Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB8E26_RS02775 Genome accession   NZ_CP165963
Coordinates   591294..592436 (-) Length   380 a.a.
NCBI ID   WP_038690016.1    Uniprot ID   A0A2R7QWK9
Organism   Stenotrophomonas rhizophila strain busan1-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 586294..597436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8E26_RS02745 (AB8E26_02755) - 586932..587633 (-) 702 WP_369969415.1 hypothetical protein -
  AB8E26_RS02750 (AB8E26_02760) - 587698..587916 (-) 219 WP_159495949.1 DUF4287 domain-containing protein -
  AB8E26_RS02755 (AB8E26_02765) - 588088..588903 (+) 816 Protein_538 alpha/beta fold hydrolase -
  AB8E26_RS02760 (AB8E26_02770) - 589019..589731 (-) 713 Protein_539 aldo/keto reductase -
  AB8E26_RS02765 (AB8E26_02775) - 589867..590529 (-) 663 WP_369969416.1 PIN domain-containing protein -
  AB8E26_RS02770 (AB8E26_02780) - 590537..591115 (-) 579 WP_313423747.1 DUF4234 domain-containing protein -
  AB8E26_RS02775 (AB8E26_02785) pilU 591294..592436 (-) 1143 WP_038690016.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8E26_RS02780 (AB8E26_02790) pilT 592515..593552 (-) 1038 WP_070208322.1 type IV pilus twitching motility protein PilT Machinery gene
  AB8E26_RS02785 (AB8E26_02795) - 593639..594319 (+) 681 WP_369969417.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB8E26_RS02790 (AB8E26_02800) proC 594351..595172 (+) 822 WP_369969418.1 pyrroline-5-carboxylate reductase -
  AB8E26_RS02795 (AB8E26_02805) - 595250..596131 (-) 882 WP_159495943.1 LysR substrate-binding domain-containing protein -
  AB8E26_RS02800 (AB8E26_02810) - 596229..596831 (+) 603 WP_369969419.1 short chain dehydrogenase -
  AB8E26_RS02805 (AB8E26_02815) soxR 596964..597398 (-) 435 WP_181393942.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42394.76 Da        Isoelectric Point: 6.8287

>NTDB_id=1032566 AB8E26_RS02775 WP_038690016.1 591294..592436(-) (pilU) [Stenotrophomonas rhizophila strain busan1-1]
MNTTTTTIDFTSFLKLMAHQKASDLFITAGMPPAIKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAI
GLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNHNSTG
HIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNA
NQAMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPSPDGKSRKVAMEIMLGTPLVQDYIRDGEIHKLKEVMKESVQLG
MRTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=1032566 AB8E26_RS02775 WP_038690016.1 591294..592436(-) (pilU) [Stenotrophomonas rhizophila strain busan1-1]
ATGAACACCACCACCACGACCATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCAT
CACCGCGGGCATGCCGCCGGCGATCAAGGTCAACGGCAAGATCTCGCCGATTACCCAGACCCCGCTCACGCCGCAGCAGA
GCCGCGACCTGGTGCTGAACGTGATGACGCCGGCGCAGCGCGAAGAGTTCGAAAAGACCCACGAGTGCAACTTCGCCATC
GGCCTGTCCGGCGTCGGCCGGTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTAGGCATGGTGCTGCGTCGCATCGA
GACGCGCATTCCCACCGTGGAAGAGCTGAGTCTGCCGCCGATCATCAAGACGCTGGCAATGACCAAGCGCGGCATCATTC
TGTTCGTGGGTGCTACCGGTACCGGTAAATCGACTTCGCTGGCGGCGATGATCGGCTACCGCAATCACAACTCGACCGGG
CACATCATCACCATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCAGCGCTGAAGAACACCCTGCGCCAAGCGCCGGACGTGATCATGATCGGCGAGGTGCGCA
CCCGCGAGGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCC
AACCAGGCGATGGACCGCATCATCAACTTCTTCCCCGAAGACCGCCGCAACCAGCTGCTGATGGATCTGTCGCTGAACCT
CAAGGGCGTGGTGGCGCAGCAGTTGATTCCCTCGCCGGATGGCAAGTCGCGCAAGGTGGCGATGGAAATCATGCTGGGCA
CGCCGCTGGTGCAGGATTACATCCGCGATGGCGAGATCCACAAGCTCAAGGAAGTAATGAAGGAGTCGGTGCAGCTGGGC
ATGCGCACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAGATCAGCTACGAAGACGCACTGCGCTACGCCGA
TTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTGTCGCAGGGCGGCGACGCACGCACGCTGTCGCAGGGCCTGGACGGGG
TGGAGATCTCCGAAGTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R7QWK9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

96.579

0.653
  pilU Acinetobacter baylyi ADP1

63.866

93.947

0.6
  pilU Vibrio cholerae strain A1552

53.371

93.684

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.458

94.211

0.4
  pilT Acinetobacter baumannii strain A118

40.407

90.526

0.366
  pilT Acinetobacter nosocomialis M2

40.407

90.526

0.366
  pilT Acinetobacter baumannii D1279779

40.407

90.526

0.366
  pilT Pseudomonas aeruginosa PAK

40.896

88.158

0.361
  pilT Legionella pneumophila strain Lp02

40.896

88.158

0.361
  pilT Legionella pneumophila strain ERS1305867

40.896

88.158

0.361

Multiple sequence alignment