Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB8E34_RS12675 Genome accession   NZ_CP165964
Coordinates   2789615..2790649 (+) Length   344 a.a.
NCBI ID   WP_318644021.1    Uniprot ID   -
Organism   Pseudomonas benzenivorans strain jeju3-2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2784615..2795649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8E34_RS12655 (AB8E34_12615) - 2785229..2787211 (-) 1983 WP_369958562.1 dynamin family protein -
  AB8E34_RS12660 (AB8E34_12620) - 2787415..2788005 (-) 591 WP_318644024.1 YggT family protein -
  AB8E34_RS12665 (AB8E34_12625) proC 2788016..2788834 (-) 819 WP_318644023.1 pyrroline-5-carboxylate reductase -
  AB8E34_RS12670 (AB8E34_12630) - 2788849..2789541 (-) 693 WP_369958563.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB8E34_RS12675 (AB8E34_12635) pilT 2789615..2790649 (+) 1035 WP_318644021.1 type IV pilus twitching motility protein PilT Machinery gene
  AB8E34_RS12680 (AB8E34_12640) pilU 2790761..2791906 (+) 1146 WP_318644020.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8E34_RS12685 (AB8E34_12645) - 2792011..2792412 (+) 402 WP_318644019.1 TM2 domain-containing protein -
  AB8E34_RS12690 (AB8E34_12650) - 2792471..2793742 (-) 1272 WP_318644018.1 dihydroorotase -
  AB8E34_RS12695 (AB8E34_12655) - 2793742..2794746 (-) 1005 WP_318644017.1 aspartate carbamoyltransferase catalytic subunit -
  AB8E34_RS12700 (AB8E34_12660) pyrR 2794758..2795264 (-) 507 WP_318644016.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37901.64 Da        Isoelectric Point: 6.8361

>NTDB_id=1032596 AB8E34_RS12675 WP_318644021.1 2789615..2790649(+) (pilT) [Pseudomonas benzenivorans strain jeju3-2]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALMYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDACL
KGLVAKGLVSRESAREKSKNPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1032596 AB8E34_RS12675 WP_318644021.1 2789615..2790649(+) (pilT) [Pseudomonas benzenivorans strain jeju3-2]
ATGGATATCACCGAGCTGCTTGCCTTCAGCGCCAAGCAAGGCGCGTCGGACCTGCATCTCTCTGCCGGTCTGCCGCCGAT
GATTCGGGTCGACGGCGATGTGCGTCGGATCAACCTGCCGGCCCTGGATCACAAGCAGGTGCACGCGCTGATGTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAAGAATTCCTGGAAACCGACTTCTCCTTCGAAGTGCCCGGGGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGGACCATTCCGTCCAAGGTGCTGACCATGGA
AGACCTCGGCATGGGCGAGGTGTTCCGCAAGATCACCGACGTGCCGCGCGGCCTGGTGCTGGTCACCGGGCCCACGGGTT
CGGGCAAGTCGACCACCCTGGCGGCCATGCTCGACTACCTCAACAGCACCAAGTACCACCATATCCTGACCATCGAGGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGTCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCTGGGCTTCTCCGA
GGCCCTGCGCTCGGCGCTGCGTGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCCGAGGAGAAGTCCATGGTTCGTTCCATGCTCTCGGAGTCCTTGCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCTATCCAGACCGGCGGCTCGCTGGGCATGCAGACCCTCGACGCCTGCCTC
AAGGGCCTGGTCGCCAAGGGCCTGGTCAGTCGCGAGAGTGCCCGGGAGAAATCCAAGAACCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

96.221

100

0.962
  pilT Pseudomonas aeruginosa PAK

95.058

100

0.951
  pilT Acinetobacter baumannii D1279779

84.593

100

0.846
  pilT Acinetobacter baumannii strain A118

84.593

100

0.846
  pilT Acinetobacter nosocomialis M2

84.302

100

0.843
  pilT Acinetobacter baylyi ADP1

81.977

100

0.82
  pilT Legionella pneumophila strain Lp02

73.547

100

0.735
  pilT Legionella pneumophila strain ERS1305867

73.547

100

0.735
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Vibrio cholerae strain A1552

41.493

97.384

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39

Multiple sequence alignment