Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB9K42_RS19795 Genome accession   NZ_CP166747
Coordinates   4042178..4043158 (-) Length   326 a.a.
NCBI ID   WP_370906070.1    Uniprot ID   -
Organism   Citrobacter koseri strain CRE191     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4037178..4048158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9K42_RS19765 (AB9K42_19755) - 4037853..4038350 (+) 498 WP_058668076.1 SprT family zinc-dependent metalloprotease -
  AB9K42_RS19770 (AB9K42_19760) endA 4038445..4039152 (+) 708 WP_012135060.1 deoxyribonuclease I -
  AB9K42_RS19775 (AB9K42_19765) rsmE 4039230..4039961 (+) 732 WP_112001698.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB9K42_RS19780 (AB9K42_19770) gshB 4039974..4040921 (+) 948 WP_012135062.1 glutathione synthase -
  AB9K42_RS19785 (AB9K42_19775) - 4041098..4041661 (+) 564 WP_012135063.1 YqgE/AlgH family protein -
  AB9K42_RS19790 (AB9K42_19780) ruvX 4041661..4042077 (+) 417 WP_049010779.1 Holliday junction resolvase RuvX -
  AB9K42_RS19795 (AB9K42_19785) pilT 4042178..4043158 (-) 981 WP_370906070.1 type IV pilus twitching motility protein PilT Machinery gene
  AB9K42_RS19800 (AB9K42_19790) - 4043176..4043880 (+) 705 WP_112001564.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB9K42_RS19805 (AB9K42_19795) - 4043899..4044465 (+) 567 WP_012135068.1 YggT family protein -
  AB9K42_RS19810 (AB9K42_19800) yggU 4044462..4044752 (+) 291 WP_112001563.1 DUF167 family protein YggU -
  AB9K42_RS19815 (AB9K42_19805) - 4044760..4045353 (+) 594 WP_012135070.1 XTP/dITP diphosphatase -
  AB9K42_RS19820 (AB9K42_19810) hemW 4045346..4046482 (+) 1137 WP_047461345.1 radical SAM family heme chaperone HemW -
  AB9K42_RS19825 (AB9K42_19815) ansB 4046601..4047647 (-) 1047 WP_012135072.1 L-asparaginase 2 -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35680.74 Da        Isoelectric Point: 6.8793

>NTDB_id=1035138 AB9K42_RS19795 WP_370906070.1 4042178..4043158(-) (pilT) [Citrobacter koseri strain CRE191]
MDMEEIVALSVKHNVSDLHLCNAWPARWRKRGKVEIAPFSSPNVEVLLSQWLNDAQRRAWQADGQLDFAVSLPGELRLRA
SAFHHEQGASLALRLLPTTCPQLDALQTPAVLPALLGGENGLILVTGATGSGKSTTLAAMVEYLNQHLDGHILTLEDPIE
YRHTSQRCLIQQREVGVHCRSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRSQLAGSLRAVLSQKLETDRQDGRVALFELLINTPAVGNLIREGKIHQLPGVIQTSQQAGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1035138 AB9K42_RS19795 WP_370906070.1 4042178..4043158(-) (pilT) [Citrobacter koseri strain CRE191]
ATGGATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAACGCATGGCCTGCGCG
CTGGCGTAAACGGGGAAAAGTGGAAATAGCCCCGTTTAGCAGCCCGAATGTCGAGGTTTTGCTGTCACAATGGCTTAACG
ACGCGCAGCGCCGGGCGTGGCAGGCGGATGGTCAGCTCGATTTTGCGGTGTCGCTGCCGGGCGAACTGCGGTTACGCGCC
AGCGCTTTTCATCACGAGCAGGGCGCATCGCTGGCGCTGCGGTTGCTGCCCACTACGTGCCCGCAACTGGACGCGCTCCA
GACGCCTGCCGTGCTACCCGCGCTCCTTGGCGGCGAGAACGGACTGATTCTGGTGACCGGGGCGACGGGAAGCGGGAAAT
CCACCACGCTGGCGGCAATGGTGGAATACCTCAACCAGCATCTCGATGGTCATATTCTGACGCTGGAAGATCCTATTGAG
TACCGCCATACCAGCCAGCGCTGCCTGATTCAGCAGCGGGAGGTTGGCGTACACTGCCGCTCCTTTGCCGCCGGGTTACG
CGCTGCGCTGCGTGAAGACCCGGATGTGATTTTATTAGGCGAACTGCGCGACAGCGAGACTATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTATTGGCGACGCTACATACGCGCGGCGCGGCACAGGCGGTAGAGCGGCTGGTCGACTCC
TTTCCGGCGCAGGAGAAAGATCCGGTACGCAGTCAACTTGCAGGCAGCCTGCGCGCCGTGTTGTCGCAGAAGCTGGAAAC
CGACAGGCAGGACGGACGCGTGGCGTTATTCGAACTGCTCATCAACACGCCAGCGGTTGGCAATCTTATTCGGGAGGGGA
AAATTCACCAGTTGCCCGGCGTTATTCAGACCAGCCAGCAGGCGGGGATGCAGACGTTTGCCCAGAGTCTGCAACAGCGG
CAGGCGCAGGGCAGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(53-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.07

100

0.512
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.07

100

0.512
  pilT Neisseria meningitidis 8013

49.39

100

0.497
  pilT Neisseria gonorrhoeae MS11

49.085

100

0.494
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Legionella pneumophila strain ERS1305867

46.584

98.773

0.46
  pilT Legionella pneumophila strain Lp02

46.584

98.773

0.46
  pilU Pseudomonas stutzeri DSM 10701

41.212

100

0.417
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.062

98.773

0.396
  pilU Acinetobacter baylyi ADP1

38.58

99.387

0.383

Multiple sequence alignment