Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABK042_RS11430 Genome accession   NZ_CP166454
Coordinates   2394637..2395674 (-) Length   345 a.a.
NCBI ID   WP_004147099.1    Uniprot ID   A0AAP7J2J0
Organism   Stenotrophomonas maltophilia strain 1945B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2389637..2400674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABK042_RS11405 (ABK042_11400) - 2389924..2390133 (-) 210 WP_005412490.1 DUF4287 domain-containing protein -
  ABK042_RS11410 (ABK042_11405) - 2390227..2391126 (+) 900 WP_049454184.1 LysR family transcriptional regulator -
  ABK042_RS11415 (ABK042_11410) - 2391174..2392157 (-) 984 WP_049454182.1 aldo/keto reductase -
  ABK042_RS11420 (ABK042_11415) - 2392369..2393313 (+) 945 WP_049396168.1 IS481-like element ISStma12 family transposase -
  ABK042_RS11425 (ABK042_11420) pilU 2393394..2394488 (-) 1095 WP_043033789.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABK042_RS11430 (ABK042_11425) pilT 2394637..2395674 (-) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  ABK042_RS11435 (ABK042_11430) - 2395762..2396439 (+) 678 WP_049453897.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABK042_RS11440 (ABK042_11435) proC 2396458..2397279 (+) 822 WP_049453896.1 pyrroline-5-carboxylate reductase -
  ABK042_RS11445 (ABK042_11440) - 2397339..2398214 (-) 876 WP_005412485.1 LysR family transcriptional regulator -
  ABK042_RS11450 (ABK042_11445) - 2398313..2398915 (+) 603 WP_033834829.1 short chain dehydrogenase -
  ABK042_RS11455 (ABK042_11450) soxR 2398988..2399422 (-) 435 WP_033834828.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38658.41 Da        Isoelectric Point: 6.6654

>NTDB_id=1034547 ABK042_RS11430 WP_004147099.1 2394637..2395674(-) (pilT) [Stenotrophomonas maltophilia strain 1945B]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1034547 ABK042_RS11430 WP_004147099.1 2394637..2395674(-) (pilT) [Stenotrophomonas maltophilia strain 1945B]
ATGGATATCGCCGAACTGCTGGCGTTTTCCGTTAAGAACAAAGCGTCCGACCTGCACCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTTCGCCGGATCAACATCCCAGCCCTGGACCACAAGCAGGTCCATGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTGGACTTCTCCTTCGAGATTCCATCGCTGGCGCGC
TTCCGAGTGAACGCGTTCAACCAGAACCGCGGTGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAGGTGCTCACCCTCGA
GGACCTGGCCTGCCCGCCGCTGTTCCGCGAAGTGATCCAGCAGCCGCAGGGCCTGATCCTTGTGACCGGTCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCAGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCATACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCATGGCTTCAATGA
AGCACTGCGCTCGGCGCTGCGTGAGGACCCGGACATCATCCTGGTGGGCGAACTTCGTGACCTGGAAACCATCCGCCTGG
CACTGACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGTTGCGCGCGGTCATTTCGCAGGC
GCTGCTGAAGAAGGTTGGCGGCGGTCGTACCGCAGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACTGGCCAGCAATACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGAAACCAGGCCCGTGAGTACGCCAAGGACAAGCGCCTGTTCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391

Multiple sequence alignment