Detailed information NTG1033620

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: AB8K37_RS22650
• Genome accession: NZ_CP166186
• Coordinates: 4660129..4661109 (+)
• Length: 326 a.a.
• NCBI ID: WP_023149798.1
• Uniprot ID: A0A1X9TJB3
• Organism: Escherichia coli strain CRE78
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4655129..4666109

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB8K37_RS22620 (AB8K37_22585)	yggM	4655734..4656681 (+)	948	WP_000745231.1	DUF1202 family protein	-
AB8K37_RS22625 (AB8K37_22590)	hemW	4656806..4657942 (-)	1137	WP_000239953.1	radical SAM family heme chaperone HemW	-
AB8K37_RS22630 (AB8K37_22595)	rdgB	4657935..4658528 (-)	594	WP_001174738.1	XTP/dITP diphosphatase	-
AB8K37_RS22635 (AB8K37_22600)	yggU	4658536..4658826 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
AB8K37_RS22640 (AB8K37_22605)	yggT	4658823..4659389 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
AB8K37_RS22645 (AB8K37_22610)	yggS	4659407..4660111 (-)	705	WP_000997804.1	pyridoxal phosphate homeostasis protein	-
AB8K37_RS22650 (AB8K37_22615)	pilT	4660129..4661109 (+)	981	WP_023149798.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB8K37_RS22655 (AB8K37_22620)	ruvX	4661283..4661699 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
AB8K37_RS22660 (AB8K37_22625)	yqgE	4661699..4662262 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
AB8K37_RS22665 (AB8K37_22630)	gshB	4662371..4663321 (-)	951	WP_000593273.1	glutathione synthase	-
AB8K37_RS22670 (AB8K37_22635)	rsmE	4663334..4664065 (-)	732	WP_001300912.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
AB8K37_RS22675 (AB8K37_22640)	endA	4664145..4664852 (-)	708	WP_023149797.1	deoxyribonuclease I	-
AB8K37_RS22680 (AB8K37_22645)	yggI	4664947..4665444 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 36001.19 Da
• Isoelectric Point: 5.9975

>NTDB_id=1033620 AB8K37_RS22650 WP_023149798.1 4660129..4661109(+) (pilT) [Escherichia coli strain CRE78]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAGLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=1033620 AB8K37_RS22650 WP_023149798.1 4660129..4661109(+) (pilT) [Escherichia coli strain CRE78]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAACAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGGGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

3D structure

• AlphaFold DB: A0A1X9TJB3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.235	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.235	100	0.494
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.177	100	0.463
pilT	Acinetobacter nosocomialis M2	46.177	100	0.463
pilT	Acinetobacter baumannii strain A118	46.177	100	0.463
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	40.379	97.239	0.393
pilU	Pseudomonas stutzeri DSM 10701	37.576	100	0.38
pilB	Legionella pneumophila strain ERS1305867	31.481	100	0.365
pilU	Acinetobacter baylyi ADP1	36.646	98.773	0.362