Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABK042_RS11425 Genome accession   NZ_CP166454
Coordinates   2393394..2394488 (-) Length   364 a.a.
NCBI ID   WP_043033789.1    Uniprot ID   A0A0X3QW38
Organism   Stenotrophomonas maltophilia strain 1945B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2388394..2399488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABK042_RS11395 (ABK042_11390) - 2388477..2389022 (+) 546 WP_033834834.1 DNA-3-methyladenine glycosylase I -
  ABK042_RS11400 (ABK042_11395) - 2389106..2389813 (-) 708 WP_014036228.1 YitT family protein -
  ABK042_RS11405 (ABK042_11400) - 2389924..2390133 (-) 210 WP_005412490.1 DUF4287 domain-containing protein -
  ABK042_RS11410 (ABK042_11405) - 2390227..2391126 (+) 900 WP_049454184.1 LysR family transcriptional regulator -
  ABK042_RS11415 (ABK042_11410) - 2391174..2392157 (-) 984 WP_049454182.1 aldo/keto reductase -
  ABK042_RS11420 (ABK042_11415) - 2392369..2393313 (+) 945 WP_049396168.1 IS481-like element ISStma12 family transposase -
  ABK042_RS11425 (ABK042_11420) pilU 2393394..2394488 (-) 1095 WP_043033789.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABK042_RS11430 (ABK042_11425) pilT 2394637..2395674 (-) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  ABK042_RS11435 (ABK042_11430) - 2395762..2396439 (+) 678 WP_049453897.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABK042_RS11440 (ABK042_11435) proC 2396458..2397279 (+) 822 WP_049453896.1 pyrroline-5-carboxylate reductase -
  ABK042_RS11445 (ABK042_11440) - 2397339..2398214 (-) 876 WP_005412485.1 LysR family transcriptional regulator -
  ABK042_RS11450 (ABK042_11445) - 2398313..2398915 (+) 603 WP_033834829.1 short chain dehydrogenase -
  ABK042_RS11455 (ABK042_11450) soxR 2398988..2399422 (-) 435 WP_033834828.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40569.61 Da        Isoelectric Point: 6.7680

>NTDB_id=1034546 ABK042_RS11425 WP_043033789.1 2393394..2394488(-) (pilU) [Stenotrophomonas maltophilia strain 1945B]
MAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRIVNFFPEDRR
NQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=1034546 ABK042_RS11425 WP_043033789.1 2393394..2394488(-) (pilU) [Stenotrophomonas maltophilia strain 1945B]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACTGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCC
GATCACGCAGACCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAAT
TCGAGAAGACCCACGAATGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGC
AACCAGGTCGGCATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACGGTGGAAGAACTGAGCCTGCCGCCGATCATCAA
GACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGTGCCACCGGTACCGGTAAATCGACCTCGCTGGCGGCGA
TGATCGGTTACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGATCCGATCGAATTCGTGCACAAGCACGAG
GGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGC
GCCGGACGTGATCATGATTGGCGAGGTGCGTACCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACC
TGGTGCTGTGCACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGC
AACCAGCTGCTGATGGACCTGTCGCTGAATCTCAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTC
GCGCAAGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGA
AGGAAGTGATGAAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAG
ATCAGCTACGAGGACGCGCTGCGTTACGCCGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGA
CGCACGCACGCTGTCACAGGGCCTGGATGGCGTGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(106-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.715

98.352

0.676
  pilU Acinetobacter baylyi ADP1

64.08

95.604

0.613
  pilU Vibrio cholerae strain A1552

54.467

95.33

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.899

94.78

0.407
  pilT Acinetobacter baumannii strain A118

40.303

90.659

0.365
  pilT Acinetobacter nosocomialis M2

40.303

90.659

0.365
  pilT Acinetobacter baumannii D1279779

40.303

90.659

0.365
  pilT Pseudomonas aeruginosa PAK

40.615

89.286

0.363

Multiple sequence alignment