Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB8599_RS13315 Genome accession   NZ_CP166194
Coordinates   2997821..2998858 (-) Length   345 a.a.
NCBI ID   WP_407331380.1    Uniprot ID   -
Organism   Enterovibrio sp. 27052020O     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2992821..3003858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8599_RS13285 (AB8599_13290) rsmE 2992978..2993709 (+) 732 WP_407331374.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB8599_RS13290 (AB8599_13295) gshB 2993723..2994670 (+) 948 WP_407331375.1 glutathione synthase -
  AB8599_RS13295 (AB8599_13300) - 2994784..2995347 (+) 564 WP_407331376.1 YqgE/AlgH family protein -
  AB8599_RS13300 (AB8599_13305) ruvX 2995358..2995789 (+) 432 WP_407331377.1 Holliday junction resolvase RuvX -
  AB8599_RS13305 (AB8599_13310) - 2995825..2996580 (-) 756 WP_407331378.1 EAL domain-containing protein -
  AB8599_RS13310 (AB8599_13315) pilU 2996697..2997818 (-) 1122 WP_407331379.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8599_RS13315 (AB8599_13320) pilT 2997821..2998858 (-) 1038 WP_407331380.1 type IV pilus twitching motility protein PilT Machinery gene
  AB8599_RS13320 (AB8599_13325) - 2998885..2999592 (+) 708 WP_407331381.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB8599_RS13325 (AB8599_13330) proC 2999661..3000479 (+) 819 WP_407331382.1 pyrroline-5-carboxylate reductase -
  AB8599_RS13330 (AB8599_13335) - 3000489..3001058 (+) 570 WP_407331383.1 YggT family protein -
  AB8599_RS13335 (AB8599_13340) yggU 3001062..3001373 (+) 312 WP_407331384.1 DUF167 family protein YggU -
  AB8599_RS13340 (AB8599_13345) - 3001438..3001875 (+) 438 WP_407331385.1 DUF4426 domain-containing protein -
  AB8599_RS13345 (AB8599_13350) - 3001997..3002596 (+) 600 WP_407331386.1 XTP/dITP diphosphatase -
  AB8599_RS13350 (AB8599_13355) hemW 3002603..3003754 (+) 1152 WP_407331387.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38181.56 Da        Isoelectric Point: 6.4414

>NTDB_id=1033641 AB8599_RS13315 WP_407331380.1 2997821..2998858(-) (pilT) [Enterovibrio sp. 27052020O]
MDITELLDFSVKHNASDLHLSAGVPPMVRVDGDVRKLSLPALEHNDVHRLIFDIMNDAQQREFEQNLEVDFSFELASVGR
FRVNAFHQSRGCAAVFRNIPSKIPTLEAINAPSVFEQFVNYQKGLVLVTGPTGSGKSTTLAAMVDKINREQHRHVLTIED
PIEFVHEQKNCLVNQREVHRDTHSFNNALRSALREDPDVILVGELRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGADKPMVRSMLSESLRAVVSQKLIKQVTGGRVAAHEVMIATPAIRNLIREDKVAQMYSMIQTGSGMGMQTMEQSL
RNLMMQGVIDSNEANKALDSSGTGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1033641 AB8599_RS13315 WP_407331380.1 2997821..2998858(-) (pilT) [Enterovibrio sp. 27052020O]
ATGGATATTACTGAATTATTGGACTTTAGTGTAAAACATAATGCCTCAGATCTACATCTTTCTGCGGGTGTTCCACCAAT
GGTCCGCGTTGATGGTGATGTCCGAAAGCTTAGCTTACCTGCCCTTGAACACAATGATGTTCACCGCCTCATTTTCGATA
TTATGAACGACGCTCAACAGCGAGAGTTTGAGCAAAACCTTGAAGTGGATTTCTCGTTTGAACTGGCATCTGTTGGCCGT
TTTCGTGTTAATGCATTTCACCAATCACGCGGCTGCGCAGCGGTATTTCGAAATATTCCCTCAAAGATCCCAACGCTAGA
AGCGATCAATGCACCTTCGGTTTTTGAGCAGTTTGTTAATTATCAAAAAGGCTTAGTGCTGGTCACGGGGCCTACAGGTT
CCGGTAAATCCACCACGTTGGCTGCGATGGTGGACAAGATTAACCGCGAGCAACACCGCCATGTGCTGACGATTGAAGAC
CCGATTGAATTTGTTCATGAGCAGAAAAACTGCTTGGTTAACCAGCGTGAAGTTCACCGTGATACGCACTCATTTAATAA
CGCCCTTCGTTCGGCACTGCGTGAAGACCCTGATGTTATTTTGGTGGGTGAGTTGCGTGATAAAGAAACCATTAGCCTTG
CGCTAACTGCCGCTGAAACGGGTCATTTGGTGTTTGGCACCTTGCACACCTCCAGTGCTGCGAAAACCATTGACCGGATT
ATTGATGTGTTCCCCGGTGCCGATAAGCCGATGGTGCGCTCTATGTTGTCTGAATCGCTACGCGCCGTGGTGTCACAAAA
ACTGATTAAGCAGGTTACTGGCGGGCGAGTGGCAGCCCATGAAGTGATGATTGCAACACCTGCGATTCGAAACCTGATTC
GTGAAGACAAAGTGGCGCAGATGTATTCGATGATCCAAACCGGCTCAGGCATGGGCATGCAAACCATGGAGCAATCACTG
CGCAACCTGATGATGCAGGGTGTGATTGATAGCAATGAAGCCAACAAAGCATTAGATAGTTCAGGGACAGGTTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

76.991

98.261

0.757
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.991

98.261

0.757
  pilT Acinetobacter baumannii strain A118

70.03

97.681

0.684
  pilT Acinetobacter nosocomialis M2

70.03

97.681

0.684
  pilT Acinetobacter baumannii D1279779

70.03

97.681

0.684
  pilT Pseudomonas aeruginosa PAK

69.643

97.391

0.678
  pilT Pseudomonas stutzeri DSM 10701

69.345

97.391

0.675
  pilT Acinetobacter baylyi ADP1

69.394

95.652

0.664
  pilT Legionella pneumophila strain Lp02

67.359

97.681

0.658
  pilT Legionella pneumophila strain ERS1305867

67.359

97.681

0.658
  pilT Neisseria gonorrhoeae MS11

62.281

99.13

0.617
  pilT Neisseria meningitidis 8013

64.242

95.652

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.267

98.841

0.487
  pilU Pseudomonas stutzeri DSM 10701

41.813

99.13

0.414
  pilU Vibrio cholerae strain A1552

41.52

99.13

0.412
  pilU Acinetobacter baylyi ADP1

39.474

99.13

0.391

Multiple sequence alignment