Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QUF19_RS14720 Genome accession   NZ_AP028067
Coordinates   3315790..3316899 (-) Length   369 a.a.
NCBI ID   WP_286294774.1    Uniprot ID   -
Organism   Vibrio sp. FE10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3310790..3321899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUF19_RS14685 - 3311098..3311598 (+) 501 WP_012604825.1 SprT family zinc-dependent metalloprotease -
  QUF19_RS14690 - 3311696..3312427 (+) 732 WP_065102938.1 endonuclease -
  QUF19_RS14695 rsmE 3312587..3313318 (+) 732 WP_286294772.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QUF19_RS14700 gshB 3313328..3314278 (+) 951 WP_004735482.1 glutathione synthase -
  QUF19_RS14705 - 3314395..3314958 (+) 564 WP_286294773.1 YqgE/AlgH family protein -
  QUF19_RS14710 ruvX 3315024..3315446 (+) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  QUF19_RS14715 - 3315545..3315706 (+) 162 WP_170960663.1 hypothetical protein -
  QUF19_RS14720 pilU 3315790..3316899 (-) 1110 WP_286294774.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUF19_RS14725 pilT 3316914..3317951 (-) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  QUF19_RS14730 - 3317978..3318682 (+) 705 WP_286294775.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUF19_RS14735 proC 3318767..3319585 (+) 819 WP_286294776.1 pyrroline-5-carboxylate reductase -
  QUF19_RS14740 - 3319661..3320218 (+) 558 WP_286294777.1 YggT family protein -
  QUF19_RS14745 yggU 3320248..3320538 (+) 291 WP_017101445.1 DUF167 family protein YggU -
  QUF19_RS14750 - 3320567..3320998 (+) 432 WP_286294778.1 DUF4426 domain-containing protein -
  QUF19_RS14755 - 3321047..3321646 (+) 600 WP_286294779.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41395.26 Da        Isoelectric Point: 5.6716

>NTDB_id=103530 QUF19_RS14720 WP_286294774.1 3315790..3316899(-) (pilU) [Vibrio sp. FE10]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNAAQVGQLLDAMMDQDRRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKATMAKSKEIGMQTFDQALY
DLVVAGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTGALAGVKIDME

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=103530 QUF19_RS14720 WP_286294774.1 3315790..3316899(-) (pilU) [Vibrio sp. FE10]
ATGGAATTGAATCAAATTCTTGAGGGGATGCTATCTCAAAAAGCGTCGGATCTTTATATCACTGTCGATGCGCCTATTTT
ATTTCGTGTCGATGGTGAACTGCGACCTCAAGGCGAGAAATTGAATGCGGCTCAAGTCGGACAGTTGCTTGACGCGATGA
TGGATCAAGACCGACGTGATGAATATCAGCAAACGCGTGAGGCTAATTTTGCCATAGTGCGTGATTTTGGTCGTTTTCGA
GTGAGTGCGTTTTTTCAGCGAGAGCTACCAGGAGCGGTCATTCGTCGTATCGAAACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGATGTACTGCAAGACCTTTCAATCGCAAAGCGTGGACTTGTGCTGGTGGTTGGGGCGACGGGTTCTGGTA
AATCGACTTCAATGGCTGCAATGACGGGTTATCGAAATACTAACCGCTCGGGGCATATCTTGACGGTTGAAGACCCAATT
GAATTTGTTCACGAACATAAAAAGTGCATCGTGACTCAACGTGAGGTTGGGCTCGACACCGAGAGTTATGAAGTTGCGCT
TAAGAACTCTTTGCGCCAAGCTCCTGATATGATCTTGATTGGCGAAATCCGTAGCCGTGAAACCATGGAATATGCGATGA
CCTTTGCTGAAACGGGTCATTTGTGTATGGCAACCTTGCACGCCAATAACGCTAACCAAGCGTTAGAACGTATTCTCCAC
TTGGTGCCTAAAGAGCAGAAAGAACAGTTCTTGTTTGATTTGTCGATGAACCTGCGTGGTGTGGTCGCTCAGCAATTAAT
CCGAGATAAGAATGGTAGCGGTCGTCATGGTGTGTTTGAGATTCTACTCAACAGCCCACGAGTGTCTGACTTGATTCGCC
GTGGTGAGTTACATGAGCTAAAAGCAACCATGGCGAAATCGAAAGAGATTGGGATGCAGACCTTTGACCAAGCTTTGTAT
GATTTGGTCGTTGCTGGCAAGATTAGCGAAGAAGATGCGTTCCACAGTGCAGATTCAGCCAATGACTTGCGCTTAATGCT
AAAAACCAGACGTGGTGATGATGACTACGGGACTGGCGCTTTGGCTGGTGTGAAGATAGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.824

100

0.848
  pilU Pseudomonas stutzeri DSM 10701

56.334

100

0.566
  pilU Acinetobacter baylyi ADP1

54.131

95.122

0.515
  pilT Acinetobacter baumannii strain A118

41.813

92.683

0.388
  pilT Acinetobacter baumannii D1279779

41.813

92.683

0.388
  pilT Acinetobacter nosocomialis M2

41.813

92.683

0.388
  pilT Legionella pneumophila strain ERS1305867

42.183

91.87

0.388
  pilT Legionella pneumophila strain Lp02

42.183

91.87

0.388
  pilT Acinetobacter baylyi ADP1

42.169

89.973

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

87.805

0.379
  pilT Pseudomonas aeruginosa PAK

41.791

90.786

0.379
  pilT Pseudomonas stutzeri DSM 10701

40.299

90.786

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Neisseria meningitidis 8013

39.13

93.496

0.366
  pilT Neisseria gonorrhoeae MS11

39.13

93.496

0.366

Multiple sequence alignment