Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB9R84_RS12140 Genome accession   NZ_CP166304
Coordinates   2531429..2532463 (-) Length   344 a.a.
NCBI ID   WP_317074630.1    Uniprot ID   -
Organism   Oceanimonas smirnovii strain OS2020-133     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2526429..2537463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9R84_RS12120 (AB9R84_12110) - 2527312..2528019 (-) 708 WP_418139400.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  AB9R84_RS12125 (AB9R84_12115) srmB 2528090..2529391 (+) 1302 WP_418139401.1 ATP-dependent RNA helicase SrmB -
  AB9R84_RS12130 (AB9R84_12120) yaaA 2529516..2530295 (-) 780 WP_395535141.1 peroxide stress protein YaaA -
  AB9R84_RS12135 (AB9R84_12125) pilU 2530303..2531412 (-) 1110 WP_317074629.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB9R84_RS12140 (AB9R84_12130) pilT 2531429..2532463 (-) 1035 WP_317074630.1 type IV pilus twitching motility protein PilT Machinery gene
  AB9R84_RS12145 (AB9R84_12135) - 2532504..2533196 (+) 693 WP_317074631.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB9R84_RS12150 (AB9R84_12140) proC 2533207..2534031 (+) 825 WP_395535140.1 pyrroline-5-carboxylate reductase -
  AB9R84_RS12155 (AB9R84_12145) - 2534041..2534592 (+) 552 WP_395535139.1 YggT family protein -
  AB9R84_RS12160 (AB9R84_12150) - 2534661..2535074 (+) 414 WP_395535138.1 DUF4426 domain-containing protein -
  AB9R84_RS12165 (AB9R84_12155) rdgB 2535085..2535690 (+) 606 WP_019933100.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  AB9R84_RS12170 (AB9R84_12160) hemW 2535684..2536829 (+) 1146 WP_395535137.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38305.82 Da        Isoelectric Point: 6.7435

>NTDB_id=1034146 AB9R84_RS12140 WP_317074630.1 2531429..2532463(-) (pilT) [Oceanimonas smirnovii strain OS2020-133]
MDITELLAFSVKHNASDLHLSSGVQPLLRVDGDIRKINLPVLDHREVHRLVYDIMNDRQRKELEENFEVDFSFELPGLAR
FRVNAYHQARGAAAVFRVIPSDMWTLEQLGGPDIFERIAEYSRGLVLVTGPTGSGKSTTLAAMVNHINDNFNKHILTIED
PIEFVHDNKKCLVNQREVHRDTHSFSNALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQNLLKKVNGGRVAAHEIMLGTPAIRNLIREDKVAQMYSVIQTGMAHGMQTMDQSL
KRLVASGQVAPADARLRAADPQSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1034146 AB9R84_RS12140 WP_317074630.1 2531429..2532463(-) (pilT) [Oceanimonas smirnovii strain OS2020-133]
ATGGATATTACGGAATTACTGGCGTTCAGTGTAAAGCATAATGCCTCGGATCTGCATCTCTCTTCCGGTGTGCAGCCACT
GCTGCGGGTGGATGGCGATATTCGCAAAATTAATTTGCCGGTGCTGGATCACAGAGAAGTGCACCGGCTGGTCTACGACA
TCATGAATGATCGCCAGCGCAAGGAGCTGGAAGAAAACTTTGAAGTCGATTTTTCCTTTGAGCTGCCGGGGCTGGCGCGT
TTTCGGGTAAATGCCTATCATCAGGCGCGCGGGGCGGCGGCGGTGTTCCGGGTGATCCCCAGCGACATGTGGACCCTGGA
GCAACTGGGCGGACCGGACATCTTTGAGCGCATTGCCGAGTATTCCCGTGGTCTGGTGCTGGTGACCGGCCCCACCGGCT
CGGGCAAGTCCACCACGCTGGCAGCCATGGTCAATCACATCAATGACAACTTCAACAAGCATATTCTGACCATTGAAGAT
CCCATTGAATTTGTGCACGACAACAAGAAATGCCTGGTCAACCAGCGTGAAGTGCACAGAGACACCCATTCTTTCAGTAA
TGCCCTGCGCTCGGCGCTGCGGGAAGACCCGGATATTATTCTGGTGGGAGAGCTGCGGGATCTCGAGACCATTCGGCTGG
CACTCACTGCCGCCGAAACCGGCCATCTGGTGTTTGGTACCCTGCATACCTCGTCGGCGGCCAAGACCATTGACCGTATT
ATTGATGTGTTTCCCGGTGCGGAAAAAGACATGGTGCGCTCCATGCTGTCGGAATCATTGCGGGCGGTGATTTCTCAGAA
CCTGCTTAAAAAGGTGAATGGCGGCCGGGTAGCAGCCCATGAAATTATGCTGGGCACGCCGGCCATTCGTAACCTGATCC
GTGAAGACAAGGTCGCCCAGATGTATTCCGTGATTCAAACCGGCATGGCCCATGGCATGCAGACCATGGATCAGTCGCTC
AAGCGGCTGGTGGCCAGCGGTCAGGTGGCCCCTGCCGATGCCCGCCTGCGTGCGGCAGATCCGCAAAGCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

77.812

95.64

0.744
  pilT Vibrio cholerae strain A1552

77.812

95.64

0.744
  pilT Acinetobacter baumannii D1279779

74.419

100

0.744
  pilT Acinetobacter baumannii strain A118

74.419

100

0.744
  pilT Acinetobacter nosocomialis M2

74.128

100

0.741
  pilT Acinetobacter baylyi ADP1

73.547

100

0.735
  pilT Pseudomonas stutzeri DSM 10701

73.256

100

0.733
  pilT Pseudomonas aeruginosa PAK

72.674

100

0.727
  pilT Legionella pneumophila strain Lp02

74.383

94.186

0.701
  pilT Legionella pneumophila strain ERS1305867

74.383

94.186

0.701
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.729

99.709

0.506
  pilU Pseudomonas stutzeri DSM 10701

41.298

98.547

0.407
  pilU Vibrio cholerae strain A1552

40.762

99.128

0.404
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment