Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB8E34_RS12680 Genome accession   NZ_CP165964
Coordinates   2790761..2791906 (+) Length   381 a.a.
NCBI ID   WP_318644020.1    Uniprot ID   -
Organism   Pseudomonas benzenivorans strain jeju3-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2785761..2796906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8E34_RS12660 (AB8E34_12620) - 2787415..2788005 (-) 591 WP_318644024.1 YggT family protein -
  AB8E34_RS12665 (AB8E34_12625) proC 2788016..2788834 (-) 819 WP_318644023.1 pyrroline-5-carboxylate reductase -
  AB8E34_RS12670 (AB8E34_12630) - 2788849..2789541 (-) 693 WP_369958563.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB8E34_RS12675 (AB8E34_12635) pilT 2789615..2790649 (+) 1035 WP_318644021.1 type IV pilus twitching motility protein PilT Machinery gene
  AB8E34_RS12680 (AB8E34_12640) pilU 2790761..2791906 (+) 1146 WP_318644020.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8E34_RS12685 (AB8E34_12645) - 2792011..2792412 (+) 402 WP_318644019.1 TM2 domain-containing protein -
  AB8E34_RS12690 (AB8E34_12650) - 2792471..2793742 (-) 1272 WP_318644018.1 dihydroorotase -
  AB8E34_RS12695 (AB8E34_12655) - 2793742..2794746 (-) 1005 WP_318644017.1 aspartate carbamoyltransferase catalytic subunit -
  AB8E34_RS12700 (AB8E34_12660) pyrR 2794758..2795264 (-) 507 WP_318644016.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  AB8E34_RS12705 (AB8E34_12665) ruvX 2795294..2795737 (-) 444 WP_318644015.1 Holliday junction resolvase RuvX -
  AB8E34_RS12710 (AB8E34_12670) - 2795737..2796306 (-) 570 WP_318644014.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42491.66 Da        Isoelectric Point: 6.5932

>NTDB_id=1032597 AB8E34_RS12680 WP_318644020.1 2790761..2791906(+) (pilU) [Pseudomonas benzenivorans strain jeju3-2]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNPMSPEQTRETVYAVMNEQQRREFAENHECNFAISARGVGR
FRVSAFYQRNLAGMVLRRIETNIPTLDDLKLPEVLKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQNCIVTQREVGIDTESYEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSTEQGMQTFDQA
LYALYSQGEITYEDALLYADSANDLRLMIKLGSESDGDHLAQLSEGLSLETTPEDPGRRFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1032597 AB8E34_RS12680 WP_318644020.1 2790761..2791906(+) (pilU) [Pseudomonas benzenivorans strain jeju3-2]
ATGGAATTCGAAAAACTGCTGCGCCTGATGGTGGAGAAGGGCGGCTCGGACCTGTTCATCACCGCCGGGGTGCCACCGTC
CATGAAGGTCAACGGCAAGATCATGCCGGTCACCAAGAACCCGATGTCGCCGGAGCAGACCCGGGAAACCGTGTACGCGG
TGATGAACGAGCAGCAGCGCCGGGAGTTCGCCGAGAATCACGAATGCAACTTCGCCATCAGCGCCCGCGGCGTGGGGCGT
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTGGCGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCGACCCTGGA
TGACCTCAAGCTGCCGGAGGTGCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGCTGTTCGTCGGCGCCACCGGCA
CCGGCAAGTCGACTTCCCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAATACATCCACCAGCACCAGAACTGCATCGTCACCCAGCGCGAGGTCGGGATCGACACCGAGTCCTACGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCCCCGGACGTGATCCTGATCGGCGAGGTGCGCACCCGCGAGACCATGGATCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCCGCCGACCGGCAGAACCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGATGGCAAGGGGCGGCGGGCGGTGATCGAGGTGTTGATCAACACCCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGGGTCTGATGAAGCGCTCCACCGAACAGGGCATGCAGACCTTCGACCAGGCG
CTCTATGCCCTCTACAGCCAGGGCGAGATCACCTACGAGGATGCGCTGCTGTACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGCTCGGAAAGCGACGGCGATCACCTGGCCCAGCTGAGCGAGGGCCTGTCCCTGGAGACCACACCGG
AAGACCCGGGGCGGCGTTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

91.601

100

0.916
  pilU Acinetobacter baylyi ADP1

63.866

93.701

0.598
  pilU Vibrio cholerae strain A1552

59.429

91.864

0.546
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.155

88.189

0.381

Multiple sequence alignment