Detailed information NTG1032069

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CKV94_RS10970
• Genome accession: NZ_LT906482
• Coordinates: 2184319..2185509 (+)
• Length: 396 a.a.
• NCBI ID: WP_223417339.1
• Uniprot ID: -
• Organism: Eikenella corrodens strain NCTC10596
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2179319..2190509

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CKV94_RS10955 (SAMEA4412678_02154)	greB	2180547..2181038 (-)	492	WP_003822840.1	transcription elongation factor GreB	-
CKV94_RS10960 (SAMEA4412678_02155)	-	2181078..2182469 (-)	1392	WP_003822841.1	DHA2 family efflux MFS transporter permease subunit	-
CKV94_RS10965 (SAMEA4412678_02156)	pilT	2182750..2183790 (+)	1041	WP_003822843.1	type IV pilus twitching motility protein PilT	Machinery gene
CKV94_RS10970 (SAMEA4412678_02157)	pilU	2184319..2185509 (+)	1191	WP_223417339.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CKV94_RS10975 (SAMEA4412678_02158)	rarD	2185778..2186677 (+)	900	WP_003822845.1	EamA family transporter RarD	-
CKV94_RS10980 (SAMEA4412678_02159)	-	2186788..2188281 (-)	1494	WP_003822846.1	patatin-like phospholipase family protein	-
CKV94_RS10985 (SAMEA4412678_02160)	ppx	2188471..2189964 (+)	1494	WP_003822849.1	exopolyphosphatase	-

Sequence

Protein

• Length: 396 a.a.
• Molecular weight: 44939.31 Da
• Isoelectric Point: 6.4527

>NTDB_id=1032069 CKV94_RS10970 WP_223417339.1 2184319..2185509(+) (pilU) [Eikenella corrodens strain NCTC10596]
MHAAPAQPVGHGPNQHLTRERAKLHPLLEKMADESLKRNASDIFISSNFPPSFKIDGTLVPIPVKPLTEDEAAQIVYSTF
NDEQKAKFPVEWELNYSLQSQNGIRFRVNAYHEQGRIGLVMRRITTNILTIDDLRLPQVLKELSMRKRGLIILAGPTGSG
KSTSQAAMLDWRNKHSAGHIVTIEDPIEYIHSPIKSIITQREVGLDTHSWGAAVQSAMRQAPDVVCVGEVRNEHSMEYAL
QLAQTGHLCFFTIHATNASQTVERIMNLYPEERHPQILMDLALNLVAIIGQRLVLKKGGKGRNAIIDLLINTPAMQDHVF
KNQLLEARELMERAEDDGMQTFDQDLFRLYINNEIDYDEALRQAESANDLRLRIKLYEEGRESTHIFERVNDLNLM

Nucleotide

• Length: 1191 bp

>NTDB_id=1032069 CKV94_RS10970 WP_223417339.1 2184319..2185509(+) (pilU) [Eikenella corrodens strain NCTC10596]
CTGCATGCCGCGCCAGCACAGCCTGTCGGTCATGGGCCAAACCAGCACTTAACCCGTGAGCGTGCCAAACTGCACCCGCT
ACTGGAAAAAATGGCTGATGAATCGCTCAAACGCAATGCTTCTGATATCTTCATCAGCAGCAACTTCCCGCCTTCATTCA
AAATCGACGGCACGCTGGTACCGATTCCGGTAAAACCGCTCACTGAAGACGAAGCCGCACAAATCGTTTACTCCACCTTC
AACGACGAACAAAAAGCCAAATTCCCGGTAGAGTGGGAGCTGAACTACTCCTTGCAATCGCAAAACGGTATCCGTTTCCG
TGTGAATGCCTACCATGAACAAGGCCGCATCGGCTTGGTAATGCGCCGCATCACCACCAACATCCTCACCATCGACGACC
TCCGCCTGCCGCAAGTGCTCAAAGAGCTTTCCATGCGCAAGCGCGGCCTGATTATTCTGGCCGGCCCCACCGGTTCCGGT
AAATCCACTTCGCAGGCCGCGATGCTCGACTGGCGTAACAAACACTCTGCCGGCCACATCGTAACCATCGAAGACCCGAT
CGAGTATATCCACAGCCCGATCAAGAGCATCATCACCCAACGCGAAGTCGGCCTCGACACCCACAGCTGGGGCGCGGCCG
TACAAAGCGCCATGCGTCAGGCACCCGACGTAGTCTGCGTGGGTGAGGTGCGTAACGAACATTCCATGGAATATGCCCTG
CAATTGGCGCAAACCGGTCACTTATGTTTCTTCACCATCCACGCCACCAACGCCAGCCAAACCGTTGAGCGTATTATGAA
CTTGTATCCGGAAGAGCGGCATCCGCAAATTCTGATGGACTTGGCATTGAACTTGGTGGCCATTATCGGCCAGCGTTTGG
TGCTGAAAAAAGGCGGCAAAGGCCGAAATGCCATCATCGACTTGCTGATCAACACTCCCGCGATGCAGGACCACGTGTTC
AAAAACCAGCTGCTTGAGGCTCGTGAACTGATGGAACGCGCCGAGGACGACGGCATGCAAACCTTCGACCAAGACTTGTT
CCGCCTCTACATCAACAACGAAATCGACTACGACGAAGCCCTGCGCCAGGCCGAATCTGCCAACGACTTGCGCCTGCGCA
TCAAGCTCTACGAAGAAGGCCGCGAAAGCACGCATATCTTCGAACGCGTAAACGATTTGAACCTGATGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (30-294)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	47.765	90.404	0.432
pilU	Pseudomonas stutzeri DSM 10701	47.727	88.889	0.424
pilU	Vibrio cholerae strain A1552	43.391	87.879	0.381