Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACAY30_RS12270 Genome accession   NZ_CP166871
Coordinates   2813695..2814738 (-) Length   347 a.a.
NCBI ID   WP_290250958.1    Uniprot ID   -
Organism   Thalassotalea ponticola strain KCTC 42155     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2808695..2819738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAY30_RS12250 gshB 2809227..2810183 (-) 957 WP_290250962.1 glutathione synthase -
  ACAY30_RS12255 rsmE 2810207..2810935 (-) 729 WP_290250961.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACAY30_RS12260 metK 2811114..2812304 (-) 1191 WP_290250960.1 methionine adenosyltransferase -
  ACAY30_RS12265 pilU 2812570..2813685 (-) 1116 WP_290250959.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAY30_RS12270 pilT 2813695..2814738 (-) 1044 WP_290250958.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAY30_RS12275 - 2814778..2815449 (+) 672 WP_290250957.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAY30_RS12280 proC 2815453..2816268 (+) 816 WP_290250956.1 pyrroline-5-carboxylate reductase -
  ACAY30_RS12285 - 2816455..2817003 (+) 549 WP_290250955.1 YggT family protein -
  ACAY30_RS12290 - 2817119..2817562 (+) 444 WP_353958580.1 DUF4426 domain-containing protein -
  ACAY30_RS12295 - 2817667..2818266 (+) 600 WP_290250954.1 XTP/dITP diphosphatase -
  ACAY30_RS12300 hemW 2818269..2819417 (+) 1149 WP_290250953.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38749.38 Da        Isoelectric Point: 5.8205

>NTDB_id=1035816 ACAY30_RS12270 WP_290250958.1 2813695..2814738(-) (pilT) [Thalassotalea ponticola strain KCTC 42155]
MDITELLAFSVEHNASDLHLSTGMPPAIRVDGDVRKINIPPLEDKEVNGLVYDIMTDRQRKEYEENLEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSKILSLDELGCPDIFRTIAENPRGLVLVTGPTGSGKSTTLAAMVDYINETKRHHILTIED
PIEFVHDNKQCLINQREVHRDTLSFNNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPGEEKDMVRSMLSESLRAVISQTLIKKNGGGRVAAHEIMVGVPAIRNLIREDKIAQMYSAIQTGMSHGMQTMDQCL
QGLLSRGLITREDAMAKAVDKNQFKVY

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1035816 ACAY30_RS12270 WP_290250958.1 2813695..2814738(-) (pilT) [Thalassotalea ponticola strain KCTC 42155]
ATGGATATTACCGAACTTTTAGCGTTTAGTGTGGAACACAACGCTTCCGATTTACACCTTTCTACTGGCATGCCACCGGC
TATTCGCGTTGATGGTGACGTGCGCAAGATTAACATTCCGCCGTTAGAAGACAAAGAAGTCAATGGCTTGGTCTACGACA
TTATGACCGATCGCCAACGCAAAGAGTACGAAGAAAACTTGGAAGTGGATTTCTCGTTTGAAGTACCAAATCTTGCGCGT
TTCAGGGTTAATGCGTTTAACCAAAACCGAGGTCCAGCAGCGGTATTTCGTACCATCCCTAGTAAAATACTATCACTGGA
CGAACTCGGTTGCCCAGATATTTTCCGCACTATCGCTGAAAACCCACGTGGCTTAGTACTGGTGACAGGCCCAACGGGTT
CGGGTAAATCAACAACACTAGCGGCAATGGTTGATTACATCAACGAAACCAAGCGTCATCACATTCTTACCATTGAAGAT
CCGATAGAATTTGTGCACGACAACAAACAGTGTTTGATTAACCAGCGTGAAGTACATCGCGATACCTTGAGCTTTAACAA
TGCATTGCGCAGTGCACTTCGTGAAGACCCCGACGTTATTCTAGTTGGTGAGATGCGTGACCTTGAAACCATTCGCTTGG
CGATGACTGCGGCAGAAACAGGTCACTTAGTATTTGGTACTTTGCACACCACGTCTGCACCAAAAACCATTGACCGTATT
GTTGATGTATTCCCTGGTGAAGAAAAAGATATGGTGCGCTCGATGCTATCTGAGTCATTGCGCGCAGTTATCTCGCAAAC
ACTGATTAAAAAGAATGGTGGCGGCCGTGTTGCGGCCCACGAGATCATGGTCGGTGTACCGGCGATTCGCAACTTGATTC
GCGAAGATAAGATTGCCCAAATGTACTCAGCTATTCAAACCGGTATGTCACATGGTATGCAAACCATGGATCAGTGTTTA
CAAGGCTTGCTAAGTCGCGGTTTGATCACGCGTGAAGATGCGATGGCCAAAGCGGTAGATAAGAACCAATTTAAAGTGTA
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

78.402

97.406

0.764
  pilT Acinetobacter nosocomialis M2

76.453

99.135

0.758
  pilT Pseudomonas stutzeri DSM 10701

76.453

99.135

0.758
  pilT Acinetobacter baumannii D1279779

76.163

99.135

0.755
  pilT Acinetobacter baumannii strain A118

76.163

99.135

0.755
  pilT Acinetobacter baylyi ADP1

75.581

99.135

0.749
  pilT Legionella pneumophila strain Lp02

72.965

99.135

0.723
  pilT Legionella pneumophila strain ERS1305867

72.965

99.135

0.723
  pilT Neisseria meningitidis 8013

70.501

97.695

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.623

97.118

0.686
  pilT Vibrio cholerae strain A1552

70.623

97.118

0.686
  pilT Neisseria gonorrhoeae MS11

70.206

97.695

0.686
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.913

99.424

0.536
  pilU Vibrio cholerae strain A1552

40.922

100

0.409
  pilU Pseudomonas stutzeri DSM 10701

39.769

100

0.398
  pilU Acinetobacter baylyi ADP1

39.368

100

0.395

Multiple sequence alignment