Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACAY00_RS11840 Genome accession   NZ_CP166961
Coordinates   2652423..2653466 (-) Length   347 a.a.
NCBI ID   WP_371373883.1    Uniprot ID   -
Organism   Thalassotalea aquiviva strain 273M-4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2647423..2658466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAY00_RS11820 gshB 2647963..2648922 (-) 960 WP_371373873.1 glutathione synthase -
  ACAY00_RS11825 rsmE 2648954..2649685 (-) 732 WP_371373875.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACAY00_RS11830 metK 2649818..2651005 (-) 1188 WP_371373878.1 methionine adenosyltransferase -
  ACAY00_RS11835 pilU 2651281..2652408 (-) 1128 WP_371373880.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAY00_RS11840 pilT 2652423..2653466 (-) 1044 WP_371373883.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAY00_RS11845 - 2653502..2654185 (+) 684 WP_371373885.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAY00_RS11850 proC 2654247..2655065 (+) 819 WP_371373888.1 pyrroline-5-carboxylate reductase -
  ACAY00_RS11855 - 2655139..2655687 (+) 549 WP_371373890.1 YggT family protein -
  ACAY00_RS11860 - 2655851..2656216 (+) 366 WP_371373893.1 DUF4426 domain-containing protein -
  ACAY00_RS11865 - 2656296..2656889 (+) 594 WP_371373896.1 XTP/dITP diphosphatase -
  ACAY00_RS11870 hemW 2656900..2658048 (+) 1149 WP_371379721.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38732.13 Da        Isoelectric Point: 5.6199

>NTDB_id=1036346 ACAY00_RS11840 WP_371373883.1 2652423..2653466(-) (pilT) [Thalassotalea aquiviva strain 273M-4]
MDITELLAFSVEHNASDLHLSTGMPPAIRVDGDVRKVNVPALEDKEVNALVYDIMTDRQRKEYEENMEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSKILSLDDLGCPDIFRDISDNPRGLVLVTGPTGSGKSTTLAAMVDYINENKRHHILTIED
PIEFVHENKQSLVNQREVHRDTLSFNNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPAEEKSMVRSMLSESLRAVISQALIKKVGGGRVASHEIMIGVPAIRNLIREDKVAQMYSAIQTGMSHGMQTMDQCL
QNLLSRGLITREDAMSKAQDKNQFSSY

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1036346 ACAY00_RS11840 WP_371373883.1 2652423..2653466(-) (pilT) [Thalassotalea aquiviva strain 273M-4]
ATGGACATTACCGAGTTACTAGCATTCAGTGTTGAGCACAATGCATCCGACCTACATTTATCGACGGGTATGCCACCGGC
CATTCGGGTTGATGGTGATGTACGCAAAGTCAATGTACCGGCATTAGAAGACAAAGAAGTCAATGCCTTAGTGTATGACA
TTATGACCGATCGACAACGAAAAGAATACGAAGAAAACATGGAAGTGGATTTCTCTTTCGAAGTACCGAATCTTGCTCGT
TTCAGGGTCAATGCGTTTAACCAAAACCGTGGTCCGGCCGCTGTATTTCGTACCATTCCAAGTAAGATCTTATCATTAGA
TGATTTAGGCTGTCCCGATATATTTCGTGATATTTCCGATAACCCGCGTGGACTTGTGTTAGTTACCGGGCCAACGGGTT
CTGGTAAATCAACGACCCTCGCGGCGATGGTGGATTACATCAATGAAAATAAACGCCACCATATTTTAACCATTGAAGAT
CCTATTGAATTTGTTCACGAAAACAAACAATCACTGGTTAACCAACGTGAAGTGCATCGTGATACCTTAAGTTTTAACAA
CGCCTTGCGTTCGGCCTTACGTGAAGATCCCGATGTTATCCTTGTTGGTGAGATGCGTGACTTGGAAACCATTCGCTTAG
CGATGACCGCCGCCGAAACGGGTCACTTGGTATTTGGTACTTTGCATACAACCTCGGCGCCAAAAACCATAGACCGTATT
GTGGATGTATTCCCAGCAGAAGAAAAATCGATGGTGCGTTCCATGTTATCTGAATCTTTGCGTGCGGTAATATCGCAAGC
CTTGATCAAAAAAGTGGGTGGTGGCCGTGTTGCATCGCATGAAATTATGATCGGGGTTCCAGCGATTCGTAACCTTATTC
GTGAAGATAAAGTGGCACAAATGTATTCAGCCATTCAAACCGGTATGTCGCATGGGATGCAAACGATGGATCAATGCTTA
CAAAACTTGCTTAGCCGAGGGCTTATCACCCGTGAAGATGCGATGAGCAAAGCGCAAGATAAGAATCAGTTCTCGTCGTA
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.616

99.135

0.769
  pilT Pseudomonas aeruginosa PAK

77.035

99.135

0.764
  pilT Acinetobacter nosocomialis M2

76.163

99.135

0.755
  pilT Acinetobacter baumannii D1279779

75.872

99.135

0.752
  pilT Acinetobacter baumannii strain A118

75.872

99.135

0.752
  pilT Acinetobacter baylyi ADP1

75

99.135

0.744
  pilT Legionella pneumophila strain Lp02

72.093

99.135

0.715
  pilT Legionella pneumophila strain ERS1305867

72.093

99.135

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.912

97.695

0.683
  pilT Vibrio cholerae strain A1552

69.912

97.695

0.683
  pilT Neisseria meningitidis 8013

68.497

99.712

0.683
  pilT Neisseria gonorrhoeae MS11

68.208

99.712

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.312

99.712

0.522
  pilU Pseudomonas stutzeri DSM 10701

41.691

98.847

0.412
  pilU Vibrio cholerae strain A1552

41.399

98.847

0.409
  pilU Acinetobacter baylyi ADP1

39.826

99.135

0.395

Multiple sequence alignment