Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QUF19_RS14725 Genome accession   NZ_AP028067
Coordinates   3316914..3317951 (-) Length   345 a.a.
NCBI ID   WP_004735478.1    Uniprot ID   A0A1R3EAA6
Organism   Vibrio sp. FE10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3311914..3322951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUF19_RS14695 rsmE 3312587..3313318 (+) 732 WP_286294772.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QUF19_RS14700 gshB 3313328..3314278 (+) 951 WP_004735482.1 glutathione synthase -
  QUF19_RS14705 - 3314395..3314958 (+) 564 WP_286294773.1 YqgE/AlgH family protein -
  QUF19_RS14710 ruvX 3315024..3315446 (+) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  QUF19_RS14715 - 3315545..3315706 (+) 162 WP_170960663.1 hypothetical protein -
  QUF19_RS14720 pilU 3315790..3316899 (-) 1110 WP_286294774.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUF19_RS14725 pilT 3316914..3317951 (-) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  QUF19_RS14730 - 3317978..3318682 (+) 705 WP_286294775.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUF19_RS14735 proC 3318767..3319585 (+) 819 WP_286294776.1 pyrroline-5-carboxylate reductase -
  QUF19_RS14740 - 3319661..3320218 (+) 558 WP_286294777.1 YggT family protein -
  QUF19_RS14745 yggU 3320248..3320538 (+) 291 WP_017101445.1 DUF167 family protein YggU -
  QUF19_RS14750 - 3320567..3320998 (+) 432 WP_286294778.1 DUF4426 domain-containing protein -
  QUF19_RS14755 - 3321047..3321646 (+) 600 WP_286294779.1 XTP/dITP diphosphatase -
  QUF19_RS14760 hemW 3321646..3322833 (+) 1188 WP_286294780.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38317.83 Da        Isoelectric Point: 6.0512

>NTDB_id=103531 QUF19_RS14725 WP_004735478.1 3316914..3317951(-) (pilT) [Vibrio sp. FE10]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPVEIPTLEQLGAPEIFERISNYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=103531 QUF19_RS14725 WP_004735478.1 3316914..3317951(-) (pilT) [Vibrio sp. FE10]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGTCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAGTGATTCACAACGCGGTGAGTTCGAGGAAAAACTGGAAGTCGACTTCTCTTTTGAATTACCCAACGTTGGTCGT
TTCCGTGTTAATGCTTTTAACCAATCTCGTGGCTGCTCTGCTGTATTTCGTACCATTCCAGTCGAGATTCCTACCCTTGA
ACAGCTAGGGGCTCCTGAGATCTTTGAAAGAATATCTAATTACGAAAAAGGTTTAGTCTTGGTCACAGGGCCGACTGGGT
CAGGTAAGTCGACAACTTTGGCAGCGATGGTGGATTACGTTAACCGTAACCATAATAAGCATATATTGACGATTGAAGAT
CCGATCGAATTTGTTCATACCAACAACAAATGCCTAATCAACCAACGAGAAGTACACCGCGATACTCACAGCTTCAAAGC
GGCGTTGCGTAGTGCGTTACGTGAAGATCCAGACGTAATCCTTGTTGGTGAGCTTCGTGACCAAGAGACGATCAGCTTAG
CGTTAACCGCAGCAGAAACAGGCCACTTAGTGTTTGGTACCTTGCACACGAGCTCTGCGGCAAAAACCATAGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGACATGGTGCGTTCAATGTTGTCTGAATCGTTACGTTCGGTGATTGCCCAGAA
GCTGTTAAAGCGTGTTGGTGGCGGTCGTGTGGCTTGTCATGAAATCATGATGGCGACACCTGCGATTAGAAACTTGATCC
GTGAAGACAAGGTCGCGCAGATGTATTCGATAATTCAAACGGGCGCGGCACATGGTATGCAGACCATGGAGCAAAATGCG
AAGCAGCTGATGGCTCAAGGCTTGGTTGATTCAGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R3EAA6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

72.59

96.232

0.699
  pilT Acinetobacter baumannii strain A118

72.59

96.232

0.699
  pilT Acinetobacter nosocomialis M2

72.289

96.232

0.696
  pilT Pseudomonas aeruginosa PAK

69.412

98.551

0.684
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

62.609

100

0.626
  pilT Neisseria gonorrhoeae MS11

62.319

100

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368

Multiple sequence alignment