Detailed information NTG1034546

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABK042_RS11425
• Genome accession: NZ_CP166454
• Coordinates: 2393394..2394488 (-)
• Length: 364 a.a.
• NCBI ID: WP_043033789.1
• Uniprot ID: A0A0X3QW38
• Organism: Stenotrophomonas maltophilia strain 1945B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	2392369..2393313	2393394..2394488	flank	81

Gene organization within MGE regions

Location: 2392369..2394488

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABK042_RS11420 (ABK042_11415)	-	2392369..2393313 (+)	945	WP_049396168.1	IS481-like element ISStma12 family transposase	-
ABK042_RS11425 (ABK042_11420)	pilU	2393394..2394488 (-)	1095	WP_043033789.1	PilT/PilU family type 4a pilus ATPase	Machinery gene

Sequence

Protein

• Length: 364 a.a.
• Molecular weight: 40569.61 Da
• Isoelectric Point: 6.7680

>NTDB_id=1034546 ABK042_RS11425 WP_043033789.1 2393394..2394488(-) (pilU) [Stenotrophomonas maltophilia strain 1945B]
MAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRIVNFFPEDRR
NQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide

• Length: 1095 bp

>NTDB_id=1034546 ABK042_RS11425 WP_043033789.1 2393394..2394488(-) (pilU) [Stenotrophomonas maltophilia strain 1945B]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACTGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCC
GATCACGCAGACCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAAT
TCGAGAAGACCCACGAATGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGC
AACCAGGTCGGCATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACGGTGGAAGAACTGAGCCTGCCGCCGATCATCAA
GACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGTGCCACCGGTACCGGTAAATCGACCTCGCTGGCGGCGA
TGATCGGTTACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGATCCGATCGAATTCGTGCACAAGCACGAG
GGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGC
GCCGGACGTGATCATGATTGGCGAGGTGCGTACCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACC
TGGTGCTGTGCACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGC
AACCAGCTGCTGATGGACCTGTCGCTGAATCTCAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTC
GCGCAAGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGA
AGGAAGTGATGAAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAG
ATCAGCTACGAGGACGCGCTGCGTTACGCCGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGA
CGCACGCACGCTGTCACAGGGCCTGGATGGCGTGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (106-261)

3D structure

• AlphaFold DB: A0A0X3QW38

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	68.715	98.352	0.676
pilU	Acinetobacter baylyi ADP1	64.08	95.604	0.613
pilU	Vibrio cholerae strain A1552	54.467	95.33	0.519
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.899	94.78	0.407
pilT	Acinetobacter baumannii strain A118	40.303	90.659	0.365
pilT	Acinetobacter nosocomialis M2	40.303	90.659	0.365
pilT	Acinetobacter baumannii D1279779	40.303	90.659	0.365
pilT	Pseudomonas aeruginosa PAK	40.615	89.286	0.363