Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QUE86_RS10940 Genome accession   NZ_AP028065
Coordinates   2424922..2426055 (+) Length   377 a.a.
NCBI ID   WP_286257177.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. FE4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2419922..2431055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE86_RS10910 - 2420651..2421250 (-) 600 WP_250661690.1 XTP/dITP diphosphatase -
  QUE86_RS10915 - 2421258..2421698 (-) 441 WP_178926612.1 DUF4426 domain-containing protein -
  QUE86_RS10920 - 2421757..2422290 (-) 534 WP_130049645.1 YggT family protein -
  QUE86_RS10925 proC 2422312..2423133 (-) 822 WP_130049644.1 pyrroline-5-carboxylate reductase -
  QUE86_RS10930 - 2423151..2423864 (-) 714 WP_130049643.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUE86_RS10935 pilT 2423867..2424907 (+) 1041 WP_130049642.1 type IV pilus twitching motility protein PilT Machinery gene
  QUE86_RS10940 pilU 2424922..2426055 (+) 1134 WP_286257177.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUE86_RS10945 - 2426056..2426928 (+) 873 WP_286257179.1 hypothetical protein -
  QUE86_RS10950 ruvX 2426925..2427374 (-) 450 WP_130049639.1 Holliday junction resolvase RuvX -
  QUE86_RS10955 - 2427394..2427951 (-) 558 WP_130049638.1 YqgE/AlgH family protein -
  QUE86_RS10960 gshB 2427976..2428929 (-) 954 WP_130049637.1 glutathione synthase -
  QUE86_RS10965 rsmE 2428998..2429732 (-) 735 WP_286257182.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QUE86_RS10970 - 2429802..2430263 (-) 462 WP_130049635.1 hypothetical protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41844.83 Da        Isoelectric Point: 6.2382

>NTDB_id=103494 QUE86_RS10940 WP_286257177.1 2424922..2426055(+) (pilU) [Pseudoalteromonas sp. FE4]
MSLPLNHFLQVMIDKKASDLFVSSQLPVSAKINGELVHLTDEKLSDEASLDLVESAMSDKQRKEFHDTKECNFAIATDEG
RFRVSAFWQRDRAGMVIRRIVTQIPDVNELGLPSTLTDVIMAKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIE
DPIEFVHEHRKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIVAQQLIPTADGEGRVAAIEILLNSPMVAELIKKGDVGSIKETMAKSKEMGMQTFDQ
ALFELYKQRRINYADALHHADSPNDLRLMIKLQNNEQSGAGFLQGVTIDGLDGKSDT

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=103494 QUE86_RS10940 WP_286257177.1 2424922..2426055(+) (pilU) [Pseudoalteromonas sp. FE4]
ATGTCTTTACCTTTAAATCATTTTTTACAAGTTATGATCGACAAAAAAGCGTCTGACTTGTTTGTTTCTAGCCAACTGCC
TGTCAGTGCAAAAATAAACGGTGAGCTAGTACATCTTACCGACGAAAAGCTTTCTGATGAAGCTTCACTCGATTTGGTTG
AGTCAGCCATGAGCGATAAGCAGCGTAAAGAATTTCATGATACTAAAGAGTGTAACTTTGCGATTGCAACTGATGAGGGC
CGTTTCCGTGTTTCTGCTTTTTGGCAGCGTGACAGAGCTGGTATGGTTATTCGTCGTATCGTGACTCAGATACCTGATGT
GAATGAGCTAGGGTTGCCGTCAACCTTAACCGATGTGATTATGGCTAAACGTGGTTTAGTTCTGTTTGTGGGTGGTACAG
GTACGGGTAAATCGACCTCGCTCGCTGCGCTTTTAGGTTATCGTAACCGCAACCAGCGAGGTCATATTTTAACGATTGAA
GATCCTATTGAATTTGTTCACGAGCATCGTAAAAGTATCATTACTCAACGTGAAGTAGGTTTAGATACAGAAAGCTTCGA
ATCAGCGCTAAAAAGCTCGTTGCGTCAAGCGCCTGATGTAATCTTAATTGGTGAAATACGCTCACAAGAAACCATGGAGT
ATGCATTAAGCTTTGCAGAAACAGGTCACTTGTGTGTGGCAACACTGCATGCGAATAATGCTAACCAAGCAATCGACCGT
ATTATGCACTTAGTACCTAAAGAAAAGCACGATAAGTTAAAATACGACTTAGCACTTAACCTTCGCGCTATTGTTGCTCA
GCAGTTGATTCCAACTGCCGATGGCGAAGGACGTGTAGCTGCTATCGAAATTTTGCTAAATTCACCTATGGTGGCAGAGC
TTATTAAAAAAGGTGATGTTGGTTCTATCAAAGAAACCATGGCGAAATCGAAAGAAATGGGTATGCAGACCTTTGATCAA
GCATTGTTTGAGCTTTATAAGCAGCGTCGCATTAACTACGCCGATGCGTTGCATCATGCTGATTCGCCAAATGACCTACG
CTTAATGATTAAGCTACAAAACAATGAGCAAAGTGGCGCAGGCTTTTTACAAGGCGTGACTATCGATGGTCTTGATGGAA
AGAGTGACACATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

57.493

97.347

0.56
  pilU Pseudomonas stutzeri DSM 10701

59.538

91.777

0.546
  pilU Acinetobacter baylyi ADP1

58.239

93.369

0.544
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.855

94.96

0.416
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366

Multiple sequence alignment