Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACAY30_RS12265 Genome accession   NZ_CP166871
Coordinates   2812570..2813685 (-) Length   371 a.a.
NCBI ID   WP_290250959.1    Uniprot ID   -
Organism   Thalassotalea ponticola strain KCTC 42155     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2807570..2818685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAY30_RS12240 slyD 2807888..2808370 (+) 483 WP_290250964.1 peptidylprolyl isomerase -
  ACAY30_RS12245 - 2808508..2809062 (-) 555 WP_290250963.1 YqgE/AlgH family protein -
  ACAY30_RS12250 gshB 2809227..2810183 (-) 957 WP_290250962.1 glutathione synthase -
  ACAY30_RS12255 rsmE 2810207..2810935 (-) 729 WP_290250961.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACAY30_RS12260 metK 2811114..2812304 (-) 1191 WP_290250960.1 methionine adenosyltransferase -
  ACAY30_RS12265 pilU 2812570..2813685 (-) 1116 WP_290250959.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAY30_RS12270 pilT 2813695..2814738 (-) 1044 WP_290250958.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAY30_RS12275 - 2814778..2815449 (+) 672 WP_290250957.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAY30_RS12280 proC 2815453..2816268 (+) 816 WP_290250956.1 pyrroline-5-carboxylate reductase -
  ACAY30_RS12285 - 2816455..2817003 (+) 549 WP_290250955.1 YggT family protein -
  ACAY30_RS12290 - 2817119..2817562 (+) 444 WP_353958580.1 DUF4426 domain-containing protein -
  ACAY30_RS12295 - 2817667..2818266 (+) 600 WP_290250954.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41016.90 Da        Isoelectric Point: 5.2705

>NTDB_id=1035815 ACAY30_RS12265 WP_290250959.1 2812570..2813685(-) (pilU) [Thalassotalea ponticola strain KCTC 42155]
MYLHKLLSEMVEQGASDLFITVNLSPSAKINGELTAIDDKVLTYDDALALVLSSMSDKQQQEFEVTKECNYAIAVDNVGR
FRISAFWQREMPGMVIRRIVTDIPDVDKLGLPQVLKDLVMSKRGLVLFVGGTGTGKSTSMASLIGYRNRNARGHILTIED
PVEFVHDHGRSMITQREVGMDTESFDAALKSSLRQAPDVILIGEIRSQEIMEHALSFAETGHLCIATLHANNANQAIDRI
MHLVPAEQHQKLQFDLALNLRGIVAQQLVPTRDGNGRRAAIEILLNSPYIAELIKKGDVGEIKEVMSKSRELGMQTFDQA
LFDLYESGDISYTDALHHADSPNDLRLMIKLRSNDQSGMGGLKGATLDGIE

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=1035815 ACAY30_RS12265 WP_290250959.1 2812570..2813685(-) (pilU) [Thalassotalea ponticola strain KCTC 42155]
ATGTATTTACATAAATTGTTAAGCGAAATGGTTGAACAGGGGGCGTCCGATTTATTCATCACCGTCAACCTATCACCAAG
CGCTAAAATCAATGGCGAATTAACCGCCATTGATGACAAAGTGTTAACTTATGACGATGCCTTAGCACTTGTCCTCAGTT
CGATGAGCGACAAGCAGCAACAGGAATTTGAGGTGACCAAGGAATGTAACTACGCCATTGCGGTAGACAACGTTGGTCGG
TTCCGTATTTCTGCGTTTTGGCAACGCGAAATGCCGGGCATGGTAATACGTCGCATTGTTACCGATATCCCTGATGTTGA
TAAGCTAGGTTTACCGCAAGTGCTAAAAGACTTGGTGATGTCAAAGCGCGGCCTGGTGCTATTTGTCGGTGGTACGGGTA
CCGGTAAGTCAACATCGATGGCGTCGCTTATTGGTTACCGCAATCGCAATGCTCGCGGTCATATTTTAACCATTGAGGAT
CCGGTGGAATTCGTTCACGATCACGGTCGTTCAATGATTACTCAGCGCGAAGTGGGTATGGATACTGAGTCGTTTGATGC
GGCGCTGAAAAGCTCGTTACGTCAAGCGCCCGATGTTATCTTGATTGGTGAAATTCGCTCGCAAGAAATTATGGAGCACG
CATTGAGTTTCGCCGAAACTGGCCACTTGTGTATTGCCACGCTGCATGCCAACAACGCCAACCAAGCGATTGATCGGATT
ATGCATTTGGTACCGGCAGAGCAACACCAAAAACTACAATTTGATTTAGCGTTAAATTTACGCGGGATTGTCGCTCAACA
ATTGGTGCCAACTCGAGATGGCAACGGACGCCGTGCGGCCATCGAAATTTTGTTAAATTCACCGTACATCGCTGAATTGA
TTAAGAAAGGTGATGTTGGCGAAATTAAAGAAGTGATGTCGAAATCTCGTGAATTAGGCATGCAAACCTTTGACCAAGCG
TTGTTTGATTTGTACGAGTCGGGTGACATCAGTTATACCGATGCCTTACATCACGCGGATTCGCCAAATGATCTGCGCTT
GATGATTAAATTGCGCAGCAATGATCAAAGTGGCATGGGTGGCTTAAAAGGGGCAACCCTAGACGGGATTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.49

95.148

0.566
  pilU Acinetobacter baylyi ADP1

59.375

94.879

0.563
  pilU Vibrio cholerae strain A1552

56.522

99.191

0.561
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.135

95.957

0.404
  pilT Acinetobacter nosocomialis M2

39.766

92.183

0.367
  pilT Acinetobacter baumannii D1279779

39.766

92.183

0.367
  pilT Acinetobacter baumannii strain A118

39.766

92.183

0.367
  pilT Pseudomonas aeruginosa PAK

38.841

92.992

0.361

Multiple sequence alignment