Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QUE86_RS10935 Genome accession   NZ_AP028065
Coordinates   2423867..2424907 (+) Length   346 a.a.
NCBI ID   WP_130049642.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. FE4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2418867..2429907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE86_RS10905 hemW 2419521..2420654 (-) 1134 WP_130049647.1 radical SAM family heme chaperone HemW -
  QUE86_RS10910 - 2420651..2421250 (-) 600 WP_250661690.1 XTP/dITP diphosphatase -
  QUE86_RS10915 - 2421258..2421698 (-) 441 WP_178926612.1 DUF4426 domain-containing protein -
  QUE86_RS10920 - 2421757..2422290 (-) 534 WP_130049645.1 YggT family protein -
  QUE86_RS10925 proC 2422312..2423133 (-) 822 WP_130049644.1 pyrroline-5-carboxylate reductase -
  QUE86_RS10930 - 2423151..2423864 (-) 714 WP_130049643.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUE86_RS10935 pilT 2423867..2424907 (+) 1041 WP_130049642.1 type IV pilus twitching motility protein PilT Machinery gene
  QUE86_RS10940 pilU 2424922..2426055 (+) 1134 WP_286257177.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUE86_RS10945 - 2426056..2426928 (+) 873 WP_286257179.1 hypothetical protein -
  QUE86_RS10950 ruvX 2426925..2427374 (-) 450 WP_130049639.1 Holliday junction resolvase RuvX -
  QUE86_RS10955 - 2427394..2427951 (-) 558 WP_130049638.1 YqgE/AlgH family protein -
  QUE86_RS10960 gshB 2427976..2428929 (-) 954 WP_130049637.1 glutathione synthase -
  QUE86_RS10965 rsmE 2428998..2429732 (-) 735 WP_286257182.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38259.51 Da        Isoelectric Point: 6.1844

>NTDB_id=103493 QUE86_RS10935 WP_130049642.1 2423867..2424907(+) (pilT) [Pseudoalteromonas sp. FE4]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRINIPALEQKDVNSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSDVLTLDDLGAPDIFKTISDNPRGLVLVTGPTGSGKSTTLAAMVDYINQHKHHHILTIED
PIEFVHENKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKDMVRSMLSESLRAVISQTLLKKVGGGRVAAHEIMIGIPAIRNLIREDKIAQMYSSIQTGASHGMQTMDQCL
ASLVNFGIITTNDARAKAQDKTQFGG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=103493 QUE86_RS10935 WP_130049642.1 2423867..2424907(+) (pilT) [Pseudoalteromonas sp. FE4]
ATGGATATTACCGAATTATTGGCCTTTAGTGTGCAACACAAAGCCTCGGATTTACACTTGTCATCGGGCGTATCACCTAT
GATACGTGTTGATGGTGATGTGCGTCGTATTAATATTCCAGCATTAGAACAAAAAGATGTAAATAGTCTGGTATACGATA
TTATGAACGATAATCAGCGTAAGGACTACGAACAAAATCTCGAAGTGGATTTTTCTTTTGAAGTACCAAACCTTGCGCGT
TTTCGTGTAAACGCATTTAACTCAAACCGTGGCCCAGCGGCGGTGTTTCGTACTATCCCAAGTGATGTTTTAACGCTTGA
TGACTTAGGTGCGCCTGATATTTTTAAAACCATTTCCGATAATCCGCGTGGTTTAGTGTTGGTTACAGGCCCGACTGGTT
CTGGTAAATCAACTACCTTAGCGGCGATGGTTGATTATATTAATCAACATAAGCATCACCATATTTTAACTATCGAAGAT
CCTATCGAATTCGTGCACGAGAATAAGCTGAGTCTCATTAACCAACGCGAAGTTCACCGTGACACGCATAGCTTCTCTAA
TGCACTTAGAAGCGCATTACGTGAAGATCCGGATGTAATCTTGGTGGGTGAGCTTCGTGACCTTGAAACAATTCGCTTAG
CGATGACCGCGGCAGAAACAGGTCACTTGGTGTTTGGTACCTTGCATACTACTTCTGCACCAAAAACTATCGACCGTATC
ATTGACGTATTCCCTGGTGAAGAAAAAGACATGGTGCGTTCAATGTTGTCTGAATCACTACGTGCCGTAATATCACAAAC
CTTACTTAAAAAAGTGGGCGGTGGCCGAGTAGCAGCCCATGAAATCATGATAGGTATTCCTGCTATCCGAAACCTAATCC
GAGAAGATAAAATCGCGCAAATGTATTCATCAATTCAAACCGGTGCGTCCCATGGAATGCAGACCATGGATCAATGTTTA
GCAAGCTTAGTTAACTTCGGTATTATTACAACCAATGATGCGCGTGCTAAAGCACAGGATAAAACCCAGTTTGGTGGTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.907

99.422

0.775
  pilT Acinetobacter baumannii strain A118

77.907

99.422

0.775
  pilT Acinetobacter baylyi ADP1

77.907

99.422

0.775
  pilT Acinetobacter baumannii D1279779

77.907

99.422

0.775
  pilT Pseudomonas stutzeri DSM 10701

77.035

99.422

0.766
  pilT Pseudomonas aeruginosa PAK

76.453

99.422

0.76
  pilT Legionella pneumophila strain ERS1305867

72.384

99.422

0.72
  pilT Legionella pneumophila strain Lp02

72.384

99.422

0.72
  pilT Neisseria meningitidis 8013

70.968

98.555

0.699
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.386

97.977

0.699
  pilT Vibrio cholerae strain A1552

71.386

97.977

0.699
  pilT Neisseria gonorrhoeae MS11

70.674

98.555

0.697
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.711

0.52
  pilU Vibrio cholerae strain A1552

42.388

96.821

0.41
  pilU Acinetobacter baylyi ADP1

39.706

98.266

0.39
  pilU Pseudomonas stutzeri DSM 10701

39.701

96.821

0.384

Multiple sequence alignment