Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB8599_RS13310 Genome accession   NZ_CP166194
Coordinates   2996697..2997818 (-) Length   373 a.a.
NCBI ID   WP_407331379.1    Uniprot ID   -
Organism   Enterovibrio sp. 27052020O     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2991697..3002818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8599_RS13280 (AB8599_13285) endA 2992165..2992860 (+) 696 WP_407331373.1 deoxyribonuclease I -
  AB8599_RS13285 (AB8599_13290) rsmE 2992978..2993709 (+) 732 WP_407331374.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB8599_RS13290 (AB8599_13295) gshB 2993723..2994670 (+) 948 WP_407331375.1 glutathione synthase -
  AB8599_RS13295 (AB8599_13300) - 2994784..2995347 (+) 564 WP_407331376.1 YqgE/AlgH family protein -
  AB8599_RS13300 (AB8599_13305) ruvX 2995358..2995789 (+) 432 WP_407331377.1 Holliday junction resolvase RuvX -
  AB8599_RS13305 (AB8599_13310) - 2995825..2996580 (-) 756 WP_407331378.1 EAL domain-containing protein -
  AB8599_RS13310 (AB8599_13315) pilU 2996697..2997818 (-) 1122 WP_407331379.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8599_RS13315 (AB8599_13320) pilT 2997821..2998858 (-) 1038 WP_407331380.1 type IV pilus twitching motility protein PilT Machinery gene
  AB8599_RS13320 (AB8599_13325) - 2998885..2999592 (+) 708 WP_407331381.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB8599_RS13325 (AB8599_13330) proC 2999661..3000479 (+) 819 WP_407331382.1 pyrroline-5-carboxylate reductase -
  AB8599_RS13330 (AB8599_13335) - 3000489..3001058 (+) 570 WP_407331383.1 YggT family protein -
  AB8599_RS13335 (AB8599_13340) yggU 3001062..3001373 (+) 312 WP_407331384.1 DUF167 family protein YggU -
  AB8599_RS13340 (AB8599_13345) - 3001438..3001875 (+) 438 WP_407331385.1 DUF4426 domain-containing protein -
  AB8599_RS13345 (AB8599_13350) - 3001997..3002596 (+) 600 WP_407331386.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41495.75 Da        Isoelectric Point: 6.3879

>NTDB_id=1033640 AB8599_RS13310 WP_407331379.1 2996697..2997818(-) (pilU) [Enterovibrio sp. 27052020O]
MADQTPLFQLLDAMLTQRASDCYITVGAPCMLRVDGSLTPSGETLDVEGVKALISKAMDTDTHKKFMEEREANFALVTES
GRFRVSAFWQRDLPGMVIRRIETKIPEIQTLGLPDVIQDLSIAKRGLVLIVGATGSGKSTSMAAMLGFRNTHRSGHILTV
EDPIEFIHPHKKCIVTQREIGIDTESYEVALKNSLRQAPDMIMIGEIRTRETMEYAMQFAETGHLCIATLHANNANQALE
RVLHLVPKERREQFLFDLSLNLRGVLAQQLVPDISGVGRHGAYELLLNTSHVSDLIRRGELHELKASISKGENAGMCTFD
QSLFALFKDGKISQDEALHHADSRNELKLMMKMAEQSSSVLSLADVTIEKMHN

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1033640 AB8599_RS13310 WP_407331379.1 2996697..2997818(-) (pilU) [Enterovibrio sp. 27052020O]
ATGGCGGACCAAACACCACTTTTTCAATTGCTTGACGCCATGCTCACTCAACGTGCATCAGATTGCTATATCACCGTGGG
TGCGCCTTGTATGCTGCGTGTTGATGGCAGCTTAACGCCCAGCGGTGAGACGTTAGATGTTGAAGGTGTGAAGGCCTTGA
TATCAAAAGCCATGGATACTGATACGCATAAAAAGTTCATGGAAGAACGCGAAGCCAATTTTGCTTTGGTCACCGAGTCG
GGCCGTTTTCGTGTCAGTGCATTCTGGCAGCGAGATTTGCCAGGCATGGTGATTCGACGTATTGAAACGAAGATCCCAGA
GATCCAAACGCTGGGTTTACCGGATGTGATTCAAGATCTCTCGATTGCGAAGCGCGGGTTAGTGTTGATAGTGGGCGCCA
CAGGGTCGGGGAAATCGACGTCGATGGCGGCCATGCTAGGGTTTCGAAATACACATCGCAGTGGTCATATTCTGACGGTG
GAAGATCCGATAGAGTTTATCCATCCTCATAAAAAGTGCATCGTGACCCAGCGCGAGATAGGCATTGATACTGAAAGCTA
TGAAGTGGCATTGAAAAACTCGCTACGTCAGGCGCCAGACATGATTATGATTGGTGAAATTAGAACGCGAGAGACCATGG
AATATGCCATGCAGTTTGCGGAGACAGGTCATCTGTGTATCGCCACCCTTCATGCCAACAATGCCAATCAGGCACTTGAG
CGCGTGTTGCACTTGGTGCCGAAGGAGCGCAGAGAGCAGTTCTTATTTGATTTGTCGCTTAACCTACGTGGTGTCTTAGC
TCAGCAGTTGGTGCCCGACATTAGCGGTGTAGGTCGTCATGGTGCTTACGAATTATTGCTTAATACTTCACACGTGTCAG
ATCTGATCCGCCGTGGCGAATTGCATGAGCTTAAAGCCAGTATCAGCAAAGGCGAAAATGCAGGGATGTGTACATTTGAC
CAATCTTTATTTGCTTTGTTTAAAGACGGTAAGATTTCTCAGGACGAAGCACTGCATCATGCAGATTCTCGTAATGAATT
GAAGTTGATGATGAAAATGGCAGAGCAGTCTTCCAGTGTACTATCGCTTGCCGATGTGACCATCGAGAAAATGCACAACT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

70.231

92.761

0.651
  pilU Pseudomonas stutzeri DSM 10701

54.986

94.102

0.517
  pilU Acinetobacter baylyi ADP1

52.113

95.174

0.496
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.741

94.102

0.383
  pilT Pseudomonas aeruginosa PAK

40.413

90.885

0.367
  pilT Pseudomonas stutzeri DSM 10701

40.299

89.812

0.362

Multiple sequence alignment