Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB9R84_RS12135 Genome accession   NZ_CP166304
Coordinates   2530303..2531412 (-) Length   369 a.a.
NCBI ID   WP_317074629.1    Uniprot ID   -
Organism   Oceanimonas smirnovii strain OS2020-133     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2525303..2536412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9R84_RS12115 (AB9R84_12105) brnQ 2525843..2527147 (-) 1305 WP_418139399.1 branched-chain amino acid transport system II carrier protein -
  AB9R84_RS12120 (AB9R84_12110) - 2527312..2528019 (-) 708 WP_418139400.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  AB9R84_RS12125 (AB9R84_12115) srmB 2528090..2529391 (+) 1302 WP_418139401.1 ATP-dependent RNA helicase SrmB -
  AB9R84_RS12130 (AB9R84_12120) yaaA 2529516..2530295 (-) 780 WP_395535141.1 peroxide stress protein YaaA -
  AB9R84_RS12135 (AB9R84_12125) pilU 2530303..2531412 (-) 1110 WP_317074629.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB9R84_RS12140 (AB9R84_12130) pilT 2531429..2532463 (-) 1035 WP_317074630.1 type IV pilus twitching motility protein PilT Machinery gene
  AB9R84_RS12145 (AB9R84_12135) - 2532504..2533196 (+) 693 WP_317074631.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB9R84_RS12150 (AB9R84_12140) proC 2533207..2534031 (+) 825 WP_395535140.1 pyrroline-5-carboxylate reductase -
  AB9R84_RS12155 (AB9R84_12145) - 2534041..2534592 (+) 552 WP_395535139.1 YggT family protein -
  AB9R84_RS12160 (AB9R84_12150) - 2534661..2535074 (+) 414 WP_395535138.1 DUF4426 domain-containing protein -
  AB9R84_RS12165 (AB9R84_12155) rdgB 2535085..2535690 (+) 606 WP_019933100.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41068.83 Da        Isoelectric Point: 5.8398

>NTDB_id=1034145 AB9R84_RS12135 WP_317074629.1 2530303..2531412(-) (pilU) [Oceanimonas smirnovii strain OS2020-133]
MELASLLSRMCEHKASDMYISVGIAPTIKTQGKLMPLGEQPLTEQETLALVESCMTAENKALFHQQKEANFAIHDDDHGR
FRVSAFWQQDKAGMVLRRIESRIPSFEELYLPETLRDIAMAKRGLVLFVGGTGTGKSTTQAAMIGHRNGHADGHILTIED
PVEFVHQHQRSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSEETMGYALSFAETGHLCMATLHANNANQAIDRI
MHLVPENKHRQLLFDLSFNLKAIVAQQLIPTQDGTQRRAAFEILLNTPLIADVIRQGDMHKLKEIMAKSRESGMQTFDQA
LFDLYSQQHIGYTEALAYADSPNDLRLMIKLQGGGGLDSGLFDNVTIES

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1034145 AB9R84_RS12135 WP_317074629.1 2530303..2531412(-) (pilU) [Oceanimonas smirnovii strain OS2020-133]
ATGGAACTGGCGTCATTATTAAGCCGCATGTGCGAGCACAAGGCCTCGGACATGTATATTTCGGTGGGCATTGCGCCCAC
CATCAAAACCCAGGGCAAACTCATGCCTCTGGGCGAGCAGCCACTGACTGAGCAGGAAACGCTGGCGCTGGTTGAATCCT
GCATGACGGCGGAAAACAAGGCGCTTTTTCATCAGCAAAAAGAAGCCAACTTTGCCATACACGACGATGATCATGGCCGC
TTTCGGGTCAGCGCTTTCTGGCAGCAAGACAAGGCCGGCATGGTGCTGCGGCGCATTGAAAGCCGGATTCCGTCTTTTGA
GGAGCTATATCTGCCCGAAACCCTGCGCGACATTGCCATGGCCAAGCGCGGTCTGGTGCTGTTTGTGGGCGGCACCGGCA
CCGGTAAGTCCACCACTCAGGCGGCCATGATAGGCCACCGCAACGGCCATGCCGACGGCCATATTCTGACCATTGAAGAT
CCGGTGGAATTTGTCCATCAGCATCAACGTTCCATTATTACTCAGCGGGAAGTGGGTATCGACACCGAGTCGTTCGATGC
TGCGCTGAAAAGCTCGTTGCGTCAGGCCCCGGATGTCATCTTGATTGGTGAAATCCGCTCGGAAGAAACCATGGGTTATG
CCCTGTCGTTTGCTGAAACCGGGCATTTGTGCATGGCTACCCTGCATGCCAACAACGCCAACCAGGCCATTGACCGAATT
ATGCACCTGGTGCCGGAAAACAAGCATCGCCAGCTGCTGTTTGATTTGTCGTTCAACCTGAAAGCCATAGTGGCTCAGCA
ATTGATCCCCACTCAGGATGGCACTCAGCGCCGGGCCGCGTTTGAGATTTTGCTGAACACGCCGCTGATTGCCGATGTGA
TCCGTCAGGGAGATATGCACAAGCTCAAGGAGATTATGGCCAAATCCCGTGAGTCCGGTATGCAAACCTTTGATCAGGCT
CTGTTTGACCTCTACAGTCAGCAGCATATAGGCTACACCGAAGCACTGGCCTATGCTGACTCTCCCAACGATCTGCGGCT
GATGATCAAGCTGCAGGGCGGCGGCGGGCTCGACTCCGGCCTGTTCGACAATGTAACCATCGAATCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

58.967

99.729

0.588
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

58.523

95.393

0.558
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.899

93.496

0.401
  pilT Neisseria gonorrhoeae MS11

40.979

88.618

0.363
  pilT Neisseria meningitidis 8013

40.979

88.618

0.363
  pilT Acinetobacter baylyi ADP1

39.181

92.683

0.363
  pilT Legionella pneumophila strain Lp02

40.181

89.702

0.36
  pilT Legionella pneumophila strain ERS1305867

40.181

89.702

0.36

Multiple sequence alignment