Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACAX20_RS02650 Genome accession   NZ_CP166919
Coordinates   611055..612098 (+) Length   347 a.a.
NCBI ID   WP_371188365.1    Uniprot ID   -
Organism   Thalassotalea maritima strain Sam97     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 606055..617098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAX20_RS02620 hemW 606531..607679 (-) 1149 WP_371188354.1 radical SAM family heme chaperone HemW -
  ACAX20_RS02625 - 607693..608283 (-) 591 WP_371188356.1 XTP/dITP diphosphatase -
  ACAX20_RS02630 - 608367..608810 (-) 444 WP_371188357.1 DUF4426 domain-containing protein -
  ACAX20_RS02635 - 608848..609396 (-) 549 WP_371188359.1 YggT family protein -
  ACAX20_RS02640 proC 609518..610336 (-) 819 WP_371188361.1 pyrroline-5-carboxylate reductase -
  ACAX20_RS02645 - 610336..611016 (-) 681 WP_371188363.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAX20_RS02650 pilT 611055..612098 (+) 1044 WP_371188365.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAX20_RS02655 pilU 612125..613246 (+) 1122 WP_371188367.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAX20_RS02660 - 613369..614043 (-) 675 WP_371188369.1 2OG-Fe(II) oxygenase -
  ACAX20_RS02665 metK 614629..615816 (+) 1188 WP_371188371.1 methionine adenosyltransferase -
  ACAX20_RS02670 rsmE 615975..616703 (+) 729 WP_371188373.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38796.46 Da        Isoelectric Point: 5.8169

>NTDB_id=1036198 ACAX20_RS02650 WP_371188365.1 611055..612098(+) (pilT) [Thalassotalea maritima strain Sam97]
MDITELLAFSVEHNASDLHLSTGMPPAIRVDGDVRKINIPSLEDKDVNALVYDIMTDRQRKEYEENLEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSTILSMEELGCPDIFRDIADNPRGLVLVTGPTGSGKSTTLAAMVDYINQNKRHHILTIED
PIEFVHENKQCLINQREVHRDTLSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPGEEKDMVRSMLSESLKAVISQTLLKKVGGGRVAAHEIMIGIPAIRNLIREDKIAQMYSAIQTGMSHGMQTMDQCL
QNLLSRGLITRENAMAKAVDKNQFRVY

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1036198 ACAX20_RS02650 WP_371188365.1 611055..612098(+) (pilT) [Thalassotalea maritima strain Sam97]
ATGGATATTACCGAGCTTTTAGCATTTAGCGTTGAGCACAATGCTTCCGACTTACATCTTTCTACCGGTATGCCACCAGC
TATTCGTGTCGATGGTGACGTACGTAAGATTAACATTCCGTCGCTCGAAGACAAAGACGTTAATGCTTTAGTCTACGATA
TCATGACAGATCGTCAACGTAAGGAATACGAAGAAAACCTTGAAGTGGACTTTTCATTTGAAGTACCAAACTTGGCACGC
TTCAGGGTAAATGCTTTTAATCAAAACCGTGGTCCAGCAGCGGTATTCCGTACTATCCCTAGCACTATTTTATCAATGGA
AGAGTTAGGCTGCCCTGATATTTTCCGTGATATTGCTGATAACCCTCGCGGCCTAGTGTTGGTTACCGGTCCAACGGGTT
CTGGTAAATCAACAACGTTGGCCGCTATGGTCGATTATATTAACCAAAACAAACGTCATCACATCTTAACCATCGAAGAT
CCGATCGAATTTGTTCACGAAAACAAACAATGTTTGATTAACCAACGTGAAGTTCATCGCGATACGTTAAGCTTTAACGC
AGCACTGCGCAGTGCGCTTCGTGAAGATCCGGATGTGATCTTAGTTGGTGAGATGCGTGACCTTGAAACGATTCGCCTAG
CGATGACAGCGGCAGAAACCGGTCACTTAGTATTTGGTACTTTGCATACCACCTCGGCACCGAAAACCATTGACCGTATT
GTCGATGTATTCCCAGGTGAAGAAAAAGATATGGTACGTTCAATGTTATCTGAGTCGTTAAAAGCGGTTATCTCGCAAAC
CTTATTAAAGAAAGTTGGCGGTGGTCGTGTGGCGGCGCATGAGATTATGATCGGCATTCCAGCAATTCGTAACCTGATCC
GTGAAGATAAAATTGCGCAAATGTATTCAGCTATCCAAACCGGTATGTCACATGGTATGCAGACCATGGATCAGTGTTTA
CAAAATCTATTAAGCCGTGGCCTTATCACCCGTGAAAACGCAATGGCGAAAGCGGTTGATAAAAACCAGTTTAGAGTTTA
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.907

99.135

0.772
  pilT Acinetobacter nosocomialis M2

77.907

99.135

0.772
  pilT Pseudomonas aeruginosa PAK

78.994

97.406

0.769
  pilT Acinetobacter baumannii strain A118

77.616

99.135

0.769
  pilT Acinetobacter baumannii D1279779

77.616

99.135

0.769
  pilT Acinetobacter baylyi ADP1

77.035

99.135

0.764
  pilT Legionella pneumophila strain Lp02

73.837

99.135

0.732
  pilT Legionella pneumophila strain ERS1305867

73.837

99.135

0.732
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.92

97.118

0.689
  pilT Vibrio cholerae strain A1552

70.92

97.118

0.689
  pilT Neisseria meningitidis 8013

69.912

97.695

0.683
  pilT Neisseria gonorrhoeae MS11

69.617

97.695

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.203

99.424

0.539
  pilU Vibrio cholerae strain A1552

42.651

100

0.427
  pilU Pseudomonas stutzeri DSM 10701

40.922

100

0.409
  pilU Acinetobacter baylyi ADP1

39.655

100

0.398

Multiple sequence alignment