Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB8E26_RS02780 Genome accession   NZ_CP165963
Coordinates   592515..593552 (-) Length   345 a.a.
NCBI ID   WP_070208322.1    Uniprot ID   A0A1E7RM02
Organism   Stenotrophomonas rhizophila strain busan1-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 587515..598552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8E26_RS02750 (AB8E26_02760) - 587698..587916 (-) 219 WP_159495949.1 DUF4287 domain-containing protein -
  AB8E26_RS02755 (AB8E26_02765) - 588088..588903 (+) 816 Protein_538 alpha/beta fold hydrolase -
  AB8E26_RS02760 (AB8E26_02770) - 589019..589731 (-) 713 Protein_539 aldo/keto reductase -
  AB8E26_RS02765 (AB8E26_02775) - 589867..590529 (-) 663 WP_369969416.1 PIN domain-containing protein -
  AB8E26_RS02770 (AB8E26_02780) - 590537..591115 (-) 579 WP_313423747.1 DUF4234 domain-containing protein -
  AB8E26_RS02775 (AB8E26_02785) pilU 591294..592436 (-) 1143 WP_038690016.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8E26_RS02780 (AB8E26_02790) pilT 592515..593552 (-) 1038 WP_070208322.1 type IV pilus twitching motility protein PilT Machinery gene
  AB8E26_RS02785 (AB8E26_02795) - 593639..594319 (+) 681 WP_369969417.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB8E26_RS02790 (AB8E26_02800) proC 594351..595172 (+) 822 WP_369969418.1 pyrroline-5-carboxylate reductase -
  AB8E26_RS02795 (AB8E26_02805) - 595250..596131 (-) 882 WP_159495943.1 LysR substrate-binding domain-containing protein -
  AB8E26_RS02800 (AB8E26_02810) - 596229..596831 (+) 603 WP_369969419.1 short chain dehydrogenase -
  AB8E26_RS02805 (AB8E26_02815) soxR 596964..597398 (-) 435 WP_181393942.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38569.36 Da        Isoelectric Point: 6.5797

>NTDB_id=1032567 AB8E26_RS02780 WP_070208322.1 592515..593552(-) (pilT) [Stenotrophomonas rhizophila strain busan1-1]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLAVPPIFRELIDQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQMGMMTLDQHL
QDLVKRSLITRNQAKEYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1032567 AB8E26_RS02780 WP_070208322.1 592515..593552(-) (pilT) [Stenotrophomonas rhizophila strain busan1-1]
ATGGATATCGCCGAACTGTTGGCGTTTTCCGTAAAGAACAAAGCGTCGGATCTTCATCTGTCGGCAGGCCTGCCGCCGAT
GATCCGCGTTGACGGCGATGTCCGTCGCATCAACATCCCGGCCTTGGACCACAAGCAGGTGCATGCGCTTGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTCGACTTCTCGTTCGAGATTCCGTCGCTTGCGCGC
TTCCGCGTCAATGCGTTCAATCAGAACCGGGGCGCCGGTGCGGTGTTCCGTACCATTCCCTCGGAAGTGTTGACGTTGGA
AGACCTGGCCGTGCCGCCGATCTTCCGCGAACTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGTGACACGCACGGCTTCAACGA
AGCGCTGCGCTCGGCCCTGCGTGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCCGAAACCGGTCACCTGGTGTTCGGCACGCTGCATACCAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGTGCGGTGATCTCCCAGGC
CCTGCTGAAGAAGGTCGGTGGCGGGCGTACCGCCGCGTGGGAAATCATGGTGGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCCAGCAGATGGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGGAACCAGGCCAAGGAATACGCCAAGGACAAGCGCATCTTCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E7RM02

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.779

99.71

0.786
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

78.466

98.261

0.771
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.396

98.841

0.646
  pilT Neisseria gonorrhoeae MS11

65.103

98.841

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Acinetobacter baylyi ADP1

40.588

98.551

0.4
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Vibrio cholerae strain A1552

40.299

97.101

0.391

Multiple sequence alignment