Detailed information NTG1036199

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACAX20_RS02655
• Genome accession: NZ_CP166919
• Coordinates: 612125..613246 (+)
• Length: 373 a.a.
• NCBI ID: WP_371188367.1
• Uniprot ID: -
• Organism: Thalassotalea maritima strain Sam97
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 607125..618246

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACAX20_RS02625	-	607693..608283 (-)	591	WP_371188356.1	XTP/dITP diphosphatase	-
ACAX20_RS02630	-	608367..608810 (-)	444	WP_371188357.1	DUF4426 domain-containing protein	-
ACAX20_RS02635	-	608848..609396 (-)	549	WP_371188359.1	YggT family protein	-
ACAX20_RS02640	proC	609518..610336 (-)	819	WP_371188361.1	pyrroline-5-carboxylate reductase	-
ACAX20_RS02645	-	610336..611016 (-)	681	WP_371188363.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACAX20_RS02650	pilT	611055..612098 (+)	1044	WP_371188365.1	type IV pilus twitching motility protein PilT	Machinery gene
ACAX20_RS02655	pilU	612125..613246 (+)	1122	WP_371188367.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACAX20_RS02660	-	613369..614043 (-)	675	WP_371188369.1	2OG-Fe(II) oxygenase	-
ACAX20_RS02665	metK	614629..615816 (+)	1188	WP_371188371.1	methionine adenosyltransferase	-
ACAX20_RS02670	rsmE	615975..616703 (+)	729	WP_371188373.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
ACAX20_RS02675	gshB	616717..617670 (+)	954	WP_371188375.1	glutathione synthase	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 41204.81 Da
• Isoelectric Point: 4.9693

>NTDB_id=1036199 ACAX20_RS02655 WP_371188367.1 612125..613246(+) (pilU) [Thalassotalea maritima strain Sam97]
MYLHTLLESMVNQDGSDMFITVNMNPSAKINGELTAIDDKPLSFDDALELVESSMNDKQKDEFHTSKECNYAIAVDGIGR
FRISAFWQREMPGMVVRRIVTDIPDVDKLGLPSVLKELVMSKRGLVLFVGGTGTGKSTSMASLIGYRNNNARGHILTIED
PVEFVHEHGSSMITQREVGMDTESFESALKSSLRQAPDVILIGEIRSQEIMEHALSFAETGHLCIATLHANNANQAIDRI
MHLVPAEQHQKLQFDLALNLRGIVAQQLVPTRDGQGRRAAIEILLNSPYIAELIKKGHVGEIKEVMSKSREQGMQTFDQA
LFDLYESGDISYADALHHADSPNDLRLMIKLRTNESGGMGGLRGVTLDGDDAE

Nucleotide

• Length: 1122 bp

>NTDB_id=1036199 ACAX20_RS02655 WP_371188367.1 612125..613246(+) (pilU) [Thalassotalea maritima strain Sam97]
ATGTATTTACACACGTTACTCGAGTCGATGGTAAACCAAGATGGTTCGGATATGTTCATTACCGTGAATATGAATCCAAG
TGCCAAAATCAATGGCGAGCTTACCGCTATTGATGACAAACCATTGAGCTTTGACGATGCCTTAGAGCTCGTCGAAAGCT
CTATGAACGACAAGCAAAAAGACGAATTTCATACCAGCAAAGAATGTAACTATGCGATTGCTGTTGACGGTATTGGCCGG
TTTCGTATTTCCGCATTTTGGCAACGAGAAATGCCAGGTATGGTGGTGCGTCGTATCGTCACTGATATTCCCGATGTTGA
TAAGCTAGGCTTGCCATCGGTACTCAAAGAATTGGTGATGTCAAAGCGTGGTTTAGTATTGTTTGTTGGTGGTACAGGTA
CCGGTAAATCAACGTCGATGGCATCACTGATTGGTTATCGTAATAACAATGCTCGAGGACATATATTAACCATTGAAGAT
CCAGTGGAATTTGTCCATGAGCACGGTAGTTCGATGATCACCCAACGTGAAGTTGGTATGGATACGGAGTCGTTTGAATC
GGCTCTGAAAAGTTCACTCCGTCAAGCACCAGATGTGATTCTAATTGGTGAGATCCGCTCACAAGAAATCATGGAACATG
CATTGAGCTTCGCTGAAACCGGACATTTGTGTATTGCGACCCTGCATGCCAACAACGCCAACCAAGCGATTGACCGTATC
ATGCACTTAGTGCCTGCTGAACAGCACCAAAAACTACAGTTTGATTTAGCACTTAACTTACGCGGTATTGTTGCTCAACA
ATTAGTACCAACCCGTGATGGTCAAGGACGCCGAGCGGCGATTGAGATTTTACTCAACTCGCCATACATTGCGGAGTTGA
TCAAAAAAGGGCATGTCGGTGAGATCAAAGAAGTGATGTCAAAATCACGCGAGCAAGGCATGCAAACTTTTGACCAAGCA
TTGTTTGACTTGTATGAGTCTGGTGATATTAGTTACGCTGATGCCTTGCATCATGCTGACTCGCCGAATGATTTACGCTT
AATGATTAAGTTACGCACTAATGAATCGGGCGGTATGGGCGGCTTACGTGGTGTCACTTTAGATGGTGATGACGCGGAGT
AA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.664	95.71	0.571
pilU	Acinetobacter baylyi ADP1	59.091	94.37	0.558
pilU	Vibrio cholerae strain A1552	55.435	98.66	0.547
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.292	95.442	0.394