224111..2225283 (-)	1173	WP_160285642.1	cystathionine gamma-synthase	-
ACA097_RS10575 (ACA097_10570)	-	2225280..2226653 (-)	1374	WP_043248618.1	cystathionine beta-synthase	-
ACA097_RS10580 (ACA097_10575)	-	2226798..2227202 (-)	405	WP_043248616.1	TM2 domain-containing protein	-
ACA097_RS10585 (ACA097_10580)	pilU	2227302..2228444 (-)	1143	WP_043248615.1	type IV pilus ATPase PilU	Machinery gene
ACA097_RS10590 (ACA097_10585)	pilT	2228537..2229571 (-)	1035	WP_043248613.1	type IV pilus twitching motility protein PilT	Machinery gene
ACA097_RS10595 (ACA097_10590)	-	2229645..2230334 (+)	690	WP_043248610.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACA097_RS10600 (ACA097_10595)	proC	2230352..2231170 (+)	819	WP_371370087.1	pyrroline-5-carboxylate reductase	-
ACA097_RS10605 (ACA097_10600)	-	2231181..2231774 (+)	594	WP_043248606.1	YggT family protein	-
ACA097_RS10610 (ACA097_10605)	-	2231913..2232209 (+)	297	WP_043248605.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42508.81 Da
• Isoelectric Point: 6.7257

>NTDB_id=1036471 ACA097_RS10585 WP_043248615.1 2227302..2228444(-) (pilU) [Pseudomonas sp. QL9]
MEFEKLLRLMVEKGASDLFVTAGVPPSMKVNGKVLPVTKNPLSPEQTRETVLSVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTIDDLKLPEILKKLAMTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYSQGEITYEDALAYADSANDLRLMIKLGSETDADHLSTMTQGLTLEMDEDPNRRFR

Nucleotide

• Length: 1143 bp

>NTDB_id=1036471 ACA097_RS10585 WP_043248615.1 2227302..2228444(-) (pilU) [Pseudomonas sp. QL9]
ATGGAATTCGAGAAACTTTTACGCCTGATGGTGGAGAAGGGCGCCTCCGACCTTTTCGTCACTGCTGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGGTCCTGCCGGTCACCAAGAACCCACTGTCGCCGGAGCAGACCCGCGAGACCGTGCTGTCGG
TGATGAACGAGCAGCAACGCCGCGACTTCGCCGAGAACCACGAGTGCAACTTCGCGATCAGCGCGCGCGGCATCGGCCGT
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
CGACCTCAAGCTGCCGGAGATCCTCAAGAAGCTGGCCATGACCAAGCGTGGCCTGGTGATCTTCGTCGGCGCCACCGGTA
CCGGTAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAT
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATTGTCACCCAGCGCGAAGTGGGCCTCGACACTGACTCCTTCGAAGT
AGCGTTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTTCGCGCGAGACCATGGACCACG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACGCTGCACGCCAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCCGCCGACCGGCACGGGCAGGTGTGGATGGACCTGTCCCTCAACCTCAAGGCGATCGTCGCCCAACA
ACTGGTCCCGACGCCGGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGATCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTGTACTCCCAGGGCGAGATTACCTACGAAGACGCCCTGGCCTACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTGGGGTCGGAAACCGACGCCGACCACCTGTCGACCATGACCCAGGGCCTGACCCTGGAGATGGACGAAG
ACCCCAACCGCCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A024HB19

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	87.927	100	0.882
pilU	Acinetobacter baylyi ADP1	63.298	98.947	0.626
pilU	Vibrio cholerae strain A1552	57.527	97.895	0.563
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	89.211	0.389