Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CKV94_RS10965 Genome accession   NZ_LT906482
Coordinates   2182750..2183790 (+) Length   346 a.a.
NCBI ID   WP_003822843.1    Uniprot ID   -
Organism   Eikenella corrodens strain NCTC10596     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2177750..2188790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV94_RS10940 (SAMEA4412678_02152) thiD 2178247..2179062 (-) 816 WP_003822835.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  CKV94_RS10945 (SAMEA4412678_02153) thiM 2179085..2179885 (-) 801 WP_003822837.1 hydroxyethylthiazole kinase -
  CKV94_RS10955 (SAMEA4412678_02154) greB 2180547..2181038 (-) 492 WP_003822840.1 transcription elongation factor GreB -
  CKV94_RS10960 (SAMEA4412678_02155) - 2181078..2182469 (-) 1392 WP_003822841.1 DHA2 family efflux MFS transporter permease subunit -
  CKV94_RS10965 (SAMEA4412678_02156) pilT 2182750..2183790 (+) 1041 WP_003822843.1 type IV pilus twitching motility protein PilT Machinery gene
  CKV94_RS10970 (SAMEA4412678_02157) pilU 2184319..2185509 (+) 1191 WP_223417339.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CKV94_RS10975 (SAMEA4412678_02158) rarD 2185778..2186677 (+) 900 WP_003822845.1 EamA family transporter RarD -
  CKV94_RS10980 (SAMEA4412678_02159) - 2186788..2188281 (-) 1494 WP_003822846.1 patatin-like phospholipase family protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38133.70 Da        Isoelectric Point: 7.4407

>NTDB_id=1032068 CKV94_RS10965 WP_003822843.1 2182750..2183790(+) (pilT) [Eikenella corrodens strain NCTC10596]
MQITDLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPEMSAEEVGNMIASIMNDHQRKDYQQRLETDFSFELPNIAR
FRVNAFNTERGPAAVFRTIPSKVLTLEELNAPKVFQKISDQPRGLVLVTGPTGSGKSTTLAAMINYINENHHHHILTIED
PIEFVHESKQCLVNQRELHQHTHSFANALRSALREDPDIILVGEMRDPETIGLALTAAETGHLVFATLHTTGAAKTVDRI
IDVFPAGEKEMVRSMLSESLRAVISQTLLKTKDGKGRVAAHEILISTAAVRNLIRENKIAQIGSALQTGQAHGMQTLDQS
LQALLKRGVISIDAARAKAQNPDQLV

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1032068 CKV94_RS10965 WP_003822843.1 2182750..2183790(+) (pilT) [Eikenella corrodens strain NCTC10596]
ATGCAGATTACCGACCTACTCGCGTTCAGCGTGAAAAACAAAGCATCCGACTTGCACCTCTCTGCCGGCCTGCCGCCGAT
GATCCGCGTGCACGGCGACGTGCGCCGCATCAACCTGCCGGAAATGAGCGCCGAAGAAGTGGGCAACATGATCGCTTCCA
TCATGAATGACCACCAGCGTAAAGACTACCAACAACGGCTGGAAACCGACTTTTCCTTCGAACTGCCCAACATCGCCCGT
TTCCGTGTGAACGCATTCAACACCGAACGCGGCCCGGCTGCCGTGTTCCGTACCATTCCCAGCAAAGTGCTCACGCTGGA
AGAGTTGAACGCGCCCAAAGTGTTCCAAAAAATTTCCGACCAACCGCGCGGCTTGGTGTTGGTAACCGGCCCTACCGGTT
CTGGTAAATCCACCACCCTGGCGGCGATGATCAACTACATCAACGAAAACCATCATCACCACATCCTCACCATCGAAGAC
CCGATCGAGTTTGTGCACGAAAGTAAGCAGTGTCTGGTAAACCAACGCGAACTGCACCAACACACCCACAGCTTCGCCAA
CGCCCTGCGCTCCGCATTGCGTGAAGACCCGGATATCATCCTGGTGGGTGAGATGCGTGACCCGGAAACCATCGGCTTGG
CGCTCACCGCTGCCGAAACCGGCCACTTGGTATTCGCCACCTTGCATACCACCGGTGCCGCCAAAACCGTGGACCGTATT
ATCGACGTGTTCCCCGCCGGCGAAAAAGAGATGGTGCGCTCCATGCTTTCCGAATCACTGCGCGCCGTGATTTCGCAAAC
CCTGCTCAAAACCAAAGACGGTAAAGGCCGTGTGGCCGCGCACGAAATCCTGATTTCCACCGCCGCCGTGCGTAACCTGA
TCCGCGAAAACAAAATCGCCCAAATCGGTTCTGCCCTGCAAACCGGCCAGGCGCATGGTATGCAGACGCTCGACCAATCC
TTGCAGGCACTGCTCAAACGTGGCGTTATCAGCATCGATGCTGCCCGTGCCAAAGCGCAGAACCCCGACCAACTCGTCTA
A

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

81.739

99.711

0.815
  pilT Neisseria meningitidis 8013

81.449

99.711

0.812
  pilT Acinetobacter baylyi ADP1

72.464

99.711

0.723
  pilT Pseudomonas stutzeri DSM 10701

72.464

99.711

0.723
  pilT Acinetobacter baumannii strain A118

72.254

100

0.723
  pilT Acinetobacter nosocomialis M2

72.254

100

0.723
  pilT Acinetobacter baumannii D1279779

72.254

100

0.723
  pilT Pseudomonas aeruginosa PAK

71.884

99.711

0.717
  pilT Legionella pneumophila strain Lp02

67.449

98.555

0.665
  pilT Legionella pneumophila strain ERS1305867

67.449

98.555

0.665
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.294

98.266

0.642
  pilT Vibrio cholerae strain A1552

65.294

98.266

0.642
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

99.711

0.497
  pilU Pseudomonas stutzeri DSM 10701

41.449

99.711

0.413
  pilU Vibrio cholerae strain A1552

40.87

99.711

0.408
  pilU Acinetobacter baylyi ADP1

39.595

100

0.396

Multiple sequence alignment